Team:Peking

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Modeling
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Protocol
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Luminesensor Optimization
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Luminesensor
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Appendix
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Ring Pattern
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Sowing Tomorrow Synthetic Biologists
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Sowing Tomorrow's Synthetic Biologists
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   <div class="floatL"><p class="title_block" style="width:250px;height:250px;background-image:url('/wiki/images/b/be/Peking2012_Color_Title_AquaBlue.jpg');"><br /><br />Abstract</p></div>
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   <div style="float:left;"><p style="font-size:45px;line-height:45px;color:#ffffff;margin:20px 3px 0 20px;">L</p></div>
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Optogenetic tools have made significant impact on life sciences and beyond. However, several serious issues remain: cytotoxicy, narrow dynamic range, and dependency on laser and exogenous chromophore. To circumvent these, Peking iGEM has rationally constructed a hypersensitive sensor of luminance -- <i>Luminesensor</i>. Primarily, the sensor was designed by fusing blue-light-sensing protein domain from <i>Neurospora</i> with DNA binding domain of LexA from <i>E. coli</i>, following which protein structure inspection and kinetic simulation were conducted to rationally perform optimization. Amazingly, <i>Luminesensor</i> was proved to be as sensitive as to sense natural light and even bioluminescence. With this sensor, spatiotemporal control of cellular behavior, such as phototaxis, high-resolution 2D and 3D bio-printing using dim light and even luminescence of iPad were shown to be very easy. What’s more, we successfully implemented cell-cell signaling using light, which is the very first time in synthetic biology and of great importance for biotechnological use.
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ight has created innumerable wonders in nature. Equipped with tools and methods in optogenetic research, scientists have also achieved some truly fascinating goals in a synthetic way. This year, 2012 Peking-ECUST iGEM utilized an ultra-sensitive engineered sensor of luminescence -- the <b style="color:#ffee55;"> <i>Luminesensor</i></b>. Amazingly, the <i>Luminesensor</i> was proven to be so sensitive as to be able to detect <b style="color:#ffee55;">natural light</b> and even <b style="color:#ffee55;">bioluminescence</b>. With this sensor, spatiotemporal control of cellular behavior, such as <b style="color:#ffee55;">high-resolution 2D</b> and <b style="color:#ffee55;">3D bio-printing</b> using dim light and even the <b style="color:#ffee55;">luminescence of iPad</b> were shown to be very easy. What’s more, we successfully implemented <b style="color:#ffee55;">cell-cell signaling using light</b> for the very first time in synthetic biology, which will be of great importance for biotechnological use.
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<div class="floatL" style="margin:0 10px 5px 0;"><table class="title_block" style="background-image:url('/wiki/images/b/be/Peking2012_Color_Title_AquaBlue.jpg');"><tr><td style="height:244px;"><p style="font-size:36px;">Abstract</p></td></tr></table></div>
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   <div class="floatL"><p class="title_block" style="width:200px;height:150px;background-image:url('/wiki/images/a/a4/Peking2012_Color_Title_FlameYellow.jpg');"><br /><br />Luminesensor</p></div>
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   <div class="floatL"><table class="title_block" style="background-image:url('/wiki/images/a/a4/Peking2012_Color_Title_FlameYellow.jpg');"><tr><td><a href="/Team:Peking/Project/Luminesensor" style="font-size:26px;">Luminesensor</a></tr></td></table></div>
   <div class="floatR"><img src="/wiki/images/4/4f/Peking2012_LuminesensorMechanism.jpg" alt="" style="width:200px;"/></div>
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With strong motivation to raise a new generation of optogenetics, the Peking iGEM team has rationally constructed a hypersensitive sensor of luminance – what we call the <i>Luminesensor</i>. Primarily, the sensor was designed by fusing a blue-light-sensing protein domain from <i>Neurospora</i> with a DNA binding domain LexA from <i>E. coli</i>. Following this, protein structure inspection and kinetic simulations were conducted to rationally perform optimization. Amazingly, the <i>Luminesensor</i> proved to be as sensitive to sense natural light and even bioluminescence. With this sensor, spatiotemporal control of cellular behavior, such as phototaxis, high-resolution 2D and 3D bio-printing using dim light, and even utilizing the luminescence of an iPad were shown to be quite possible. What’s more, we successfully implemented cell-cell communication using light for the very first time in synthetic biology, which will prove to be of great importance for biotechnological use.  
+
Strongly motivated to open up a new era of optogenetics, our team has utilized an ultra-sensitive sensor which is engineered for sensing luminance – named by us <i>Luminesensor</i>, enabling highly efficient spatiotemporal control of biochemical process or cellular behavior. The whole job serves as a paradigm for sensor creating.
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   <div class="floatL"><p class="title_block" style="width:200px;height:150px;background-image:url('/wiki/images/a/a2/Peking2012_Color_Title_FreshGreen.jpg');"><br /><br />Light Communication</p></div>
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   <div class="floatL"><table class="title_block" style="background-image:url('/wiki/images/a/a2/Peking2012_Color_Title_FreshGreen.jpg');"><tr><td><a href="/Team:Peking/Project/Communication" style="font-size:26px;">Light <br /> Communication</a></tr></td></table></div>
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The ultrasensitive <i>Luminesensor</i> is able to respond to very dim light and still maintain a high dynamic range. That encouraged Peking iGEM to explore the possibility of cell-cell communication through light. Similar to other universal signals, the delivery of light signals is not limited by diffusion or by variation of organisms across species or even kingdoms. By carefully selecting the lux operon as the light sender module, Peking iGEM successfully demonstrated that Luminesensor is able to sense the blue light of the lux operon. This is the first time that light-communication between cells has been achieved without direct physical contact. As a proof of concept, a video was recorded to reveal the timing-course change of the sender and the receiver cells. Quantitative data was also obtained to evaluate the efficiency of light-communication. To build a complete light-communication system, a Light-On system was also constructed to achieve both positive and negative control by light. As the application of synthetic biology is becoming of age, Peking iGEM has probed into the bright future of light-communication.
+
The <i>Luminesensor</i> is able to respond to very low light and meanwhile keep a wide dynamic range, which encouraged us to explore the possibility of cell-cell communication through light. We have successfully implemented, for the very first time, light-communication among cells without direct physical contact.  
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   <div class="floatL"><p class="title_block" style="width:200px;height:200px;background-image:url('/wiki/images/f/fe/Peking2012_Color_Title_GrapePurple.jpg');"><br /><br />Syn Bio in 2D & 3D</p></div>
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   <div class="floatL"><table class="title_block" style="background-image:url('/wiki/images/f/fe/Peking2012_Color_Title_GrapePurple.jpg');"><tr><td><a href="/Team:Peking/Project/3D">Syn Bio in <br /> 2D & 3D</a></tr></td></table></div>
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3D printing is a new technology that has been rising for many years. The methods for 3D printing in manufacturing are quite developed, but in the realm of synthetic biology, there have been only a few attempts made. We believe that 3D printing can be utilized in many applications in both medical and manufacturing, such as with the synthesis of artificial vessels, organs, or bone tissues, and the creation of high-order biomaterials based on the high spatial resolution of the light. With more precise control of the response threshold of the optic biosensor, we can obtain holographic images from a solid medium.
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3D printing is a new technology that has been rising for many years. But in the realm of synthetic biology, it is far from developed. We exploited our <i>Luminesensor</i> to implement 3D printing that can be utilized in many medical or manufacturing applications.
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   <div class="floatL"><p class="title_block" style="width:200px;height:200px;background-image:url('/wiki/images/5/50/Peking2012_Color_Title_CherryPink.jpg');"><br /><br />Phototaxis</p></div>
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   <div class="floatL"><table class="title_block" style="background-image:url('/wiki/images/5/50/Peking2012_Color_Title_CherryPink.jpg');"><tr><td><a href="/Team:Peking/Project/Phototaxis">Phototaxis</a></tr></td></table></div>
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The 2012 Peking iGEM has successfully built "Phototatic" bacteria by programming the chemotaxis system in <i>E. coli</i> through light with the <i>Luminesensor</i>. By controlling the expression level of the cheZ protein with light, the tumbling frequency is coupled to the intensity of light signals. On the border of the light and dark fields on the plate, the motility difference of the cells in a single colony on the two sides is sufficient to form an uneven colony. The light-controlled cell motion that we have achieved has a very promising environmental and medical application for the future, <i>e.g.</i> the delivery of drugs to target concerns.  
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"Phototatic" bacteria can be built by programming the chemotaxis system in <i>E. coli</i> through light. By controlling the expression level of the CheZ protein with the <i>Luminesensor</i>, the tumbling frequency is coupled to the intensity of light signals.  
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We have done a remarkable job in motivating high school students to study synthetic biology and guiding them towards future participation in the iGEM high school division. Besides, we collaborated with a lab and helped four other iGEM teams by sharing DNA materials, characterizing their parts and modeling. Furthermore, we presented all fresh iGEMers with a collection of praise for historic iGEM projects.
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Excellent stochastic simulation was conducted to describe our ultrasensitive <i>Luminesensor</i>, combining protein kinetics and reaction thermodynamics. Molecular docking was also operated. What's more, while designing our photo-taxis model, we developed a hexagonal-coordinate environment for dynamic simulation of "on-plate" system. Those who focus on cell motility will find it useful and inspirational.   
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Latest revision as of 20:16, 26 October 2012

L

ight has created innumerable wonders in nature. Equipped with tools and methods in optogenetic research, scientists have also achieved some truly fascinating goals in a synthetic way. This year, 2012 Peking-ECUST iGEM utilized an ultra-sensitive engineered sensor of luminescence -- the Luminesensor. Amazingly, the Luminesensor was proven to be so sensitive as to be able to detect natural light and even bioluminescence. With this sensor, spatiotemporal control of cellular behavior, such as high-resolution 2D and 3D bio-printing using dim light and even the luminescence of iPad were shown to be very easy. What’s more, we successfully implemented cell-cell signaling using light for the very first time in synthetic biology, which will be of great importance for biotechnological use.

Abstract

Luminesensor

Strongly motivated to open up a new era of optogenetics, our team has utilized an ultra-sensitive sensor which is engineered for sensing luminance – named by us Luminesensor, enabling highly efficient spatiotemporal control of biochemical process or cellular behavior. The whole job serves as a paradigm for sensor creating.

Light
Communication

The Luminesensor is able to respond to very low light and meanwhile keep a wide dynamic range, which encouraged us to explore the possibility of cell-cell communication through light. We have successfully implemented, for the very first time, light-communication among cells without direct physical contact.

Syn Bio in
2D & 3D

3D printing is a new technology that has been rising for many years. But in the realm of synthetic biology, it is far from developed. We exploited our Luminesensor to implement 3D printing that can be utilized in many medical or manufacturing applications.

Phototaxis

"Phototatic" bacteria can be built by programming the chemotaxis system in E. coli through light. By controlling the expression level of the CheZ protein with the Luminesensor, the tumbling frequency is coupled to the intensity of light signals.

Human
Practice

We have done a remarkable job in motivating high school students to study synthetic biology and guiding them towards future participation in the iGEM high school division. Besides, we collaborated with a lab and helped four other iGEM teams by sharing DNA materials, characterizing their parts and modeling. Furthermore, we presented all fresh iGEMers with a collection of praise for historic iGEM projects.

Modeling

Excellent stochastic simulation was conducted to describe our ultrasensitive Luminesensor, combining protein kinetics and reaction thermodynamics. Molecular docking was also operated. What's more, while designing our photo-taxis model, we developed a hexagonal-coordinate environment for dynamic simulation of "on-plate" system. Those who focus on cell motility will find it useful and inspirational.

Retrieved from "http://2012.igem.org/Team:Peking"