http://2012.igem.org/wiki/index.php?title=Team:NTU-Taida/Safety&feed=atom&action=historyTeam:NTU-Taida/Safety - Revision history2024-03-29T11:20:27ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:NTU-Taida/Safety&diff=296165&oldid=prevShihyi: /* Question 4 */2012-10-27T02:29:20Z<p><span class="autocomment">Question 4</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As our opinion, we think that for every iGEM teams' circuit design, a mechanism to turn off is necessary. In order to improve the iGEM competition safety concern, we would like to suggest a turn-off mechanism as requirement. Also, the microorganisms should be kept dependent on controlled conditions in the lab to prohibit the unwanted spreading and multiplication outside the lab. Moreover, a suicide switch for all BioBricks or dependence on special nutrients lack in nature for organisms should be engineered in case organisms escape and cause safety concern. The most important thing is that every team and the advisor should know their potential danger and the ways to assess the risk. This aim could be approached by emphasizing the safety rules. Creating a page on the registry for the teams to explain their event tree analysis and fault tree analysis would be an easiest method to increase safety when working with BioBrick parts.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As our opinion, we think that for every iGEM teams' circuit design, a mechanism to turn off is necessary. In order to improve the iGEM competition safety concern, we would like to suggest a turn-off mechanism as requirement. Also, the microorganisms should be kept dependent on controlled conditions in the lab to prohibit the unwanted spreading and multiplication outside the lab. Moreover, a suicide switch for all BioBricks or dependence on special nutrients lack in nature for organisms should be engineered in case organisms escape and cause safety concern. The most important thing is that every team and the advisor should know their potential danger and the ways to assess the risk. This aim could be approached by emphasizing the safety rules. Creating a page on the registry for the teams to explain their event tree analysis and fault tree analysis would be an easiest method to increase safety when working with BioBrick parts.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>For our PepdEx system, we proposed two layers of safety regulations: (1) <del class="diffchange diffchange-inline">temperature inducible </del>suicide <del class="diffchange diffchange-inline">system </del>to prevent gene recombination/pollution, <del class="diffchange diffchange-inline">and </del>(2) RNA interaction/competitive inhibition to prevent horizontal gene transfer. Detailed descriptions can be found in our "Project" section.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>For our PepdEx system, we proposed two layers of safety regulations: (1) suicide <ins class="diffchange diffchange-inline">device </ins>to prevent gene recombination/pollution, (2) RNA interaction/competitive inhibition to prevent horizontal gene transfer<ins class="diffchange diffchange-inline">, and (3) traditinal antibiotics use and fecal microbiota transplantation</ins>. Detailed descriptions can be found in our "Project" section.</div></td></tr>
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</table>Shihyihttp://2012.igem.org/wiki/index.php?title=Team:NTU-Taida/Safety&diff=296125&oldid=prevShihyi at 02:28, 27 October 20122012-10-27T02:28:00Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For our PepdEx system, we proposed two layers of safety regulations: (1) temperature inducible suicide system to prevent gene recombination/pollution, and (2) RNA interaction/competitive inhibition to prevent horizontal gene transfer. Detailed descriptions can be found in our "Project" section.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For our PepdEx system, we proposed two layers of safety regulations: (1) temperature inducible suicide system to prevent gene recombination/pollution, and (2) RNA interaction/competitive inhibition to prevent horizontal gene transfer. Detailed descriptions can be found in our "Project" section.</div></td></tr>
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</table>Shihyihttp://2012.igem.org/wiki/index.php?title=Team:NTU-Taida/Safety&diff=296113&oldid=prevShihyi: /* Question 4 */2012-10-27T02:27:38Z<p><span class="autocomment">Question 4</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For our PepdEx system, we proposed two layers of safety regulations: (1) temperature inducible suicide system to prevent gene recombination/pollution, and (2) RNA interaction/competitive inhibition to prevent horizontal gene transfer. Detailed descriptions can be found in our "Project" section.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For our PepdEx system, we proposed two layers of safety regulations: (1) temperature inducible suicide system to prevent gene recombination/pollution, and (2) RNA interaction/competitive inhibition to prevent horizontal gene transfer. Detailed descriptions can be found in our "Project" section.</div></td></tr>
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</table>Shihyihttp://2012.igem.org/wiki/index.php?title=Team:NTU-Taida/Safety&diff=296019&oldid=prevShihyi: /* Testing Function */2012-10-27T02:24:11Z<p><span class="autocomment">Testing Function</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For our PepdEx system, we proposed two layers of safety regulations: (1) temperature inducible suicide system to prevent gene recombination/pollution, and (2) RNA interaction/competitive inhibition to prevent horizontal gene transfer. Detailed descriptions can be found in our "Project" section.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For our PepdEx system, we proposed two layers of safety regulations: (1) temperature inducible suicide system to prevent gene recombination/pollution, and (2) RNA interaction/competitive inhibition to prevent horizontal gene transfer. Detailed descriptions can be found in our "Project" section.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">== Testing Function ==</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">$$x = {-b \pm \sqrt{b^2-4ac} \over 2a}.$$</del></div></td><td colspan="2"> </td></tr>
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</table>Shihyihttp://2012.igem.org/wiki/index.php?title=Team:NTU-Taida/Safety&diff=291777&oldid=prevLbwang: /* Testing Function */2012-10-26T23:16:51Z<p><span class="autocomment">Testing Function</span></p>
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</table>Lbwanghttp://2012.igem.org/wiki/index.php?title=Team:NTU-Taida/Safety&diff=291775&oldid=prevLbwang: /* Testing Function */2012-10-26T23:16:39Z<p><span class="autocomment">Testing Function</span></p>
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</table>Lbwanghttp://2012.igem.org/wiki/index.php?title=Team:NTU-Taida/Safety&diff=291356&oldid=prevLbwang: /* Testing Function */2012-10-26T22:53:11Z<p><span class="autocomment">Testing Function</span></p>
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</table>Lbwanghttp://2012.igem.org/wiki/index.php?title=Team:NTU-Taida/Safety&diff=291351&oldid=prevLbwang: /* Testing Function */2012-10-26T22:52:57Z<p><span class="autocomment">Testing Function</span></p>
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</table>Lbwanghttp://2012.igem.org/wiki/index.php?title=Team:NTU-Taida/Safety&diff=288791&oldid=prevJasmine: /* Question 1 */2012-10-26T19:54:03Z<p><span class="autocomment">Question 1</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''environmental safety?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''environmental safety?'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The biosafety concerns can be simplified to an equation, Risk= probabilityX Hazard. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The biosafety concerns can be simplified to an equation, Risk= probabilityX Hazard. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>In our project, the questions we deal with <del class="diffchange diffchange-inline">is </del>basically human disease-oriented. We try to prevent any concern regarding potential harms to human. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>In our project, the questions we deal with <ins class="diffchange diffchange-inline">are </ins>basically human disease-oriented. We try to prevent any concern regarding potential harms to human. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As for the researchers, our research is conducted in a safely regulated laboratory, and the ideas of our project do not require etiologic agents, hosts or vectors for experiment. Also no toxic reagent or high risk chemical compound is used. In all, our project hasn’t yet raised researcher safety issues thus far. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As for the researchers, our research is conducted in a safely regulated laboratory, and the ideas of our project do not require etiologic agents, hosts or vectors for experiment. Also no toxic reagent or high risk chemical compound is used. In all, our project hasn’t yet raised researcher safety issues thus far. </div></td></tr>
</table>Jasminehttp://2012.igem.org/wiki/index.php?title=Team:NTU-Taida/Safety&diff=287982&oldid=prevJasmine: /* Question 3 */2012-10-26T18:59:17Z<p><span class="autocomment">Question 3</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In National Taiwan University, College of Medicine, where the experiments of our project are conducted, Environmental Protection and Occupational Safety and Hygiene Unit of NTUCM takes charge of promoting and supervising safety and health issues.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In National Taiwan University, College of Medicine, where the experiments of our project are conducted, Environmental Protection and Occupational Safety and Hygiene Unit of NTUCM takes charge of promoting and supervising safety and health issues.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Before beginning of our project, our team members and advisors have attended the safety education series and training courses held by this unit. All of us were qualified by passing the tests at the end of the training to be sure that all the rules of concern are well understood. The application of our project was also verified and qualified. All experiments are conducted under the biosafety rules of Environmental Protection and Occupational Safety and Hygiene Unit of NTUCM, of which all of our experimental procedures, <del class="diffchange diffchange-inline">equipements</del>, facilities were under close supervision.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Before beginning of our project, our team members and advisors have attended the safety education series and training courses held by this unit. All of us were qualified by passing the tests at the end of the training to be sure that all the rules of concern are well understood. The application of our project was also verified and qualified. All experiments are conducted under the biosafety rules of Environmental Protection and Occupational Safety and Hygiene Unit of NTUCM, of which all of our experimental procedures, <ins class="diffchange diffchange-inline">equipments</ins>, facilities were under close supervision.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.mc.ntu.edu.tw/department/safety/index.htm</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>http://www.mc.ntu.edu.tw/department/safety/index.htm</div></td></tr>
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</table>Jasmine