Team:NTU-Taida/Modeling/Parameters

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Single-Cell/ System analysis Model

Parameter

Description 

Value 

Reference

cd

E. coli Cell Density

0.1

 

DFA

FA Diffusion Constant

6.46* 10^-10m^2 /s

DAHL

AHL Diffusion Constant

4.9e-6 cm2/s

[9]

γAHL,ext

AHL cell-external degradation

8.0225e-006/s

Derived from 1 day half-life at pH 7 <a href="http://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref7" title="Team:ETH Zurich/Modeling/References">[7]</a>

Fatty Acid Reaction Absorption Model Model

Parameter

Description 

Value 

Reference

[E]t

Total active enzyme

50 uM

[8]

Km

Reaction rate constant

47.9

[6][7]

DFA

FA Diffusion Constant

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d

Thickness of the unstirred water layer

190 um

[9]

Kcat/ molecular weight

Catalytic Rate constant

1.8*10^-3

[6][7]

Cell-Cell Communication Model

Parameter

Description 

Value 

Reference

cd

E. coli Cell Density

0.1

 

DFA

FA Diffusion Constant

6.46* 10^-10m^2 /s

DAHL

AHL Diffusion Constant

4.9e-6 cm2/s

[9]

γAHL,ext

AHL cell-external degradation

8.0225e-006/s

Derived from 1 day half-life at pH 7 <a href="http://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref7" title="Team:ETH Zurich/Modeling/References">[7]</a>



Reference

Source

[1]

Subhayu Basu, Yoram Gerchman, Cynthia H. Collins, Frances H. Arnold & Ron Weiss.A synthetic multicellular system for programmed pattern formation, Nature Vol. 434, 2005

[2]

Garcia-Ojalvo, Michael B. Elowitz, and Steven H. Strogatz. Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing, PNAS vol. 101 no. 30, 2004

[3]

MIT igem 2010

[4]

Michael Halter, Alex Tona, Kiran Bhadriraju, Anne L. Plant, John T. Elliott, Automated Live Cell Imaging of Green Fluorescent Protein Degradation in Individual Fibroblasts, Cytometry Part A, Volume 71A Issue 10, 2007

[5]

Wilfried Weber, Markus Rimann, Manuela Spielmann, Bettina Keller, Marie Daoud-El Baba, Dominique Aubel, Cornelia C Weber & Martin Fussenegger, Gas-inducible transgene expression in mammalian cells and mice, Nature Biotechnology, volume 22, number 11, 2004

[6]

Sulaiman Al-Zuhair, Masitah Hasan, K.B. Ramachandran, Kinetics of the enzymatic hydrolysis of palm oil by lipase, Process Biochemistry

Volume 38, Issue 8, 2003

[7]

Ho-Shing Wu, Ming-Ju Tsai, Kinetics of tributyrin hydrolysis by lipase, Enzyme and Microbial Technology, Volume 35, Issues 6–7, 2004

[8]

Bengt Borgstrom, Luminal Digestion of Fats, Handbook of Physiology, The Gastrointestinal System, Intestinal Absorption and Secretion, 1991

[9]

Sallee VL, Dietschy JM., Determinants of intestinal mucosal uptake of short- and medium-chain fatty acids and alcohols, Journal of Lipid Research, 1973