Team:NTU-Taida/Modeling/Parameters

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==Single-Cell/ System analysis Model==
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{{:Team:NTU-Taida/Templates/BSHero|Title=Modeling Parameters|Content=<p>TEXT HERE</p>
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{|  class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="502" style="margin-left:.65pt;border-collapse:collapse;mso-table-layout-alt:fixed; mso-yfti-tbllook:1184;mso-padding-alt:0cm 1.4pt 0cm 1.4pt"  style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:17.25pt"
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}}
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|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;background:#F2F2F2;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Parameter</span></b></p>
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|  width="260" style="width:194.85pt;border:solid #AAAAAA 1.0pt;border-left:  none;background:#F2F2F2;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Description&nbsp;</span></b></p>
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|  width="104" style="width:77.95pt;border:solid #AAAAAA 1.0pt;border-left:  none;background:#F2F2F2;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Value&nbsp;</span></b></p>
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| width="66" style="width:49.6pt;border:solid #AAAAAA 1.0pt;border-left:none;  background:#F2F2F2;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Reference</span></b></p>
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|-  style="mso-yfti-irow:1;height:17.25pt"
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|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">cd</span></p>
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| width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><i><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; background-position: initial initial; background-repeat: initial initial; ">E. coli</span></i><span class="apple-converted-space"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; background-position: initial initial; background-repeat: initial initial; ">&nbsp;</span><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; background-position: initial initial; background-repeat: initial initial; ">Cell Density</span><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; "></span></span></p>
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|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">0.1</span></p>
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|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US">&nbsp;</span></p>
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|-  style="mso-yfti-irow:2;height:17.25pt"
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|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">D<sub>FA</sub></span></p>
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|  width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">FA </span><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; background-position: initial initial; background-repeat: initial initial; ">Diffusion Constant</span><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; "></span></p>
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|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">6.46* 10^-10m^2 /s</span></p>
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|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" |
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|-  style="mso-yfti-irow:3;height:17.25pt"
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|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">D<sub>AHL</sub></span></p>
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|  width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">AHL Diffusion Constant</span></p>
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|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">4.9e-6 cm2/s</span></p>
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|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">[9]</span></p>
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|-  style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:17.25pt"
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|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">γAHL,ext</span></p>
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|  width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">AHL cell-external degradation</span></p>
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|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">8.0225e-006/s</span></p>
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|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Derived from 1
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day half-life at pH 7&nbsp;<a href="http://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref7" title="Team:ETH Zurich/Modeling/References"><span style="color:black;
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text-decoration:none;text-underline:none">[7]</span></a></span></p>
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|}
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==Fatty Acid Reaction Absorption Model Model==
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{|  class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="502" style="margin-left:.65pt;border-collapse:collapse;mso-table-layout-alt:fixed; mso-yfti-tbllook:1184;mso-padding-alt:0cm 1.4pt 0cm 1.4pt"  style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:17.25pt"
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|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;background:#F2F2F2;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Parameter</span></b></p>
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|  width="260" style="width:194.85pt;border:solid #AAAAAA 1.0pt;border-left:  none;background:#F2F2F2;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Description&nbsp;</span></b></p>
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|  width="104" style="width:77.95pt;border:solid #AAAAAA 1.0pt;border-left:  none;background:#F2F2F2;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Value&nbsp;</span></b></p>
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|  width="66" style="width:49.6pt;border:solid #AAAAAA 1.0pt;border-left:none;  background:#F2F2F2;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Reference</span></b></p>
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|-  style="mso-yfti-irow:1;height:17.25pt"
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|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US">[E]<sub>t</sub></span><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; "></span></p>
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|  width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Total active enzyme</span></p>
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|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US">50 uM</span><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; "></span></p>
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|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US">[8]</span></p>
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|-  style="mso-yfti-irow:2;height:17.25pt"
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|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US">K<sub>m</sub></span><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; "></span></p>
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|  width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Reaction rate constant</span></p>
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|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">47.9</span></p>
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|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US">[6][7]</span></p>
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|-  style="mso-yfti-irow:3;height:17.25pt"
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|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">D<sub>FA</sub></span></p>
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|  width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">FA Diffusion Constant</span></p>
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|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">6.46* </span><&#33;--[if gte msEquation 12]><m:oMath><m:sSup><m:sSupPr><span
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font-family:"Cambria Math","serif";mso-ascii-font-family:"Cambria Math";
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mso-bidi-font-family:Arial;color:black;mso-font-kerning:0pt'><m:ctrlPr></m:ctrlPr></span></m:sSupPr><m:e><span
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lang=EN-US style='font-size:10.0pt;font-family:"Cambria Math","serif";
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mso-fareast-font-family:新細明體;mso-bidi-font-family:Arial;color:black;
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mso-font-kerning:0pt'><m:r><m:rPr><m:scr m:val="roman"/><m:sty m:val="p"/></m:rPr>10</m:r></span></m:e><m:sup><span
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lang=EN-US style='font-size:10.0pt;font-family:"Cambria Math","serif";
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mso-fareast-font-family:新細明體;mso-bidi-font-family:Arial;color:black;
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mso-font-kerning:0pt'><m:r><m:rPr><m:scr m:val="roman"/><m:sty m:val="p"/></m:rPr>-</m:r><m:r><m:rPr><m:scr
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m:val="roman"/><m:sty m:val="p"/></m:rPr>10</m:r></span></m:sup></m:sSup><m:sSup><m:sSupPr><span
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style='font-size:10.0pt;mso-ansi-font-size:10.0pt;mso-bidi-font-size:10.0pt;
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font-family:"Cambria Math","serif";mso-ascii-font-family:"Cambria Math";
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mso-fareast-font-family:新細明體;mso-hansi-font-family:"Cambria Math";
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mso-bidi-font-family:Arial;color:black;mso-font-kerning:0pt'><m:ctrlPr></m:ctrlPr></span></m:sSupPr><m:e><span
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lang=EN-US style='font-size:10.0pt;font-family:"Cambria Math","serif";
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mso-fareast-font-family:新細明體;mso-bidi-font-family:Arial;color:black;
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mso-font-kerning:0pt'><m:r><m:rPr><m:scr m:val="roman"/><m:sty m:val="p"/></m:rPr>m</m:r></span></m:e><m:sup><span
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lang=EN-US style='font-size:10.0pt;font-family:"Cambria Math","serif";
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mso-fareast-font-family:新細明體;mso-bidi-font-family:Arial;color:black;
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mso-font-kerning:0pt'><m:r><m:rPr><m:scr m:val="roman"/><m:sty m:val="p"/></m:rPr>2</m:r></span></m:sup></m:sSup><span
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lang=EN-US style='font-size:10.0pt;font-family:"Cambria Math","serif";
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mso-fareast-font-family:新細明體;mso-bidi-font-family:Arial;color:black;
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mso-font-kerning:0pt'><m:r><m:rPr><m:scr m:val="roman"/><m:sty m:val="p"/></m:rPr>
 +
/s</m:r></span></m:oMath><&#33;[endif]--><&#33;--[if &#33;msEquation]--><span lang="EN-US" style="font-size:12.0pt;mso-bidi-font-size:11.0pt;font-family:&quot;Calibri&quot;,&quot;sans-serif&quot;;
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mso-ascii-theme-font:minor-latin;mso-fareast-font-family:新細明體;mso-fareast-theme-font:
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minor-fareast;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:&quot;Times New Roman&quot;;
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mso-bidi-theme-font:minor-bidi;position:relative;top:2.5pt;mso-text-raise:
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-2.5pt;mso-ansi-language:EN-US;mso-fareast-language:ZH-TW;mso-bidi-language:
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AR-SA"><&#33;--[if gte vml 1]><v:shapetype id="_x0000_t75" coordsize="21600,21600"
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stroked="f"><v:stroke joinstyle="miter"/><v:formulas><v:f eqn="if lineDrawn pixelLineWidth 0"/><v:f eqn="sum @0 1 0"/><v:f eqn="sum 0 0 @1"/><v:f eqn="prod @2 1 2"/><v:f eqn="prod @3 21600 pixelWidth"/><v:f eqn="prod @3 21600 pixelHeight"/><v:f eqn="sum @0 0 1"/><v:f eqn="prod @6 1 2"/><v:f eqn="prod @7 21600 pixelWidth"/><v:f eqn="sum @8 21600 0"/><v:f eqn="prod @7 21600 pixelHeight"/><v:f eqn="sum @10 21600 0"/></v:formulas><v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/><o:lock v:ext="edit" aspectratio="t"/></v:shapetype><v:shape id="_x0000_i1025" type="#_x0000_t75" style='width:48.75pt;
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o:title="" chromakey="white"/></v:shape><&#33;[endif]--><&#33;--[if &#33;vml]--><img width="65" height="16" src="file:///C:/Users/curtis/AppData/Local/Temp/msohtmlclip1/01/clip_image002.png" v:shapes="_x0000_i1025"><&#33;--[endif]--></span><&#33;--[endif]--><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; "></span></p>
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|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">&nbsp;</span></p>
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|-  style="mso-yfti-irow:4;height:17.25pt"
 +
|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US">d</span><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; "></span></p>
 +
|  width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Thickness of the unstirred water layer</span></p>
 +
|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">190 um</span></p>
 +
|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US">[9]</span></p>
 +
|-  style="mso-yfti-irow:5;mso-yfti-lastrow:yes;height:17.25pt"
 +
|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Kcat/ molecular weight</span></p>
 +
|  width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Catalytic Rate constant</span></p>
 +
|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">1.8*10^-3</span></p>
 +
|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US">[6][7]</span></p>
 +
|}
-
{{:Team:NTU-Taida/Templates/ContentEnd}}{{:Team:NTU-Taida/Templates/Footer|ActiveNavbar=Modeling, #nav-Modeling-Parameters}}
+
==Cell-Cell Communication Model==
 +
{|  class="MsoNormalTable" border="0" cellspacing="0" cellpadding="0" width="502" style="margin-left:.65pt;border-collapse:collapse;mso-table-layout-alt:fixed; mso-yfti-tbllook:1184;mso-padding-alt:0cm 1.4pt 0cm 1.4pt"  style="mso-yfti-irow:0;mso-yfti-firstrow:yes;height:17.25pt"
 +
|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;background:#F2F2F2;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Parameter</span></b></p>
 +
|  width="260" style="width:194.85pt;border:solid #AAAAAA 1.0pt;border-left:  none;background:#F2F2F2;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Description&nbsp;</span></b></p>
 +
| width="104" style="width:77.95pt;border:solid #AAAAAA 1.0pt;border-left:  none;background:#F2F2F2;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Value&nbsp;</span></b></p>
 +
|  width="66" style="width:49.6pt;border:solid #AAAAAA 1.0pt;border-left:none;  background:#F2F2F2;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Reference</span></b></p>
 +
|-  style="mso-yfti-irow:1;height:17.25pt"
 +
|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">cd</span></p>
 +
|  width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><i><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; background-position: initial initial; background-repeat: initial initial; ">E. coli</span></i><span class="apple-converted-space"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; background-position: initial initial; background-repeat: initial initial; ">&nbsp;</span><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; background-position: initial initial; background-repeat: initial initial; ">Cell Density</span><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; "></span></span></p>
 +
|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">0.1</span></p>
 +
|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US">&nbsp;</span></p>
 +
|-  style="mso-yfti-irow:2;height:17.25pt"
 +
|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">D<sub>FA</sub></span></p>
 +
|  width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">FA </span><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; background-position: initial initial; background-repeat: initial initial; ">Diffusion Constant</span><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; "></span></p>
 +
|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">6.46* 10^-10m^2 /s</span></p>
 +
|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" |
 +
|-  style="mso-yfti-irow:3;height:17.25pt"
 +
|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">D<sub>AHL</sub></span></p>
 +
|  width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">AHL Diffusion Constant</span></p>
 +
|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">4.9e-6 cm2/s</span></p>
 +
|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">[9]</span></p>
 +
|-  style="mso-yfti-irow:4;mso-yfti-lastrow:yes;height:17.25pt"
 +
|  width="72" style="width:54.0pt;border:solid #AAAAAA 1.0pt;border-top:none;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">γAHL,ext</span></p>
 +
|  width="260" style="width:194.85pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">AHL cell-external degradation</span></p>
 +
|  width="104" style="width:77.95pt;border-top:none;border-left:none;  border-bottom:solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;  background:#F9F9F9;padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">8.0225e-006/s</span></p>
 +
|  width="66" style="width:49.6pt;border-top:none;border-left:none;border-bottom:  solid #AAAAAA 1.0pt;border-right:solid #AAAAAA 1.0pt;background:#F9F9F9;  padding:0cm 1.4pt 0cm 1.4pt;height:17.25pt" | <p class="MsoNormal" align="center" style="text-align:center;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Derived from 1
 +
day half-life at pH 7&nbsp;<a href="http://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref7" title="Team:ETH Zurich/Modeling/References"><span style="color:black;
 +
text-decoration:none;text-underline:none">[7]</span></a></span></p>
 +
|}
 +
 
 +
 
 +
----
 +
 
 +
==Reference==
 +
<html>
 +
<table class="MsoNormalTable" border="1" cellspacing="0" cellpadding="0" width="90%" style="width:90.0%;background:#F9F9F9;border-collapse:collapse;border:none;
 +
mso-border-alt:solid #AAAAAA .75pt;mso-yfti-tbllook:1184">
 +
<tbody><tr>
 +
  <td width="21" nowrap="" style="width:15.95pt;border:solid #AAAAAA 1.0pt;
 +
  mso-border-alt:solid #AAAAAA .75pt;background:#F2F2F2;padding:2.4pt 2.4pt 2.4pt 2.4pt"></td>
 +
  <td style="border:solid #AAAAAA 1.0pt;border-left:none;mso-border-left-alt:
 +
  solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;background:#F2F2F2;
 +
  padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:14.25pt;
 +
  mso-pagination:widow-orphan"><b><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">Source<o:p></o:p></span></b></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td style="border:solid #AAAAAA 1.0pt;border-top:none;mso-border-top-alt:
 +
  solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:14.25pt;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">[1]<o:p></o:p></span></p>
 +
  </td>
 +
  <td style="border-top:none;border-left:none;border-bottom:solid #AAAAAA 1.0pt;
 +
  border-right:solid #AAAAAA 1.0pt;mso-border-top-alt:solid #AAAAAA .75pt;
 +
  mso-border-left-alt:solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;
 +
  padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" style="line-height:14.25pt;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;;
 +
  mso-fareast-font-family:新細明體;mso-font-kerning:0pt">Subhayu Basu, Yoram
 +
  Gerchman, Cynthia H. Collins, Frances H. Arnold &amp; Ron Weiss.<b>A
 +
  synthetic multicellular system for programmed pattern formation</b>,&nbsp;Nature
 +
  Vol. 434, 2005<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td style="border:solid #AAAAAA 1.0pt;border-top:none;mso-border-top-alt:
 +
  solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:14.25pt;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">[2]<o:p></o:p></span></p>
 +
  </td>
 +
  <td style="border-top:none;border-left:none;border-bottom:solid #AAAAAA 1.0pt;
 +
  border-right:solid #AAAAAA 1.0pt;mso-border-top-alt:solid #AAAAAA .75pt;
 +
  mso-border-left-alt:solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;
 +
  padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" style="line-height:14.25pt;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;;
 +
  mso-fareast-font-family:新細明體;mso-font-kerning:0pt">Garcia-Ojalvo, Michael B. Elowitz,
 +
  and Steven H. Strogatz<b>. Modeling a synthetic multicellular clock:
 +
  Repressilators coupled by quorum sensing</b>,&nbsp;PNAS vol. 101 no. 30, 2004<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td style="border:solid #AAAAAA 1.0pt;border-top:none;mso-border-top-alt:
 +
  solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:14.25pt;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">[3]<o:p></o:p></span></p>
 +
  </td>
 +
  <td style="border-top:none;border-left:none;border-bottom:solid #AAAAAA 1.0pt;
 +
  border-right:solid #AAAAAA 1.0pt;mso-border-top-alt:solid #AAAAAA .75pt;
 +
  mso-border-left-alt:solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;
 +
  padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" style="line-height:14.25pt;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;;
 +
  mso-fareast-font-family:新細明體;mso-font-kerning:0pt">MIT igem 2010<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td style="border:solid #AAAAAA 1.0pt;border-top:none;mso-border-top-alt:
 +
  solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:14.25pt;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">[4]<o:p></o:p></span></p>
 +
  </td>
 +
  <td style="border-top:none;border-left:none;border-bottom:solid #AAAAAA 1.0pt;
 +
  border-right:solid #AAAAAA 1.0pt;mso-border-top-alt:solid #AAAAAA .75pt;
 +
  mso-border-left-alt:solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;
 +
  padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" style="line-height:14.25pt;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;;
 +
  mso-fareast-font-family:新細明體;mso-font-kerning:0pt">Michael Halter, Alex Tona,
 +
  Kiran Bhadriraju, Anne L. Plant, John T. Elliott,<b> Automated Live Cell
 +
  Imaging of Green Fluorescent Protein Degradation in Individual Fibroblasts</b>,
 +
  Cytometry Part A, Volume 71A Issue 10, 2007<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td style="border:solid #AAAAAA 1.0pt;border-top:none;mso-border-top-alt:
 +
  solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:14.25pt;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">[5]<o:p></o:p></span></p>
 +
  </td>
 +
  <td style="border-top:none;border-left:none;border-bottom:solid #AAAAAA 1.0pt;
 +
  border-right:solid #AAAAAA 1.0pt;mso-border-top-alt:solid #AAAAAA .75pt;
 +
  mso-border-left-alt:solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;
 +
  padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" style="line-height:14.25pt;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;;
 +
  mso-fareast-font-family:新細明體;mso-font-kerning:0pt">Wilfried Weber, Markus
 +
  Rimann, Manuela Spielmann, Bettina Keller, Marie Daoud-El Baba, Dominique
 +
  Aubel, Cornelia C Weber &amp; Martin Fussenegger,&nbsp;<b>Gas-inducible
 +
  transgene expression in mammalian cells and mice, Nature Biotechnology</b>, volume
 +
  22, number 11, 2004<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td style="border:solid #AAAAAA 1.0pt;border-top:none;mso-border-top-alt:
 +
  solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:14.25pt;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">[6]<o:p></o:p></span></p>
 +
  </td>
 +
  <td style="border-top:none;border-left:none;border-bottom:solid #AAAAAA 1.0pt;
 +
  border-right:solid #AAAAAA 1.0pt;mso-border-top-alt:solid #AAAAAA .75pt;
 +
  mso-border-left-alt:solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;
 +
  padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" style="line-height:14.25pt;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;;
 +
  mso-fareast-font-family:新細明體;mso-font-kerning:0pt">Sulaiman Al-Zuhair,
 +
  Masitah Hasan, K.B. Ramachandran, <b>Kinetics of the enzymatic hydrolysis of
 +
  palm oil by lipase</b>, Process Biochemistry<o:p></o:p></span></p>
 +
  <p class="MsoNormal" style="line-height:14.25pt;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;;
 +
  mso-fareast-font-family:新細明體;mso-font-kerning:0pt">Volume 38, Issue 8, 2003<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td style="border:solid #AAAAAA 1.0pt;border-top:none;mso-border-top-alt:
 +
  solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:14.25pt;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">[7]<o:p></o:p></span></p>
 +
  </td>
 +
  <td style="border-top:none;border-left:none;border-bottom:solid #AAAAAA 1.0pt;
 +
  border-right:solid #AAAAAA 1.0pt;mso-border-top-alt:solid #AAAAAA .75pt;
 +
  mso-border-left-alt:solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;
 +
  padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" style="line-height:14.25pt;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;;
 +
  mso-fareast-font-family:新細明體;mso-font-kerning:0pt">Ho-Shing Wu, Ming-Ju Tsai,
 +
  <b>Kinetics of tributyrin hydrolysis by
 +
  lipase</b>, Enzyme and Microbial Technology, Volume 35, Issues 6–7, 2004<o:p></o:p></span></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td style="border:solid #AAAAAA 1.0pt;border-top:none;mso-border-top-alt:
 +
  solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:14.25pt;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">[8]<o:p></o:p></span></p>
 +
  </td>
 +
  <td style="border-top:none;border-left:none;border-bottom:solid #AAAAAA 1.0pt;
 +
  border-right:solid #AAAAAA 1.0pt;mso-border-top-alt:solid #AAAAAA .75pt;
 +
  mso-border-left-alt:solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;
 +
  padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" style="line-height:14.25pt;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;;
 +
  mso-fareast-font-family:新細明體;mso-font-kerning:0pt">Bengt Borgstrom,<b> Luminal Digestion of Fats,</b> Handbook
 +
  of Physiology, The Gastrointestinal System, Intestinal Absorption and
 +
  Secretion</span>, 1991<o:p></o:p></p>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td style="border:solid #AAAAAA 1.0pt;border-top:none;mso-border-top-alt:
 +
  solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" align="center" style="text-align:center;line-height:14.25pt;
 +
  mso-pagination:widow-orphan"><span lang="EN-US" style="font-size: 10pt; font-family: Arial, sans-serif; ">[9]<o:p></o:p></span></p>
 +
  </td>
 +
  <td style="border-top:none;border-left:none;border-bottom:solid #AAAAAA 1.0pt;
 +
  border-right:solid #AAAAAA 1.0pt;mso-border-top-alt:solid #AAAAAA .75pt;
 +
  mso-border-left-alt:solid #AAAAAA .75pt;mso-border-alt:solid #AAAAAA .75pt;
 +
  padding:2.4pt 2.4pt 2.4pt 2.4pt">
 +
  <p class="MsoNormal" style="line-height:14.25pt;mso-pagination:widow-orphan"><span lang="EN-US" style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;;
 +
  mso-fareast-font-family:新細明體;mso-font-kerning:0pt">Sallee VL,</span><span lang="EN-US" style="font-size:10.0pt;mso-fareast-font-family:新細明體;mso-font-kerning:
 +
  0pt">&nbsp;</span><span lang="EN-US" style="font-size:10.0pt;font-family:&quot;Arial&quot;,&quot;sans-serif&quot;;
 +
  mso-fareast-font-family:新細明體;mso-font-kerning:0pt">Dietschy JM.</span>, <b>Determinants of intestinal mucosal uptake
 +
  of short- and medium-chain fatty acids and alcohols, </b>Journal of Lipid
 +
  Research, 1973<o:p></o:p></p>
 +
  </td>
 +
</tr>
 +
</tbody></table>
 +
</html>

Revision as of 02:05, 27 September 2012

Contents

Single-Cell/ System analysis Model

Parameter

Description 

Value 

Reference

cd

E. coli Cell Density

0.1

 

DFA

FA Diffusion Constant

6.46* 10^-10m^2 /s

DAHL

AHL Diffusion Constant

4.9e-6 cm2/s

[9]

γAHL,ext

AHL cell-external degradation

8.0225e-006/s

Derived from 1 day half-life at pH 7 <a href="http://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref7" title="Team:ETH Zurich/Modeling/References">[7]</a>

Fatty Acid Reaction Absorption Model Model

Parameter

Description 

Value 

Reference

[E]t

Total active enzyme

50 uM

[8]

Km

Reaction rate constant

47.9

[6][7]

DFA

FA Diffusion Constant

6.46* <!--[if gte msEquation 12]><m:oMath><m:sSup><m:sSupPr><m:ctrlPr></m:ctrlPr></m:sSupPr><m:e><m:r><m:rPr><m:scr m:val="roman"/><m:sty m:val="p"/></m:rPr>10</m:r></m:e><m:sup><m:r><m:rPr><m:scr m:val="roman"/><m:sty m:val="p"/></m:rPr>-</m:r><m:r><m:rPr><m:scr

m:val="roman"/><m:sty m:val="p"/></m:rPr>10</m:r></m:sup></m:sSup><m:sSup><m:sSupPr><m:ctrlPr></m:ctrlPr></m:sSupPr><m:e><m:r><m:rPr><m:scr m:val="roman"/><m:sty m:val="p"/></m:rPr>m</m:r></m:e><m:sup><m:r><m:rPr><m:scr m:val="roman"/><m:sty m:val="p"/></m:rPr>2</m:r></m:sup></m:sSup><m:r><m:rPr><m:scr m:val="roman"/><m:sty m:val="p"/></m:rPr> /s</m:r></m:oMath><![endif]--><!--[if !msEquation]--><!--[if gte vml 1]><v:shapetype id="_x0000_t75" coordsize="21600,21600" o:spt="75" o:preferrelative="t" path="m@4@5l@4@11@9@11@9@5xe" filled="f" stroked="f"><v:stroke joinstyle="miter"/><v:formulas><v:f eqn="if lineDrawn pixelLineWidth 0"/><v:f eqn="sum @0 1 0"/><v:f eqn="sum 0 0 @1"/><v:f eqn="prod @2 1 2"/><v:f eqn="prod @3 21600 pixelWidth"/><v:f eqn="prod @3 21600 pixelHeight"/><v:f eqn="sum @0 0 1"/><v:f eqn="prod @6 1 2"/><v:f eqn="prod @7 21600 pixelWidth"/><v:f eqn="sum @8 21600 0"/><v:f eqn="prod @7 21600 pixelHeight"/><v:f eqn="sum @10 21600 0"/></v:formulas><v:path o:extrusionok="f" gradientshapeok="t" o:connecttype="rect"/><o:lock v:ext="edit" aspectratio="t"/></v:shapetype><v:shape id="_x0000_i1025" type="#_x0000_t75" style='width:48.75pt; height:12pt'><v:imagedata src="file:///C:\Users\curtis\AppData\Local\Temp\msohtmlclip1\01\clip_image001.png"

o:title="" chromakey="white"/></v:shape><![endif]--><!--[if !vml]--><img width="65" height="16" src="file:///C:/Users/curtis/AppData/Local/Temp/msohtmlclip1/01/clip_image002.png" v:shapes="_x0000_i1025"><!--[endif]--><!--[endif]-->

 

d

Thickness of the unstirred water layer

190 um

[9]

Kcat/ molecular weight

Catalytic Rate constant

1.8*10^-3

[6][7]

Cell-Cell Communication Model

Parameter

Description 

Value 

Reference

cd

E. coli Cell Density

0.1

 

DFA

FA Diffusion Constant

6.46* 10^-10m^2 /s

DAHL

AHL Diffusion Constant

4.9e-6 cm2/s

[9]

γAHL,ext

AHL cell-external degradation

8.0225e-006/s

Derived from 1 day half-life at pH 7 <a href="http://2011.igem.org/Team:ETH_Zurich/Modeling/References#Ref7" title="Team:ETH Zurich/Modeling/References">[7]</a>



Reference

Source

[1]

Subhayu Basu, Yoram Gerchman, Cynthia H. Collins, Frances H. Arnold & Ron Weiss.A synthetic multicellular system for programmed pattern formation, Nature Vol. 434, 2005

[2]

Garcia-Ojalvo, Michael B. Elowitz, and Steven H. Strogatz. Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing, PNAS vol. 101 no. 30, 2004

[3]

MIT igem 2010

[4]

Michael Halter, Alex Tona, Kiran Bhadriraju, Anne L. Plant, John T. Elliott, Automated Live Cell Imaging of Green Fluorescent Protein Degradation in Individual Fibroblasts, Cytometry Part A, Volume 71A Issue 10, 2007

[5]

Wilfried Weber, Markus Rimann, Manuela Spielmann, Bettina Keller, Marie Daoud-El Baba, Dominique Aubel, Cornelia C Weber & Martin Fussenegger, Gas-inducible transgene expression in mammalian cells and mice, Nature Biotechnology, volume 22, number 11, 2004

[6]

Sulaiman Al-Zuhair, Masitah Hasan, K.B. Ramachandran, Kinetics of the enzymatic hydrolysis of palm oil by lipase, Process Biochemistry

Volume 38, Issue 8, 2003

[7]

Ho-Shing Wu, Ming-Ju Tsai, Kinetics of tributyrin hydrolysis by lipase, Enzyme and Microbial Technology, Volume 35, Issues 6–7, 2004

[8]

Bengt Borgstrom, Luminal Digestion of Fats, Handbook of Physiology, The Gastrointestinal System, Intestinal Absorption and Secretion, 1991

[9]

Sallee VL, Dietschy JM., Determinants of intestinal mucosal uptake of short- and medium-chain fatty acids and alcohols, Journal of Lipid Research, 1973