"
Team:NTNU Trondheim/Stocks
From 2012.igem.org
(Difference between revisions)
(→Isolated DNA) |
(→Isolated DNA) |
||
| Line 32: | Line 32: | ||
{| class="wikitable" | {| class="wikitable" | ||
! Name/abbreviation || Date stored || Description || Concentration|| Backbone | ! Name/abbreviation || Date stored || Description || Concentration|| Backbone | ||
| + | |- | ||
| + | |pTet GFP||26.07||<partinfo>BBa_I13522</partinfo>|| ||<partinfo>pSB1A2</partinfo> | ||
|- | |- | ||
|VGB+RBS+YFP+DTT||26.07|| || || | |VGB+RBS+YFP+DTT||26.07|| || || | ||
| Line 42: | Line 44: | ||
|- | |- | ||
|LuxI+Term||16.7||<partinfo>BBa_C0061</partinfo> + <partinfo>BBa_B0015</partinfo>||37,6 ||<partinfo>pSB1AK3</partinfo> | |LuxI+Term||16.7||<partinfo>BBa_C0061</partinfo> + <partinfo>BBa_B0015</partinfo>||37,6 ||<partinfo>pSB1AK3</partinfo> | ||
| + | |- | ||
| + | |LI (BBa_K092400)||?||<partinfo>BBa_K092400</partinfo>|| ||<partinfo>pSB1A2</partinfo> | ||
|- | |- | ||
|GFP+DTT||13.07||<partinfo>BBa_K082003</partinfo>+<partinfo>BBa_B0015</partinfo>||41,1||<partinfo>pSB1AK3</partinfo> | |GFP+DTT||13.07||<partinfo>BBa_K082003</partinfo>+<partinfo>BBa_B0015</partinfo>||41,1||<partinfo>pSB1AK3</partinfo> | ||
| Line 51: | Line 55: | ||
|CFP+DTT||13.07||<partinfo>BBa_E0020</partinfo>+<partinfo>BBa_B0015</partinfo>||47,7||<partinfo>pSB1AK3</partinfo> | |CFP+DTT||13.07||<partinfo>BBa_E0020</partinfo>+<partinfo>BBa_B0015</partinfo>||47,7||<partinfo>pSB1AK3</partinfo> | ||
|- | |- | ||
| - | |||
| - | |||
| - | |||
|- | |- | ||
| + | |K+RBS||13.07||<partinfo>BBa_K081005</partinfo>||19,8||<partinfo>pSB1A2</partinfo> | ||
|DLD + RBS||9.07||<partinfo>BBa_K284003</partinfo> + <partinfo>BBa_B0034</partinfo> ||24,1||<partinfo>pSB1A2</partinfo> | |DLD + RBS||9.07||<partinfo>BBa_K284003</partinfo> + <partinfo>BBa_B0034</partinfo> ||24,1||<partinfo>pSB1A2</partinfo> | ||
|- | |- | ||
| Line 68: | Line 70: | ||
|- | |- | ||
|LuxI||6.07||<partinfo>BBa_C0061</partinfo> ||38,6||<partinfo>pSB1A2</partinfo> | |LuxI||6.07||<partinfo>BBa_C0061</partinfo> ||38,6||<partinfo>pSB1A2</partinfo> | ||
| + | |- | ||
| + | |Colicin||4.07||<partinfo>BBa_K150009</partinfo>||58,6||<partinfo>pSB1A2</partinfo> | ||
|- | |- | ||
|VHB||4.07||<partinfo>BBa_K258005</partinfo>||44,9||<partinfo>pSB1A2</partinfo> | |VHB||4.07||<partinfo>BBa_K258005</partinfo>||44,9||<partinfo>pSB1A2</partinfo> | ||
| Line 74: | Line 78: | ||
|- | |- | ||
|DLD||4.07||<partinfo>BBa_K284003</partinfo>||63,8||<partinfo>BBa_J63010</partinfo> | |DLD||4.07||<partinfo>BBa_K284003</partinfo>||63,8||<partinfo>BBa_J63010</partinfo> | ||
| - | |||
| - | |||
|- | |- | ||
|pBAD B1||1.07||<partinfo>BBa_K206000</partinfo>||24,0||<partinfo>pSB1A3</partinfo> | |pBAD B1||1.07||<partinfo>BBa_K206000</partinfo>||24,0||<partinfo>pSB1A3</partinfo> | ||
|- | |- | ||
|pBAD B2||1.07||<partinfo>Bba_K206000</partinfo>||19||<partinfo>pSB1A3</partinfo> | |pBAD B2||1.07||<partinfo>Bba_K206000</partinfo>||19||<partinfo>pSB1A3</partinfo> | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
|- | |- | ||
|LR||?||<partinfo>BBa_C0062</partinfo> ||20,3||<partinfo>pSB1A2</partinfo> | |LR||?||<partinfo>BBa_C0062</partinfo> ||20,3||<partinfo>pSB1A2</partinfo> | ||
| Line 91: | Line 87: | ||
|VGB promoter||27.06||<partinfo>BBa_K561001</partinfo>||22,9||<partinfo>pSB1C3</partinfo> | |VGB promoter||27.06||<partinfo>BBa_K561001</partinfo>||22,9||<partinfo>pSB1C3</partinfo> | ||
|- | |- | ||
| - | | | + | |C79||27.06||<partinfo>BBa_C0079</partinfo>||70,8||<partinfo>pSB2K3</partinfo> |
|- | |- | ||
| - | | | + | |C78||27.06||<partinfo>BBa_C0078</partinfo>||63,4||<partinfo>pSB2K3</partinfo> |
|- | |- | ||
| - | | | + | |RBS||27.06||<partinfo>BBa_B0034</partinfo>||30,2||<partinfo>pSB1A2</partinfo> |
|- | |- | ||
| - | | | + | |VGB DNA||25.06||<partinfo>BBa_K561001</partinfo>|| ||<partinfo>pSB1C3</partinfo> |
|- | |- | ||
|luxR+HSL||25.06||<partinfo>BBa_R0062</partinfo> ||13,3||<partinfo>pSB1A2</partinfo> | |luxR+HSL||25.06||<partinfo>BBa_R0062</partinfo> ||13,3||<partinfo>pSB1A2</partinfo> | ||
| - | |||
| - | |||
|- | |- | ||
|Lysis1 DNA||24.06||<partinfo>BBa_K112808</partinfo> || ||<partinfo>pSB1A2</partinfo> | |Lysis1 DNA||24.06||<partinfo>BBa_K112808</partinfo> || ||<partinfo>pSB1A2</partinfo> | ||
| Line 114: | Line 108: | ||
|- | |- | ||
|D1||22.06||<partinfo>BBa_K091146</partinfo>||19,8||<partinfo>pSB1A2</partinfo> | |D1||22.06||<partinfo>BBa_K091146</partinfo>||19,8||<partinfo>pSB1A2</partinfo> | ||
| + | |- | ||
| + | |RBS||26.06||<partinfo>BBa_B0034</partinfo>||16,8||<partinfo>pSB1A2</partinfo> | ||
| + | |- | ||
| + | |DTT Amp||26.06||<partinfo>BBa_B0015</partinfo>||35,8||<partinfo>pSB1AK3</partinfo> | ||
| + | |- | ||
| + | |DTT Kan||26.06||<partinfo>BBa_B0015</partinfo>||26,5||<partinfo>pSB1AK3</partinfo> | ||
|- | |- | ||
|RFP K||22.06||<partinfo>BBa_E1010</partinfo> || ||<partinfo>pSB2K3</partinfo> | |RFP K||22.06||<partinfo>BBa_E1010</partinfo> || ||<partinfo>pSB2K3</partinfo> | ||
Revision as of 20:40, 28 July 2012
This page lists stored cultures and other samples:
See also Constructs page.
Contents |
Stored samples
Resuspended DNA
(Dried DNA from distribution kit resuspended in dH2O)
| Name/abbreviation | Date stored | Description | Source | Backbone |
|---|---|---|---|---|
| LR | ? | <partinfo>BBa_C0062</partinfo> | (RS) | <partinfo>pSB1A2</partinfo> |
| Lysis | 22.06 | BBa_K112808 | (RS) | <partinfo>pSB1A2</partinfo> |
| C78 | ? | <partinfo>BBa_C0078</partinfo> | (RS) | <partinfo>pSB2K3</partinfo> |
| C79 | ? | <partinfo>BBa_C0079</partinfo> | (RS) | <partinfo>pSB2K3</partinfo> |
| RBS | 24.06 | <partinfo>BBa_B0034</partinfo> | (RS) | <partinfo>pSB1A2</partinfo> |
| LuxI | ? | <partinfo>BBa_C0061</partinfo> | (RS) | <partinfo>pSB1A2</partinfo> |
| K+RBS | ? | <partinfo>BBa_K081005</partinfo> | (RS) | <partinfo>pSB1A2</partinfo> |
Isolated DNA
(Miniprep, gel extraction and PCR products) NOT included here: Digestion and ligation mixtures/products
| Name/abbreviation | Date stored | Description | Concentration | Backbone | |||||
|---|---|---|---|---|---|---|---|---|---|
| pTet GFP | 26.07 | <partinfo>BBa_I13522</partinfo> | <partinfo>pSB1A2</partinfo> | ||||||
| VGB+RBS+YFP+DTT | 26.07 | ||||||||
| VHB+RBS+YFP+DTT | 26.07 | ||||||||
| EYFP+RBS+YFP+DTT | 26.07 | ||||||||
| LuxR+Term | 16.7 | <partinfo>BBa_C0062</partinfo> + <partinfo>BBa_B0015</partinfo> | 37,0 | <partinfo>pSB1AK3</partinfo> | |||||
| LuxI+Term | 16.7 | <partinfo>BBa_C0061</partinfo> + <partinfo>BBa_B0015</partinfo> | 37,6 | <partinfo>pSB1AK3</partinfo> | |||||
| LI (BBa_K092400) | ? | <partinfo>BBa_K092400</partinfo> | <partinfo>pSB1A2</partinfo> | ||||||
| GFP+DTT | 13.07 | <partinfo>BBa_K082003</partinfo>+<partinfo>BBa_B0015</partinfo> | 41,1 | <partinfo>pSB1AK3</partinfo> | |||||
| YFP+DTT | 13.07 | <partinfo>BBa_E0030</partinfo>+<partinfo>BBa_B0015</partinfo> | 55,5 | <partinfo>pSB1AK3</partinfo> | |||||
| RFP+DTT | 13.07 | <partinfo>BBa_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> | 40,9 | <partinfo>pSB1AK3</partinfo> | |||||
| CFP+DTT | 13.07 | <partinfo>BBa_E0020</partinfo>+<partinfo>BBa_B0015</partinfo> | 47,7 | <partinfo>pSB1AK3</partinfo> | |||||
| K+RBS | 13.07 | <partinfo>BBa_K081005</partinfo> | 19,8 | <partinfo>pSB1A2</partinfo> | DLD + RBS | 9.07 | <partinfo>BBa_K284003</partinfo> + <partinfo>BBa_B0034</partinfo> | 24,1 | <partinfo>pSB1A2</partinfo> |
| VHB + RBS | 9.07 | <partinfo>BBa_K258005</partinfo> + <partinfo>BBa_B0034</partinfo> | 48,3 | <partinfo>pSB1A2</partinfo> | |||||
| Jen1 + RBS | 9.07 | <partinfo>BBa_K284002</partinfo> + <partinfo>BBa_B0034</partinfo> | 35,3 | <partinfo>pSB1A2</partinfo> | |||||
| VGB + RBS | 9.07 | <partinfo>BBa_K561001</partinfo> + <partinfo>BBa_B0034</partinfo> | 40,0 | <partinfo>pSB1A2</partinfo> | |||||
| pBAD+lysis | 9.7 | 23,6 | <partinfo>pSB1A3</partinfo> | ||||||
| RBS | 6.07 | <partinfo>BBa_B0034</partinfo> | 44,5 | <partinfo>pSB1A2</partinfo> | |||||
| LuxI | 6.07 | <partinfo>BBa_C0061</partinfo> | 38,6 | <partinfo>pSB1A2</partinfo> | |||||
| Colicin | 4.07 | <partinfo>BBa_K150009</partinfo> | 58,6 | <partinfo>pSB1A2</partinfo> | |||||
| VHB | 4.07 | <partinfo>BBa_K258005</partinfo> | 44,9 | <partinfo>pSB1A2</partinfo> | |||||
| Jen1 | 4.07 | <partinfo>BBa_K284002</partinfo> | 102,1 | <partinfo>BBa_J63010</partinfo> | |||||
| DLD | 4.07 | <partinfo>BBa_K284003</partinfo> | 63,8 | <partinfo>BBa_J63010</partinfo> | |||||
| pBAD B1 | 1.07 | <partinfo>BBa_K206000</partinfo> | 24,0 | <partinfo>pSB1A3</partinfo> | |||||
| pBAD B2 | 1.07 | <partinfo>Bba_K206000</partinfo> | 19 | <partinfo>pSB1A3</partinfo> | |||||
| LR | ? | <partinfo>BBa_C0062</partinfo> | 20,3 | <partinfo>pSB1A2</partinfo> | |||||
| VGB promoter | 27.06 | <partinfo>BBa_K561001</partinfo> | 22,9 | <partinfo>pSB1C3</partinfo> | |||||
| C79 | 27.06 | <partinfo>BBa_C0079</partinfo> | 70,8 | <partinfo>pSB2K3</partinfo> | |||||
| C78 | 27.06 | <partinfo>BBa_C0078</partinfo> | 63,4 | <partinfo>pSB2K3</partinfo> | |||||
| RBS | 27.06 | <partinfo>BBa_B0034</partinfo> | 30,2 | <partinfo>pSB1A2</partinfo> | |||||
| VGB DNA | 25.06 | <partinfo>BBa_K561001</partinfo> | <partinfo>pSB1C3</partinfo> | ||||||
| luxR+HSL | 25.06 | <partinfo>BBa_R0062</partinfo> | 13,3 | <partinfo>pSB1A2</partinfo> | |||||
| Lysis1 DNA | 24.06 | <partinfo>BBa_K112808</partinfo> | <partinfo>pSB1A2</partinfo> | ||||||
| LuxR+HSL | 24.06 | <partinfo>BBa_R0062</partinfo> | <partinfo>pSB1A2</partinfo> | ||||||
| DNA lysis | 24.06 | <partinfo>BBa_K112808</partinfo> | <partinfo>pSB1A2</partinfo> | ||||||
| Constitutive promoter | 22.06 | <partinfo>BBa_J23119</partinfo> | 27,7 | <partinfo>pSB1A2</partinfo> | |||||
| D2 | 22.6 | <partinfo>BBa_K145150</partinfo> | 23,1 | <partinfo>pSB1A2</partinfo> | |||||
| D1 | 22.06 | <partinfo>BBa_K091146</partinfo> | 19,8 | <partinfo>pSB1A2</partinfo> | |||||
| RBS | 26.06 | <partinfo>BBa_B0034</partinfo> | 16,8 | <partinfo>pSB1A2</partinfo> | |||||
| DTT Amp | 26.06 | <partinfo>BBa_B0015</partinfo> | 35,8 | <partinfo>pSB1AK3</partinfo> | |||||
| DTT Kan | 26.06 | <partinfo>BBa_B0015</partinfo> | 26,5 | <partinfo>pSB1AK3</partinfo> | |||||
| RFP K | 22.06 | <partinfo>BBa_E1010</partinfo> | <partinfo>pSB2K3</partinfo> | ||||||
| GFP AK | 22.06 | <partinfo>BBa_K082003</partinfo> | <partinfo>pSB1AK3</partinfo> | ||||||
| CFP A | 22.06 | <partinfo>BBa_E0020</partinfo> | <partinfo>pSB1A2</partinfo> | ||||||
| YFP AK | 22.06 | <partinfo>BBa_E0030</partinfo> | <partinfo>pSB1AK3</partinfo> |
Restriction digests and ligation mixtures
(Inserts and backbones, DNA treated with enzymes)
| Name/abbreviation | Date stored | Description | Enzymes | |
|---|---|---|---|---|
| YFP+Term (religation) | 11.07 | YFP+term (religated) | ? | <partinfo>pSB1AK3</partinfo> |
| GFP+Term (religation) | 11.07 | <partinfo>BBa_K082003</partinfo> + <partinfo>BBa_B0015</partinfo> | <partinfo>pSB1AK3</partinfo> | |
| CFP+Term (religation) | 11.07 | <partinfo>BBa_E0020</partinfo> + <partinfo>BBa_B0015</partinfo> | <partinfo>pSB1AK3</partinfo> | |
| RFP +Term (religation) | 11.07 | <partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> | <partinfo>pSB1AK3</partinfo> |
Glycerol stocks
(Cell cultures kept at -80 C)
| Name/abbreviation | Date stored | Strain | Plasmid | Position |
|---|---|---|---|---|
| pTet mRFP (BBa_I13521) | 27.07 | DH5a | pSB1A2 | |
| pTet GFP (BBa_I13522) | 27.07 | DH5a | pSB1A2 | |
| EYFP + LVA (BBa_E0430) | 27.07 | DH5a | pSB1A2 | |
| RFP (<partinfo>BBa_E1010</partinfo>) | 18.07 | DH5a | pSB2K3 | |
| VGB (<partinfo>BBa_K561001</partinfo>) | 18.07 | DH5a | pSB1C3 | |
| LuxR+HSL (<partinfo>BBa_R0062</partinfo>) | 16.07 | DH5a | pSB1A2 | |
| Lysis (<partinfo>BBa_K112808</partinfo>) | 16.07 | DH5a | pSB1A2 | |
| pBAD (<partinfo>Bba_K206000</partinfo>) | 16.07 | DH5a | pSB1A3 | |
| LuxR (<partinfo>BBa_C0062</partinfo>) | 16.07 | DH5a | pSB1A2 | |
| GFP (<partinfo>BBa_K082003</partinfo>) | 16.07 | DH5a | pSB1AK3 | |
| pBAD+Lysis | 16.07 | DH5a | pSB1A3 | |
| DTT (<partinfo>BBa_B0015</partinfo> | 17.07 | DH5a | pSB1AK3 | |
| D2 (<partinfo>BBa_K145150</partinfo>) | 17.07 | DH5a | pSB1A2 | |
| CFP (<partinfo>BBa_E0020</partinfo>) | 17.07 | DH5a | pSB1A2 | |
| YFP (<partinfo>BBa_E0030</partinfo>) | 17.07 | DH5a | pSB1AK3 |
