Team:NTNU Trondheim/Stocks

From 2012.igem.org

(Difference between revisions)
(→Restriction digests and ligation mixtures)
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See also [https://2012.igem.org/Team:NTNU_Trondheim/Constructs Constructs page].
See also [https://2012.igem.org/Team:NTNU_Trondheim/Constructs Constructs page].
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==Stored samples==
===Resuspended DNA===
===Resuspended DNA===
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|VGB DNA||25.06||<partinfo>BBa_K561001</partinfo>|| ||<partinfo>pSB1C3</partinfo>
|VGB DNA||25.06||<partinfo>BBa_K561001</partinfo>|| ||<partinfo>pSB1C3</partinfo>
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|RFP +Term||11.07||<partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> || ||<partinfo>pSB1AK3</partinfo>
 
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|Constitutive promoter||22.06||<partinfo>BBa_J23119</partinfo> ||27,7||<partinfo>pSB1A2</partinfo>
|Constitutive promoter||22.06||<partinfo>BBa_J23119</partinfo> ||27,7||<partinfo>pSB1A2</partinfo>
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! Name/abbreviation ||Date stored|| Description ||Enzymes||  
! Name/abbreviation ||Date stored|| Description ||Enzymes||  
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|YFP+Term||11.07||?|YFP+term (religated)||?
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|YFP+Term (religation)||11.07||?|YFP+term (religated)||?
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|GFP+Term (religation)||11.07||<partinfo>BBa_K082003</partinfo> + <partinfo>BBa_B0015</partinfo>|| ||<partinfo>pSB1AK3</partinfo>
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|GFP+Term||11.07||<partinfo>BBa_K082003</partinfo> + <partinfo>BBa_B0015</partinfo>|| ||<partinfo>pSB1AK3</partinfo>
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|CFP+Term (religation)||11.07||<partinfo>BBa_E0020</partinfo> + <partinfo>BBa_B0015</partinfo> || ||<partinfo>pSB1AK3</partinfo>
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|CFP+Term||11.07||<partinfo>BBa_E0020</partinfo> + <partinfo>BBa_B0015</partinfo> || ||<partinfo>pSB1AK3</partinfo>
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|RFP +Term (religation)||11.07||<partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> || ||<partinfo>pSB1AK3</partinfo>
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Revision as of 20:06, 28 July 2012

This page lists stored cultures and other samples:

See also Constructs page.

Contents

Stored samples

Resuspended DNA

(Dried DNA from distribution kit resuspended in dH2O)

Name/abbreviation Date stored Description Source Backbone
LR? <partinfo>BBa_C0062</partinfo>(RS)<partinfo>pSB1A2</partinfo>
Lysis22.06 BBa_K112808(RS)<partinfo>pSB1A2</partinfo>
C78?<partinfo>BBa_C0078</partinfo>(RS)<partinfo>pSB2K3</partinfo>
C79?<partinfo>BBa_C0079</partinfo>(RS)<partinfo>pSB2K3</partinfo>
RBS24.06<partinfo>BBa_B0034</partinfo>(RS)<partinfo>pSB1A2</partinfo>
LuxI?<partinfo>BBa_C0061</partinfo>(RS)<partinfo>pSB1A2</partinfo>
K+RBS?<partinfo>BBa_K081005</partinfo>(RS)<partinfo>pSB1A2</partinfo>

Isolated DNA

(Miniprep, gel extraction and PCR products) NOT included here: Digestion and ligation mixtures/products

Name/abbreviation Date stored Description Concentration Backbone
LuxR+Term16.7<partinfo>BBa_C0062</partinfo> + <partinfo>BBa_B0015</partinfo> 37,0 <partinfo>pSB1AK3</partinfo>
LuxI+Term16.7<partinfo>BBa_C0061</partinfo> + <partinfo>BBa_B0015</partinfo>37,6 <partinfo>pSB1AK3</partinfo>
C7927.06<partinfo>BBa_C0079</partinfo>70,8<partinfo>pSB2K3</partinfo>
C7827.06<partinfo>BBa_C0078</partinfo>63,4<partinfo>pSB2K3</partinfo>
RBS27.06<partinfo>BBa_B0034</partinfo>30,2<partinfo>pSB1A2</partinfo>
LI (BBa_K092400)?<partinfo>BBa_K092400</partinfo> <partinfo>pSB1A2</partinfo>
DLD + RBS9.07<partinfo>BBa_K284003</partinfo> + <partinfo>BBa_B0034</partinfo> 24,1<partinfo>pSB1A2</partinfo>
VHB + RBS9.07<partinfo>BBa_K258005</partinfo> + <partinfo>BBa_B0034</partinfo> 48,3<partinfo>pSB1A2</partinfo>
Jen1 + RBS9.07<partinfo>BBa_K284002</partinfo> + <partinfo>BBa_B0034</partinfo> 35,3<partinfo>pSB1A2</partinfo>
VGB + RBS9.07<partinfo>BBa_K561001</partinfo> + <partinfo>BBa_B0034</partinfo> 40,0<partinfo>pSB1A2</partinfo>
LR?<partinfo>BBa_C0062</partinfo> 20,3<partinfo>pSB1A2</partinfo>
VGB promoter27.06<partinfo>BBa_K561001</partinfo>22,9<partinfo>pSB1C3</partinfo>
VGB DNA25.06<partinfo>BBa_K561001</partinfo> <partinfo>pSB1C3</partinfo>
Constitutive promoter22.06<partinfo>BBa_J23119</partinfo> 27,7<partinfo>pSB1A2</partinfo>
DLD4.07<partinfo>BBa_K284003</partinfo>63,8<partinfo>BBa_J63010</partinfo>
K+RBS13.07<partinfo>BBa_K081005</partinfo>19,8<partinfo>pSB1A2</partinfo>
LuxR+HSL24.06<partinfo>BBa_R0062</partinfo> <partinfo>pSB1A2</partinfo>
pBAD+lysis9.7 23,6<partinfo>pSB1A3</partinfo>
DNA lysis24.06<partinfo>BBa_K112808</partinfo> <partinfo>pSB1A2</partinfo>
D222.6<partinfo>BBa_K145150</partinfo>23,1<partinfo>pSB1A2</partinfo>
RBS6.07<partinfo>BBa_B0034</partinfo>44,5<partinfo>pSB1A2</partinfo>
LuxI6.07<partinfo>BBa_C0061</partinfo> 38,6<partinfo>pSB1A2</partinfo>
VHB4.07<partinfo>BBa_K258005</partinfo>44,9<partinfo>pSB1A2</partinfo>
Jen14.07<partinfo>BBa_K284002</partinfo>102,1<partinfo>BBa_J63010</partinfo>
luxR+HSL25.06<partinfo>BBa_R0062</partinfo> 13,3<partinfo>pSB1A2</partinfo>
Lysis1 DNA24.06<partinfo>BBa_K112808</partinfo> <partinfo>pSB1A2</partinfo>
GFP+DTT13.07<partinfo>BBa_K082003</partinfo>+<partinfo>BBa_B0015</partinfo>41,1<partinfo>pSB1AK3</partinfo>
YFP+DTT13.07<partinfo>BBa_E0030</partinfo>+<partinfo>BBa_B0015</partinfo>55,5<partinfo>pSB1AK3</partinfo>
RFP+DTT13.07<partinfo>BBa_E1010</partinfo>+<partinfo>BBa_B0015</partinfo>40,9<partinfo>pSB1AK3</partinfo>
CFP+DTT13.07<partinfo>BBa_E0020</partinfo>+<partinfo>BBa_B0015</partinfo>47,7<partinfo>pSB1AK3</partinfo>
pBAD B11.07<partinfo>BBa_K206000</partinfo>24,0<partinfo>pSB1A3</partinfo>
D122.06<partinfo>BBa_K091146</partinfo>19,8<partinfo>pSB1A2</partinfo>
pBAD B21.07<partinfo>Bba_K206000</partinfo>19<partinfo>pSB1A3</partinfo>
Colicin4.07<partinfo>BBa_K150009</partinfo>58,6<partinfo>pSB1A2</partinfo>
VGB+RBS+YFP+DTT26.07
VHB+RBS+YFP+DTT26.07
EYFP+RBS+YFP+DTT26.07
pTet GFP26.07<partinfo>BBa_I13522</partinfo> <partinfo>pSB1A2</partinfo>
RBS26.06<partinfo>BBa_B0034</partinfo>16,8<partinfo>pSB1A2</partinfo>
DTT Amp26.06<partinfo>BBa_B0015</partinfo>35,8<partinfo>pSB1AK3</partinfo>
DTT Kan26.06<partinfo>BBa_B0015</partinfo>26,5<partinfo>pSB1AK3</partinfo>
RFP K22.06<partinfo>BBa_E1010</partinfo> <partinfo>pSB2K3</partinfo>
GFP AK22.06<partinfo>BBa_K082003</partinfo> <partinfo>pSB1AK3</partinfo>
CFP A22.06<partinfo>BBa_E0020</partinfo> <partinfo>pSB1A2</partinfo>
YFP AK22.06 <partinfo>BBa_E0030</partinfo> <partinfo>pSB1AK3</partinfo>

Restriction digests and ligation mixtures

(Inserts and backbones, DNA treated with enzymes)


Name/abbreviation Date stored Description Enzymes
YFP+Term (religation)11.07YFP+term (religated)?
GFP+Term (religation)11.07<partinfo>BBa_K082003</partinfo> + <partinfo>BBa_B0015</partinfo> <partinfo>pSB1AK3</partinfo>
CFP+Term (religation)11.07<partinfo>BBa_E0020</partinfo> + <partinfo>BBa_B0015</partinfo> <partinfo>pSB1AK3</partinfo>
RFP +Term (religation)11.07<partinfo>Bba_E1010</partinfo>+<partinfo>BBa_B0015</partinfo> <partinfo>pSB1AK3</partinfo>

Glycerol stocks

(Cell cultures kept at -80 C)

Name/abbreviation Date stored Strain Plasmid Position
pTet mRFP (BBa_I13521)27.07DH5apSB1A2
pTet GFP (BBa_I13522)27.07DH5apSB1A2
EYFP + LVA (BBa_E0430)27.07DH5apSB1A2
RFP (<partinfo>BBa_E1010</partinfo>) 18.07 DH5a pSB2K3
VGB (<partinfo>BBa_K561001</partinfo>)18.07DH5apSB1C3
LuxR+HSL (<partinfo>BBa_R0062</partinfo>)16.07 DH5apSB1A2
Lysis (<partinfo>BBa_K112808</partinfo>)16.07 DH5apSB1A2
pBAD (<partinfo>Bba_K206000</partinfo>)16.07 DH5apSB1A3
LuxR (<partinfo>BBa_C0062</partinfo>)16.07 DH5apSB1A2
GFP (<partinfo>BBa_K082003</partinfo>)16.07 DH5apSB1AK3
pBAD+Lysis16.07 DH5apSB1A3
DTT (<partinfo>BBa_B0015</partinfo>17.07 DH5apSB1AK3
D2 (<partinfo>BBa_K145150</partinfo>)17.07 DH5apSB1A2
CFP (<partinfo>BBa_E0020</partinfo>)17.07 DH5apSB1A2
YFP (<partinfo>BBa_E0030</partinfo>)17.07 DH5apSB1AK3
Retrieved from "http://2012.igem.org/Team:NTNU_Trondheim/Stocks"