Team:NRP-UEA-Norwich/Week6

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NRP UEA iGEM 2012

 

Welcome to the NRP UEA iGEM 2012 Wiki Lab Book

Please choose the relevant link to access our diary of that week!

Week Zero | Week One | Week Two | Week Three | Week Four | Week Five | Week Six | Week Seven | Week Eight | Week Nine | Week Ten | Week Eleven | Lab Protocols | Experiments

Contents

Week 6

Day 1

. We have BioBricks! - Practice run of growth study with absorbance readings only. Contamination. Lasted 6 hours.

Day 2

- Rebecca did 9 hour growth study with wavescan function -Over 50 plates to generate more cells.

Day 3

- Forum preparation - plates with Biobrick written with one letter per plate (using the pYEAR with GFP and potassium nitrate to induce promoter activity) - Rebecca, Khadija and Luas, used day 2 samples and carried out a growth study with a reading every minute for 1 hour.

Day 4

- Forum preparation - plates used for the forum science day had their fluorescence intensified by adding more potassium nitrate to the cells on the plate.

Day 5

. UK Team Meet-Up!