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Team:Macquarie Australia/ProjectOverview
From 2012.igem.org
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<a name="1"><h3><li>Design of Heme oxygenase and two bacteriophytochrome fragments </h3> | <a name="1"><h3><li>Design of Heme oxygenase and two bacteriophytochrome fragments </h3> | ||
| - | <p> | + | <p>We selected three genes for our project, a single heme oxygenase and bacteriophytochromes from <i>Deinococcus radiodurans</i> and <i>Agrobacterium tumefaciens</i>. They were designed to remove any internal restriction sites without the need for site directed mutagenesis. The genes will also be codon optimised for <i>E. coli</i>. In this way a heme oxygenase gene will be produced that is optimised for <i>E. coli</i> and as such more amenable to synthetic biology experiments.</p> |
| + | <h4><u>RESULTS</u></h4> | ||
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Revision as of 10:19, 21 September 2012
Rationale
Being able to induce gene expression is one of the most important aspect of the biomolecular sciences. It allows us to determine the role of specific genes and to produce them in vast quantities. Chemical induction is commonly used but has some lag time and so is less useful to determine if the role of a developmental gene. As such a fast and reversible method of controlling gene expression needs to be developed, one that does not require a long incubation time. Our project aims to produce a light controlled gene switch by coupling heme oxygenase with a bacteriophytochrome. To achieve this goal we will be using Gibson Assembly.
To truly embrace synthetic biology and modify genes to suit our need we have utilised Gibson Assembly. This allows us to introduce numerous changes to the gene of interest. Our project is a showcase for the power of Gibson Assembly in Synthetic Biology. By starting with an a standard and optimising it for E. coli we will show how simple Gibson Assembly is in altering the gene but maintaining function.
