Team:Macquarie Australia/Project

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                                The Plan
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                            How do you get fuel from waste?  Learn about
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                            Team Alberta's progression from by-product
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                            biomass to a viable biodiesel.
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                            <p><a href="https://2011.igem.org/Team:Alberta/project">
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                            Click here to read more...
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                                The Procedure
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                            New organisms require new
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                            techniques. Learn how Team Alberta
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                            progressed from an idea to a reality
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                            and the steps required to get there.
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                        <a href="https://2011.igem.org/Team:Alberta/Achievements/ProofofConcept">
 
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                                The Product
 
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                            Biodiesel is a viable fuel. See our
 
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                            fuel in action and learn about Team
 
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                            Alberta's plans to make biodiesel
 
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                            production and usage even easier.
 
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                                The Potential
 
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                            Home production and commercial production are
 
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                            viable options for our biodiesel production
 
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                            methods. See Team Alberta's plan to make a
 
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                            small laboratory process into small scale
 
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                            bio-production and large scale.
 
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                            <p><a href="https://2011.igem.org/Team:Alberta/HumanPractices/Bioreactor">
 
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                            Click here to read more...
 
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                WELCOME
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<p>Team Alberta's aim is to aid in the solution of a global
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problem, the fuel crisis, by thinking locally. In Alberta, our main
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industrial practices lay within the oil and gas sector; however, we
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also have a thriving agricultural and forestry-based industry. The
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industrial processes associated with these industries produce
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biomass by-products of little economic value. The aim of our
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project is to convert these by-products into a useful and
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economically viable fuel, biodiesel.</p><br>
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<p>Previous research has been largely
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focused on engineering organisms to metabolize cellulose, a highly
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inefficient approach with very little yield. Here is where our
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approach differs. <b>Why engineer a new organism to perform a function
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organism even better?</b></p><br>
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<p>We have selected the filamentous, ascomycete fungus <i>Neurospora
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    crassa</i>, which is a natural cellulose metabolizer, with the aim
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of creating an organism to efficiently make biodiesel. Our fuel
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will be made by up-regulating fatty acid synthesis and inhibiting
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beta-oxidation, effectively causing the over-production of fatty
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acids within <i>N. crassa</i>. From here we will efficiently esterify
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the fatty acids into fatty acid methyl esters (FAMEs), producing
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a viable fuel.</p>
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<li class="first"><a href="https://2012.igem.org/Team:Macquarie_Australia" accesskey="1" title="">Home</a></li>
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<li><a href="https://igem.org/Team.cgi?year=2012&team_name=Macquarie_Australia" accesskey="2" title="">Official Profile</a></li>
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<li><a href="https://2012.igem.org/Team:Macquarie_Australia/Project" accesskey="4" title="">Project</a></li>
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<li><a href="https://2012.igem.org/Team:Macquarie_Australia/Team" accesskey="3" title="">Meet MQ</a></li>
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<li><a href="https://2012.igem.org/Team:Macquarie_Australia/Safety" accesskey="5" title="">Safety</a></li>
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<li><a href="https://2012.igem.org/Team:Macquarie_Australia/Notebook" accesskey="6" title="">Notebook</a></li>
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<li><a href="https://2012.igem.org/Team:Macquarie_Australia/Attributions" accesskey="7" title="">Sponsors</a></li>
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<li><a href="https://2012.igem.org/Team:Macquarie_Australia/HumanOutreach" accesskey="8" title="">Human Outreach</a></li>
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<li><a href="https://2012.igem.org/Team:Macquarie_Australia/Parts" accesskey="9" title="">Parts</a></li>
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    <a id=ingenuity-img href="https://2011.igem.org/Team:Alberta/project">Ingenuity</a>
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Macquarie_Australia|Home]]
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!align="center"|[[Team:Macquarie_Australia/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Macquarie_Australia Official Team Profile]
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!align="center"|[[Team:Macquarie_Australia/Project|Project]]
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!align="center"|[[Team:Macquarie_Australia/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Macquarie_Australia/Modeling|Modeling]]
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!align="center"|[[Team:Macquarie_Australia/Notebook|Notebook]]
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!align="center"|[[Team:Macquarie_Australia/Safety|Safety]]
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!align="center"|[[Team:Macquarie_Australia/Attributions|Attributions]]
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!align="center"|[[Team:Macquarie_Australia/HumanOutreach|Human Outreach]]
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|}
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== '''Overall project''' ==
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                LEARN
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'''Background:''' Phytochromes are ubiquitous proteins that allow an organism to sense light. These proteins have evolved in unique environments to sense light intensity in different colour ranges. This experiment focuses on constructing a biological switch that uses phytochromes from Deinococcus radiodurans and Agrobacterium tumefaciens. The coupling of heme oxygenase supplies our phytochrome proteins with biliverdin, allowing for the self-assembly of the switch within host systems. The switch is the first stage of a two component light sensor and when expressed at high level, there is a noticeable colour change of the cell when it is activated by light.
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'''Project Aims:''' The objective in this project is to therefore build and characterise a biological light switch in E. coli. This will involve construction of heme-oxygenase and bacteriophytochrome BioBrick parts. This year's research team will be expanding upon the research conducted by last year's iGEM team and the team from 2010. In 2010 the Macquarie Team cloned bacteriophytochrome from two sources. They showed that when one was expressed, it was functionally assembled when incubated with exogenous biliverdin and able to elicit a colour change when excited with far-red light. However, the part created is not directly usable as a BioBrick as it contains an internal EcoRI site (Deinococcus radiodurans phytochrome) and 2 PstI sites (Agrobacterium tumefaciens phytochrome). As biliverdin is not native to E. coli, the addition of heme oxygenase is required for the synthesis of bilivedin, enabling the self-assembly of the light switch. In 2011, the Macquarie Team successfully managed to construct and characterise the heme oxygenase 1 as a BioBrick. They showed, via its green color, that cells expressing the heme oxygenase could degrade heme into biliverdin.
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                ACHIEVE
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            <li>Development of a rapid, systematic method to construct genes for <i>N. crassa</i>
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            <li>Development and utilization of <i> N. crassa </i>as a suitable synthetic biology chassis</li>
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            <li>Creation of 7 parts for use in future synthetic biology projects </li>
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            <li>Design of a self-contained bioreactor apparatus </li>
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<li>Determination of our fuel as an economically viable biodiesel</li>
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In this project we will aim to construct the BioBricks using Gibson cloning as opposed to restriction enzyme digests.
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                INTERACT
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== Project Details==
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=== Part 2 ===
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=== The Experiments ===
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                SPONSORS
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=== Part 3 ===
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== Results ==

Revision as of 00:27, 11 August 2012

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions Human Outreach



Contents

Overall project

Background: Phytochromes are ubiquitous proteins that allow an organism to sense light. These proteins have evolved in unique environments to sense light intensity in different colour ranges. This experiment focuses on constructing a biological switch that uses phytochromes from Deinococcus radiodurans and Agrobacterium tumefaciens. The coupling of heme oxygenase supplies our phytochrome proteins with biliverdin, allowing for the self-assembly of the switch within host systems. The switch is the first stage of a two component light sensor and when expressed at high level, there is a noticeable colour change of the cell when it is activated by light.


Project Aims: The objective in this project is to therefore build and characterise a biological light switch in E. coli. This will involve construction of heme-oxygenase and bacteriophytochrome BioBrick parts. This year's research team will be expanding upon the research conducted by last year's iGEM team and the team from 2010. In 2010 the Macquarie Team cloned bacteriophytochrome from two sources. They showed that when one was expressed, it was functionally assembled when incubated with exogenous biliverdin and able to elicit a colour change when excited with far-red light. However, the part created is not directly usable as a BioBrick as it contains an internal EcoRI site (Deinococcus radiodurans phytochrome) and 2 PstI sites (Agrobacterium tumefaciens phytochrome). As biliverdin is not native to E. coli, the addition of heme oxygenase is required for the synthesis of bilivedin, enabling the self-assembly of the light switch. In 2011, the Macquarie Team successfully managed to construct and characterise the heme oxygenase 1 as a BioBrick. They showed, via its green color, that cells expressing the heme oxygenase could degrade heme into biliverdin.

In this project we will aim to construct the BioBricks using Gibson cloning as opposed to restriction enzyme digests.

Project Details

Part 2

The Experiments

Part 3

Results

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