Team:Macquarie Australia/Notebook

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Contents

Tuesday 7/8/12

(Click on headings to visit methods)

Making liquid media, plates & buffers

  • Liquid LB Media
  • SOC Solution
  • SOB Solution
  • LB Agar Plates

Ampicillin LB Agar Plates: 31 plates

Chloramphenicol LB Agar Plates: 33 Plates

Kanamyacin LB Agar Plates: 32 Plates

  • TB buffer.
  • TAE buffer.
  • EDTA buffer.


Designing Gibson Assembly Fragments

  • Haemoxygenase Gene
  • Deinococcus radiodurans Bacteriophytochrome Gene
  • Agrobacterium tumefaciens Bacteriophytochrome Gene

Tuesday 14/8/12

Pipettes.jpg









(Click on headings to visit methods)

Making Competent Cells

  • Choosing Biobricks
  • Making competent cells
  • Transformations

Outreach Planning Labwork

  • Open Day
  • Secondary Education Seminars

Wednesday 15/8/12

Our open day preparation did not go as planned. Most of our transformations were unsuccessful, we believe this was because SOC was added after the heat shock. As disappointing as this was, there was one little piece of good news.

A single colony of Top 10 E.coli containing GFP insert was identified on a plate of LB agar media.

LB agar and nutrient agar plates were struck using this colony. The colony was also used to inoculate 5mL of nutrient broth. Plates and stock were then incubated at 37°C overnight. Both of the streaked plates and the broth showed significant growth of E.coli colonies which could fluoresce when exposed to UV light.

Unfortunately, Qantas declined to sponsor the effort of getting our team to Hong Kong. This was soon forgotten when the Macquarie Faculty of Medicine, Agilent and the Defence Science Institute (thanks Yagiz) both agreed to sponsor our team. Hong Kong here we come.



Tuesday 21/8/12

Outreach Planning Labwork

  • Secondary education seminars
  • Reattempting glowing bacteria

Making Plates


Tuesday 28/8/12

Outreach Planning

  • High School Presentations: Preparation for 4th September
  • Open Day: Preparation for 8th September at Macquarie University
  • Writing the Abstract for the project due 7th September

Outreach Lab Work

  • Fluorescent Bacteria

Competent Cell Testing


Friday 31/8/12

gBlock fragments

  • gBlock fragments from IDT

Tuesday 04/09/12

Gibson Assembly

  • Gibson Assembly Protocol

Outreach

  • Visit to High School
  • Outreach administration
  • Media preparation for more Fluorescent bacteria

Competent Cell Protocol

  • Generation of Competent Cells

Wednesday 05.09/12


Thursday 06/09/12

We continued on from the Gibson assembly and transformations we performed on the 04/06/12.