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From 2012.igem.org

Contents 1 Tuesday 7/8/12 1.1 Making liquid media, plates & buffers 1.2 Designing Gibson Assembly Fragments 2 Tuesday 14/8/12 2.1 Making Competent Cells 2.2 Outreach Planning Labwork

Tuesday 7/8/12

(Click on headings to visit methods)

Making liquid media, plates & buffers

• Liquid LB Media

• SOC Solution

• SOB Solution

• LB Agar Plates

Ampicillin LB Agar Plates: 31 plates

Chloramphenicol LB Agar Plates: 33 Plates

Kanamyacin LB Agar Plates: 32 Plates

• TB buffer.

• TAE buffer.

• EDTA buffer.

Designing Gibson Assembly Fragments

• Haemoxygenase Gene
• Deinococcus radiodurans Bacteriophytochrome Gene
• Agrobacterium tumefaciens Bacteriophytochrome Gene

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Tuesday 14/8/12

(Click on headings to visit methods)

Making Competent Cells

• Choosing Biobricks
• Making competent cells
• Transformations

Outreach Planning Labwork

• Open Day

• Secondary Education Seminars