Team:MIT/MaterialsAndMethods

From 2012.igem.org

(Difference between revisions)
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<u> Room Temperature Annealing </u>
<u> Room Temperature Annealing </u>
<OL>
<OL>
-
&nbsp<LI>Mix gate and output in a 1.2:1 ratio in a tube.</LI>
+
&nbsp;<LI>Mix gate and output in a 1.2:1 ratio in a tube.</LI>
-
&nbsp<LI>Cover the tube in foil, if you didn't use an opaque/black tube.</LI>
+
&nbsp;<LI>Cover the tube in foil, if you didn't use an opaque/black tube.</LI>
-
&nbsp<LI>Leave the tube on a bench top overnight.</LI>
+
&nbsp;<LI>Leave the tube on a bench top overnight.</LI>
</OL>
</OL>

Revision as of 22:49, 9 September 2012

iGEM 2012

Bacterial

  • Placeholder1
  • Placeholder2

In Vitro

  • Gate Anneals
  • Plate Reader Studies

Mammalian

  • DNA Transfection
  • RNA Transfection

Gate Anneals

Room Temperature Annealing
     
  1. Mix gate and output in a 1.2:1 ratio in a tube.
  2.  
  3. Cover the tube in foil, if you didn't use an opaque/black tube.
  4.  
  5. Leave the tube on a bench top overnight.

Plate Reader Studies

PLACEHOLDER TEXT

RNA Transfection

PLACEHOLDER TEXT

RNA Transfection

PLACEHOLDER TEXT