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Team:Kyoto/Secretion/Notebook
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Revision as of 20:51, 24 September 2012
Contents |
Secretion Notebook
September 18
Gel extraction
写真お願いします。
- LacP-torA-GFP-DT(EcoR1, Xba1) 36.9μg/mL 1.37(260/280) 0.82(260/230)
Ligation
| J23107-tatABCD-pspA-DT(EcoR1, Spe1) 14.2μg/mL | LacP-torA-GFP-DT(EcoR1, Xba1) 36.9μg/mL | Ligation High Ver.2 | total |
|---|---|---|---|
| 8 | 1.5 | 9.5 | 19 |
| LacP-torA-GFP-DT(EcoR1, Xba1) 36.9μg/mL | Ligation High Ver.2 | total |
|---|---|---|
| 1.5 | 1.5 | 3 |
at 4℃ for 1 hour
Transformation
- J23107-tatABCD-pspA-DT-LacP-torA-GFP-DT
- LacP-torA-GFP-DT(EcoR1, Xba1)
Observation through a confocal microscope
We made sample for observation through a confocal microscope We incubated sample with 0mM/0.5mM IPTG for 1.5 hours. Then, we incubated onto medium without IPTG for 2.5 hours.
| IPTG(mM) | medium |
|---|---|
| 0 | LB |
| 0.1 | LB |
| 0.1 | LB with 0.1mM IPTG |
| 0 | LB with 2 percent glucose |
| 0.1 | LB with 2 percent glucose |
| 0 | LB with 5 percent glucose |
| 0.1 | LB with 5 percent glucose |
| 0 | LB with 2 percent glucose |
| 0 | LB ここ途中 |
| 0 | LB |
| 0 | LB |
Then, we incubated onto medium without IPTG for 2.5 hours.
Restriction enzyme processing
| J23101-Kil-DT | BufferM | XbaI | Pst | BSA | MilliQ | total |
|---|---|---|---|---|---|---|
| 15 | 3 | 1 | 1 | 3 | 7 | 30 |
| J23101-Kil-DT | BufferM | XbaI | BSA | MilliQ | total |
|---|---|---|---|---|---|
| 3 | 1 | 0.5 | 1 | 4.5 | 10 |
| J23101-Kil-DT | BufferH | Pst | MilliQ | total |
|---|---|---|---|---|
| 3 | 1 | 0.5 | 5.5 | 10 |
37℃
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