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Team:KIT-Kyoto/c6h12o6
From 2012.igem.org
1 August
| 1-1 | sample1 |
| 2-1 | 6μL |
| 3-1 | 10μL |
| 4-1 | 10μL |
| 5-1 | 3.2μL |
| 6-1 | 3μL |
| 7-1 | 3μL |
| 8-1 | 2μL |
| 9-1 | 62.8μL |
| 10-1 | 100μL |
| temperature | time | cycle |
| 95°C | 2min | |
| 95°C | 15sec | 25cycle |
| 60°C | 30sec | 25cycle |
| 68°C | 2min30sec | 25cycle |
| 68°C | 2min30sec | |
| 14°C | ∞ |
| 1-1 | sample1 | sample2 | sample3 | sample4 |
| 2-1 | 2μL | 2μL | 1μL | 1μL |
| 3-1 | 2μL | 2μL | 2μL | 2μL |
| 4-1 | 2μL | 2μL | 2μL | 2μL |
| 5-1 | 0.8μL | 0.8μL | 0.8μL | 0.8μL |
| 6-1 | 0.6μL | 0.6μL | 0.6μL | 0.6μL |
| 7-1 | 0.6μL | 0.6μL | 0.6μL | 0.6μL |
| 8-1 | 0.4μL | 0.4μL | 0.4μL | 0.4μL |
| 9-1 | 11.6μL | 11.6μL | 12.6μL | 12.6μL |
| 10-1 | 20μL | 20μL | 20μL | 20μL |
| temperature | time | cycle |
| 95°C | 2min | |
| 95°C | 15sec | 25cycle |
| 50°C(sample1,3)or55°C(sample2,4) | 30sec | 25cycle |
| 68°C | 3min30sec | 25cycle |
| 68°C | 3min30sec | |
| 14°C | ∞ |
2 August
| 1-1 | 1-2 | 1-3 |
| 2-1 | 10μL | 20μL |
| 3-1 | 2μL | 4μL |
| 4-1 | 12μL | 25μL |
| 1-2 | |
| DNA sample | 90μL |
| 6×Dye | 18μL |
| 4-1 | 108μL |
| 1-1 | 5μL |
| 2-1 | 10μL |
| 3-1 | 10μL |
| 4-1 | 4μL |
| 5-1 | 3μL |
| 6-1 | 3μL |
| 7-1 | 2μL |
| 8-1 | 63μL |
| 9-1 | 100μL |
| temperature | time | cycle |
| 95°C | 2min | |
| 95°C | 15sec | 35cycle |
| 55°C | 30sec | 35cycle |
| 68°C | 3min30sec | 35cycle |
| 68°C | 3min30sec | |
| 14°C | ∞ |
Result
3 August
| 1-1 | 1μL |
| 2-1 | 0.35μL |
| 3-1 | 7.65 |
| 4-1 | 9μL |
| 1-1 | sample1 | sample2 |
| 2-1 | 2.5μL | 2.5μL |
| 3-1 | 5μL | 5μL |
| 4-1 | 5μL | 5μL |
| 5-1 | 2μL | 2.4μL |
| 6-1 | 1.5μL | 1.5μL |
| 7-1 | 1.5μL | 1.5μL |
| 8-1 | 1μL | 1μL |
| 9-1 | 31.5μL | 31.1μL |
| 10-1 | 50μL | 50μL |
| temperature | time | cycle |
| 95°C | 2min | |
| 95°C | 15sec | 35cycle |
| 55°C | 30sec | 35cycle |
| 68°C | 3min30sec | 35cycle |
| 68°C | 3min30sec | |
| 14°C | ∞ |
4 August
5 August
6 August
7 August
| pDONR | BP TNFA-1 | BP TNFA-2 | |
| DNA sample | 1μL | 1μL | 1μL |
| 3-1 | 1μL | 1μL | 1μL |
| 4-1 | 4μL | 4μL | 4μL |
| 5-1 | 6μL | 6μL | 6μL |
| 1-1 | 2μL |
| 2-1 | 0.5μL |
| 3-1 | 6.5μL |
| 4-1 | 9μL |
8 August
9 Augus
| 1-1 | 1μL |
| 2-1 | 0.5μL |
| 3-1 | 0.2μL |
| 4-1 | 3.3μL |
| 5-1 | 5μL |
| 1-1 | 1-2 |
| 2-1 | 1μL |
| 3-1 | 2μL |
| 4-1 | 2μL |
| 5-1 | 0.8μL |
| 6-1 | 0.6μL |
| 7-1 | 0.6μL |
| 8-1 | 0.4μL |
| 9-1 | 12.6μL |
| 10-1 | 20μL |
| temperature | time | cycle |
| 95°C | 2min | |
| 95°C | 15sec | 25cycle |
| 55°C | 30sec | 25cycle |
| 68°C | 2min30sec | 25cycle |
| 68°C | 2min30sec | |
| 14°C | ∞ |
10 August
11 August
12 August
13 August
| 1-1 | 1μL |
| 2-1 | 0.5μL |
| 3-1 | 0.2μL |
| 4-1 | 3.3μL |
| 5-1 | 5μL |
14 August
| 1-1 | sample1 |
| 2-1 | 6μL |
| 3-1 | 10μL |
| 4-1 | 10μL |
| 5-1 | 3.2μL |
| 6-1 | 3μL |
| 7-1 | 3μL |
| 8-1 | 2μL |
| 9-1 | 62.8μL |
| 10-1 | 100μL |
| temperature | time | cycle |
| 95°C | 2min | |
| 95°C | 15sec | 25cycle |
| 60°C | 30sec | 25cycle |
| 68°C | 2min30sec | 25cycle |
| 68°C | 2min30sec | |
| 14°C | ∞ |
15 August
16 August
17 August
18 August
19 August
20 August
| 1-1 | 1-2 |
| 2-1 | 2-2 |
| 3-1 | 3-2 |
| 4-1 | 4-2 |
21 August
| 1-1 | 1-2 |
| 2-1 | 2-2 |
| 3-1 | 3-2 |
| 4-1 | 4-2 |
