Team:KIT-Kyoto/c6h12o6

From 2012.igem.org

(Difference between revisions)
Line 250: Line 250:
<Table Border Cellspacing="0">
<Table Border Cellspacing="0">
-
<Tr><Td></Td><Td></Td>pDONR<Td>BP TNFA-1</Td><Td>BP TNFA-2</Td></Tr>
+
<Tr><Td></Td><Td>pDONR</Td><Td>BP TNFA-1</Td><Td>BP TNFA-2</Td></Tr>
<Tr><Td>DNA sample</Td><Td>1μL</Td><Td>1μL</Td><Td>1μL</Td></Tr>
<Tr><Td>DNA sample</Td><Td>1μL</Td><Td>1μL</Td><Td>1μL</Td></Tr>
<Tr><Td>3-1</Td><Td>1μL</Td><Td>1μL</Td><Td>1μL</Td></Tr>
<Tr><Td>3-1</Td><Td>1μL</Td><Td>1μL</Td><Td>1μL</Td></Tr>

Revision as of 00:39, 9 September 2012

1 August

1-1sample1
2-16μL
3-110μL
4-110μL
5-13.2μL
6-13μL
7-13μL
8-12μL
9-162.8μL
10-1100μL

temperaturetimecycle
95°C2min
95°C15sec25cycle
60°C30sec25cycle
68°C2min30sec25cycle
68°C2min30sec
14°C

1-1sample1sample2sample3sample4
2-12μL2μL1μL1μL
3-12μL2μL2μL2μL
4-12μL2μL2μL2μL
5-10.8μL0.8μL0.8μL0.8μL
6-10.6μL0.6μL0.6μL0.6μL
7-10.6μL0.6μL0.6μL0.6μL
8-10.4μL0.4μL0.4μL0.4μL
9-111.6μL11.6μL12.6μL12.6μL
10-120μL20μL20μL20μL

temperaturetimecycle
95°C2min
95°C15sec25cycle
50°C(sample1,3)or55°C(sample2,4)30sec25cycle
68°C3min30sec25cycle
68°C3min30sec
14°C

2 August

1-11-21-3
2-110μL20μL
3-12μL4μL
4-112μL25μL

1-2
DNA sample90μL
6×Dye18μL
4-1108μL

1-15μL
2-110μL
3-110μL
4-14μL
5-13μL
6-13μL
7-12μL
8-163μL
9-1100μL

temperaturetimecycle
95°C2min
95°C15sec35cycle
55°C30sec35cycle
68°C3min30sec35cycle
68°C3min30sec
14°C

Result

3 August

1-11μL
2-10.35μL
3-17.65
4-19μL

1-1sample1sample2
2-12.5μL2.5μL
3-15μL5μL
4-15μL5μL
5-12μL2.4μL
6-11.5μL1.5μL
7-11.5μL1.5μL
8-11μL1μL
9-131.5μL31.1μL
10-150μL50μL

temperaturetimecycle
95°C2min
95°C15sec35cycle
55°C30sec35cycle
68°C3min30sec35cycle
68°C3min30sec
14°C

4 August

5 August

6 August

7 August

pDONRBP TNFA-1BP TNFA-2
DNA sample1μL1μL1μL
3-11μL1μL1μL
4-14μL4μL4μL
5-16μL6μL6μL

1-12μL
2-10.5μL
3-16.5μL
4-19μL

8 August

9 Augus

1-11μL
2-10.5μL
3-10.2μL
4-13.3μL
5-15μL

1-11-2
2-11μL
3-12μL
4-12μL
5-10.8μL
6-10.6μL
7-10.6μL
8-10.4μL
9-112.6μL
10-120μL

temperaturetimecycle
95°C2min
95°C15sec25cycle
55°C30sec25cycle
68°C2min30sec25cycle
68°C2min30sec
14°C

10 August

11 August

12 August

13 August

1-11μL
2-10.5μL
3-10.2μL
4-13.3μL
5-15μL

14 August

1-1sample1
2-16μL
3-110μL
4-110μL
5-13.2μL
6-13μL
7-13μL
8-12μL
9-162.8μL
10-1100μL

temperaturetimecycle
95°C2min
95°C15sec25cycle
60°C30sec25cycle
68°C2min30sec25cycle
68°C2min30sec
14°C

15 August

16 August

17 August

18 August

19 August

20 August

1-11-2
2-190μL
3-118μL
4-1108μL

21 August

1-12μL
2-11μL
3-15μL
4-18μL

22 August