Team:KIT-Kyoto/Notebook-week1p
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+ | <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week1p"><img src="https://static.igem.org/mediawiki/2012/1/1b/Side_week1kit.jpg" width="150" height="30"></a></li> | ||
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+ | <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week5p"><img src="https://static.igem.org/mediawiki/2012/e/e7/Side_week5kit.jpg" width="150" height="30"></a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week6p"><img src="https://static.igem.org/mediawiki/2012/e/e8/Side_week6kit.jpg" width="150" height="30"></a></li> | ||
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+ | <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week6"><img src="https://static.igem.org/mediawiki/2012/e/e8/Side_week6kit.jpg" width="150" height="30"></a></li> | ||
+ | <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-week7"><img src="https://static.igem.org/mediawiki/2012/b/b2/Side_weekkit7.jpg" width="150" height="30"></a></li> | ||
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+ | <li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-Meeting-august"><img src="https://static.igem.org/mediawiki/2012/f/fa/Side_augkit.jpg" width="150" height="30"></a></li><li><a href="https://2012.igem.org/Team:KIT-Kyoto/Notebook-Meeting-september"><img src="https://static.igem.org/mediawiki/2012/8/8b/Side_sepkit.jpg" width="150" height="30"></a></li> | ||
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+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <br> | ||
+ | </td> | ||
+ | |||
+ | <td width="800px" height="510px" valign="top"> | ||
+ | <div id="MIGI"> | ||
+ | <h2>August 25th</h2> | ||
+ | <br> | ||
+ | |||
+ | <Table Border Cellspacing="0"> | ||
+ | <Tr><Td> All DNA </Td><Td> 500ng </Td><Td> 1ug </Td></Tr> | ||
+ | <tr><td> pEGFP-C </td><td> 3uL </td><Td> 6uL </Td></tr> | ||
+ | <Tr><Td> H buffer(TOYOBO) </Td><Td> 5uL </Td><Td> 5uL </Td></Tr> | ||
+ | <tr><td> BamHⅠ(TOYOBO) </td><td> 1uL </td><Td> 1uL </Td></tr><tr> | ||
+ | <td> BglⅡ(TOYOBO) </td><td> 1uL </td><Td> 1uL </Td></tr><tr> | ||
+ | <td> 100×BSA </td><td> 0.5uL </td><Td> 0.5uL </Td></tr><tr> | ||
+ | <td> dH<sub>2</sub>O </td><td> 39.5uL </td><Td> 36.5uL </Td></tr><tr> | ||
+ | <td> Total </td><td> 50uL </td><Td> 50uL </Td></tr> | ||
+ | </Table> | ||
+ | <br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/d/d0/0825kit.png" width="500" height="300"> | ||
+ | <br><BR> | ||
+ | <Table Border Cellspacing="0"> | ||
+ | <tr><td> DNA sample </td><td> 23uL </td></tr> | ||
+ | <Tr><Td> 4 buffer(NEB) </Td><Td> 5uL </Td></Tr> | ||
+ | <tr><td> EcoRⅠ-HF(NEB) </td><td> 1uL </td></tr><tr> | ||
+ | <td> SpeⅠ(NEB) </td><td> 1.5uL </td></tr> | ||
+ | <td> 100×BSA </td><td> 0.5uL </td></tr> | ||
+ | <td> dH<sub>2</sub>O </td><td> 19uL </td></tr> | ||
+ | <td> Total <td> 50uL </td></tr> | ||
+ | </Table> | ||
+ | <br><BR> | ||
+ | <Table Border Cellspacing="0"> | ||
+ | <Tr><Td></Td><Td> All samples </Td></Tr> | ||
+ | <tr><td> DNA template </td><td> 0.5uL </td></tr> | ||
+ | <Tr><Td> 10× KOD plus buffer </Td><Td> 10uL </Td></Tr> | ||
+ | <tr><td> 2mM dNTPs </td><td> 10uL </td></tr><tr> | ||
+ | <td> 25mM MgSO4 </td><td> 3.2uL </td></tr> | ||
+ | <td> 10P 5’ primer </td><td> 3uL </td></tr> | ||
+ | <td> 10P 3’ primer </td><td> 3uL </td></tr> | ||
+ | <td> KOD plus </td><td> 2uL </td></tr> | ||
+ | <td> dH<sub>2</sub>O </td><td> 68.3uL </td></tr> | ||
+ | <td> Total <td> 100uL </td></tr> | ||
+ | </Table> | ||
+ | <br><BR> | ||
+ | <Table Border Cellspacing="0"> | ||
+ | <Tr><Td> Temperature </Td><Td> Time </Td><Td> Circle </Td></Tr> | ||
+ | <tr><td> 95℃</td><td> 2min</td><Td></Td></tr> | ||
+ | <Tr><Td> 95℃</Td><Td> 15sec</Td><Td> 25 cycle </Td></Tr> | ||
+ | <tr><td> 58℃</td><td> 30min(UAS) or 2min10sec(pSB1C3)</td><Td> 25 cycle </Td></tr><tr> | ||
+ | <td> 68℃</td><td> 2min30sec</td><Td> 25 cycle </Td></tr> | ||
+ | <td> 68℃</td><td> 2min30sec</td><Td></Td></tr> | ||
+ | <td> 14℃</td><td> ∞</td><Td></Td></tr> | ||
+ | </Table> | ||
+ | <br><br> | ||
+ | |||
+ | <h2>August 26th</h2> | ||
+ | <br> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/6/6d/0826.png" width="500" height="300"> | ||
+ | <br> | ||
+ | <br> | ||
+ | <h2>August 27th</h2> | ||
+ | <br> | ||
+ | <Table Border Cellspacing="0"> | ||
+ | <Tr><Td> pEGFP-C2(cut) </Td><Td> 1uL </Td></Tr> | ||
+ | <tr><td> Ligation high </td><td> 2.5uL </td></tr><tr> | ||
+ | <td> dH<sub>2</sub>O </td><td> 5uL </td></tr><tr> | ||
+ | <td> Total <td> 7.5uL </td></tr> | ||
+ | </Table> | ||
+ | <br><BR> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/e/e6/0826bkit.png" width="500" height="300"> | ||
+ | <br><br> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/9/99/0826ckit.png" width="500" height="300"> | ||
+ | <br><br> | ||
+ | <h2>August 28th</h2> | ||
+ | <br> | ||
+ | |||
+ | <h2>August 29th</h2> | ||
+ | <br> | ||
+ | |||
+ | <Table Border Cellspacing="0"> | ||
+ | <Tr><Td> pEGFP-C2-1, -2, -3, -4 </Td><Td> 1uL </Td></Tr> | ||
+ | <tr><td> 10×H buffer(TOYOBO) </td><td> 0.5uL </td></tr><tr> | ||
+ | <tr><td> XhoⅠ </td><td> 0.1uL </td></tr><tr> | ||
+ | <tr><td> PstⅠ </td><td> 0.1uL </td></tr><tr> | ||
+ | <td> dH<sub>2</sub>O </td><td> 3.3uL </td></tr><tr> | ||
+ | <td> Total <td> 5uL </td></tr> | ||
+ | </Table> | ||
+ | <br><BR> | ||
+ | |||
+ | <img src="https://static.igem.org/mediawiki/2012/9/97/0828kit.png" width="500" height="300"> | ||
+ | <br><br> | ||
+ | <Table Border Cellspacing="0"> | ||
+ | <Tr><Td></Td><Td> All samples </Td></Tr> | ||
+ | <Tr><Td> DNA template </Td><Td> 0.5uL </Td></Tr> | ||
+ | <tr><td> 10× KOD plus buffer </td><td> 10uL </td></tr> | ||
+ | <Tr><Td> 2mM dNTPs </Td><Td> 10uL </Td></Tr> | ||
+ | <Tr><Td> 25mM MgSO4 </Td><Td> 3.2uL </Td></Tr> | ||
+ | <td> 10P 5’ primer </td><td> 3uL </td></tr> | ||
+ | <Tr><td> 10P 3’ primer </td><td> 3uL </td></tr> | ||
+ | <Tr><td> KOD plus </td><td> 2uL </td></tr> | ||
+ | <Tr><td> dH<sub>2</sub>O </td><td> 68.3uL </td></tr> | ||
+ | <Tr><td> Total </td><td> 100uL </td></tr> | ||
+ | </Table> | ||
+ | <br><BR> | ||
+ | |||
+ | <Table Border Cellspacing="0"> | ||
+ | <Tr><Td> Temperature </Td><Td> Time </Td><Td> Cycle </Td></Tr> | ||
+ | <tr><td> 95℃ </td><td> 2min </td><Td></Td></tr> | ||
+ | <Tr><Td> 95℃ </Td><Td> 15sec </Td><Td> 25 cycle </Td></Tr> | ||
+ | <tr><td> 58℃ </td><td> 30sec </td><Td> 25 cycle </Td></tr><tr> | ||
+ | <td> 68℃ </td><td> 30sec(UAS) or 1min(EGFP) </td><Td> 25 cycle </Td></tr> | ||
+ | <tr><td> 68℃ </td><td> 30sec(UAS) or 1min(EGFP) </td><Td></Td></tr> | ||
+ | <tr><td> 14℃ </td><td> ∞ </td><Td></Td></tr> | ||
+ | </Table> | ||
+ | <br><BR> | ||
+ | <img src="https://static.igem.org/mediawiki/2012/d/d2/0829b.png" width="500" height="300"> | ||
+ | <br><br> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </table> | ||
+ | </td></tr> | ||
+ | |||
+ | </table> | ||
+ | </body> | ||
+ | |||
+ | |||
+ | |||
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Revision as of 09:57, 25 September 2012
August 25th
August 26thAugust 27th
August 28thAugust 29th
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