Team:KAIST Korea/Notebook Labnote/2012 10 temp

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KAIST Korea 2012 iGEM

Notebook : Labnote-October

Labnote

October

October 1st 2012

No Special Event!
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October 2nd 2012

No Special Event!
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October 3rd 2012

No Special Event!
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October 4th 2012

No Special Event!
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October 5th 2012

No Special Event!
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October 6th 2012

No Special Event!
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October 7th 2012

No Special Event!
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October 8th 2012

No Special Event!
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October 9th 2012

No Special Event!
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October 10th 2012


 Flip Flop

LuxI and Xis OE template preparation
Results

2_1010Fig1



pAutoIntegrase cloning check and Xis template vector prep
Results

2_1010Fig2

pAutoIntegrase 1-10 – HindIII cut
pGEM-Xis 1-2 – EcoRI cut



insert DNA preparation with OE PCR
Results

2_1010Fig3

┗ pAutoSimple(1-3) and pAutoSimpleInverted(4-6)

2_1010Fig4

┗ pAutoSimple and pAutoSimpleInverted (1 and 2, respectively)/ pAuto(1-3) and pAutoInverted(4-6)
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October 11th 2012


 Flip Flop

Additional templates
Results

2_1011Fig1

┗ Integrase and Excisionase template

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October 12th 2012

No Special Event!
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October 13th 2012

No Special Event!
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October 14th 2012


 Flip Flop

pAutoIntegrase Cloning
Insert DNA – OE PCR

Results

2_1014Fig1

Cloning into pSB4A5 is performed with gel extracted DNA fragment.

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October 15th 2012

No Special Event!
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October 16th 2012


 Flip Flop

pAutoSimple inserts nested PCR and aiiA template
Results

2_1016Fig1



pAuto, pAutoInverted, pAutoSimple, pAutoSimpleInverted miniprep and RE cut
Results

2_1016Fig2

single digestion with HindIII.
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October 17th 2012


 Flip Flop

pAuto OE PCR and Xis, simple bFMO, and bFMO template preparation
Results

2_1017Fig1

sizes ok


pAuto insert DNA preparation
Results

2_1017Fig2

intermediate fragment nested PCR

2_1017Fig3

Full pAuto insert DNA construct OE PCR

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October 18th 2012


 Flip Flop

pAutoIntegrase enz cut check with NotI
Results

2_1018Fig1

Lane 3, 6, 8 seem to be cloned.
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October 19th 2012


 Flip Flop

pAutoIntegrase Alternative sequencing with fragments amplification
Results

2_1019Fig1

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October 20th 2012

No Special Event!
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October 21st 2012

No Special Event!
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October 22nd 2012

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October 23rd 2012

No Special Event!
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October 24th 2012


 Flip Flop

OE template preparation
Results

2_1024Fig1

gp47, aiiA, LuxI, bFMO for simple and pAuto

2_1024Fig1

promoter and Inverted one

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October 25th 2012

 Flip Flop

pAuto intermediate fragment OE PCR
Results

1025Fig1


OE PCR with new primer
Results

2_1025Fig2

pAutoSimple/inverted

2_1025Fig2

pAuto/inverted

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October 26th 2012

No Special Event!
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October 27th 2012

No Special Event!
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October 28th 2012

No Special Event!
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October 29th 2012

No Special Event!
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October 30th 2012

No Special Event!
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October 31st 2012

No Special Event!
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