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| - | {{:Team:KAIST_Korea/top_home}}
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| - | {{:Team:KAIST_Korea/navi}}
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| - | <html>
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| - | <head>
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| - | <link href='http://fonts.googleapis.com/css?family=Gloria+Hallelujah|Open+Sans:600italic,400,800' rel='stylesheet' type='text/css'>
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| - | <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.4.2/jquery.min.js"> </script>
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| - | <script type="text/javascript">
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| - | WebFontConfig = {
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| - | google: { families: [ 'Open+Sans:600italic,400,800:latin' ]|[ 'Gloria+Hallelujah::latin' ]}
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| - | };
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| - | (function() {
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| - | var wf = document.createElement('script');
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| - | wf.src = ('https:' == document.location.protocol ? 'https' : 'http') +
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| - | '://ajax.googleapis.com/ajax/libs/webfont/1/webfont.js';
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| - | wf.type = 'text/javascript';
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| - | wf.async = 'true';
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| - | var s = document.getElementsByTagName('script')[0];
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| - | s.parentNode.insertBefore(wf, s);
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| - | })();
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| - | </script>
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| - | <style>
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| - | @import url(http://fonts.googleapis.com/css?family=Gloria+Hallelujah|Open+Sans:600italic,400,800);
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| - | #kaistcontent
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| - | {
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| - | position: relative;
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| - | background-color:transparent;
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| - | width:690px;
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| - | font-family: 'Open Sans', sans-serif;
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| - | font-size:10pt;
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| - | line-height:120%;
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| - | text-align:justify;
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| - | padding: 0px 15px 0px 15px;
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| - | left:300px;
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| - | top:-70px;
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| - | z-index: 2;
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| - | }
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| | | | |
| - | #kaistcontent #starter-grad
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| - | {
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| - | width:15px;
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| - | height:100px;
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| - | float:left;
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| - | margin:0px 15px 2px 0px;
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| - | }
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| - |
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| - | #kaistcontent #starter
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| - | {
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| - | font-family: 'Open Sans', sans-serif;
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| - | font-size:14pt;
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| - | color: #08185d;
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| - | padding:0px 0px 0px 10px;
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| - | }
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| - |
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| - | #kaistcontent #sub-title
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| - | {
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| - | font-family: 'Open Sans', sans-serif;
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| - | color:#848383;
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| - | font-size: 15pt;
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| - | line-height:100%;
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| - | font-weight:bold;
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| - | font-style:italic;
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| - | }
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| - |
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| - | #kaistcontent h1
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| - | {
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| - | font-family: 'Open Sans', sans-serif;
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| - | color:#5c5858;
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| - | font-weight:bold;
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| - | font-size:24pt;
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| - | line-height:110%;
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| - | }
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| - |
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| - | #kaistcontent h3
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| - | {
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| - | font-family: 'Open Sans', sans-serif;
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| - | color:#181818;
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| - | font-size:14pt;
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| - | line-height:110%;
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| - | }
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| - |
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| - | #kaistcontent h4
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| - | {
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| - | font-family: 'Open Sans', sans-serif;
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| - | color:#585858;
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| - | font-size:12pt;
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| - | line-height:110%;
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| - | }
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| - |
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| - | #kaistcontent ul
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| - | {
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| - | list-style-type:disc;
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| - | padding: 0px 0px 0px 30px;
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| - | }
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| - |
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| - | #kaistcontent li
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| - | {
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| - | list-style-type:inherit;
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| - | }
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| - |
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| - | #kaistcontent #figure
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| - | {
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| - | margin-left:auto;
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| - | margin-right:auto;
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| - | }
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| - | #kaistcontent ol
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| - | {
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| - | list-style-type:decimal;
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| - | padding: 0px 0px 0px 30px;
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| - | }
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| - |
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| - | #kaistcontent #sub-sub-title
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| - | {
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| - | font-family: 'Open Sans', sans-serif;
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| - | color:#848383;
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| - | font-weight:bold;
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| - | font-size:14pt;
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| - | line-height:110%;
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| - | }
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| - | #kaistcontent section
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| - | {
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| - | font-family: 'Open Sans', sans-serif;
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| - | font-size:10pt;
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| - | background-color:#f4f4f4;
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| - | border-style:solid;
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| - | border-width:1px;
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| - | border-color:#EEE;
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| - | padding:2px 10px 10px 10px;
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| - | margin:0px 0px 35px 0px;
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| - | background-image:url('https://static.igem.org/mediawiki/2012/a/a0/KAIST_labnote_logo.png');
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| - | background-repeat:no-repeat;
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| - | opacity:0.9;
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| - |
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| - | }
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| - | #kaistcontent #little
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| - | {
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| - | line-height:140%;
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| - | margin : 0px 15px 0px 15px;
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| - | }
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| - |
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| - | #kaistcontent #content-note
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| - | {
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| - | font-family: 'Open Sans', sans-serif;
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| - | line-height:120%;
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| - |
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| - | }
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| - |
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| - | #kaistcontent section .note-title
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| - | {
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| - | font-family: 'Open Sans', sans-serif;
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| - | font-size:12pt;
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| - | font-weight:bold;
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| - | color: #666;
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| - | text-align:center;
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| - | line-height:140%;
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| - | margin:10px 0px 15px 0px;
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| - | }
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| - | #kaistcontent section .date
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| - | {
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| - | font-family: 'Open Sans', sans-serif;
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| - | font-size:12pt;
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| - | color:#f4f4f4;
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| - | }
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| - | </style>
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| - | </head>
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| - |
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| - | <body>
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| - | <div id="main">
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| - | <img id="top-img" src="https://static.igem.org/mediawiki/2012/6/6d/Back_home.PNG"/>
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| - |
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| - | <div id="menucontent">
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| - | <img src="https://static.igem.org/mediawiki/2012/4/4d/Note_ico.png"></img>
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| - | <a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Overview"><img src="https://static.igem.org/mediawiki/2012/e/e3/Overview_ico.png"/></a>
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| - | <a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Protocol"><img src="https://static.igem.org/mediawiki/2012/b/b6/Protocol_ico.png"/></a>
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| - | <a href="https://2012.igem.org/Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e0/Labnote_ico.png"/></a>
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| - | </div>
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| - |
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| - | <div id="top-img-description-box"><span style="font-size:22px;" id="top-img-description">Notebook : Labnote-October</span></div>
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| - | <img id="horizontal-line" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img>
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| - |
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| - | <div id="kaistcontent">
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| - | <div>
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| - | <img id="starter-grad" style="height:80px;" src="https://static.igem.org/mediawiki/2012/9/95/Starter_gradient_kaist.png"></img>
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| - | <h1>Labnote</h1>
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| - | <span id="sub-title">October</span></br></br>
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| - | </div>
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| - |
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| - | <section id="1">
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| - | <div class="date">October 1<sup>st</sup> 2012</div></br>
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
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| - | </br>
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| - |
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| - | </div>
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| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
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| - |
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| - | <section id="2">
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| - | <div class="date">October 2<sup>nd</sup> 2012</div></br>
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| - |
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
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| - | </br>
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| - |
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| - | </div>
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| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
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| - | <section id="3">
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| - | <div class="date">October 3<sup>rd</sup> 2012</div></br>
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
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| - | </br>
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| - |
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| - | </div>
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| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
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| - | <section id="4">
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| - | <div class="date">October 4<sup>th</sup> 2012</div></br>
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
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| - | </br>
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| - |
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| - | </div>
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| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
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| - | <section id="5">
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| - | <div class="date">October 5<sup>th</sup> 2012</div></br>
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
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| - | </br>
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| - |
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| - | </div>
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| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
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| - | <section id="6">
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| - | <div class="date">October 6<sup>th</sup> 2012</div></br>
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
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| - | </br>
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| - |
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| - | </div>
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| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
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| - | <section id="7">
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| - | <div class="date">October 7<sup>th</sup> 2012</div></br>
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
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| - | </br>
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| - |
| |
| - | </div>
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| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
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| - | <section id="8">
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| - | <div class="date">October 8<sup>th</sup> 2012</div></br>
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
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| - | </br>
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| - |
| |
| - | </div>
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| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
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| - | <section id="9">
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| - | <div class="date">October 9<sup>th</sup> 2012</div></br>
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
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| - | </br>
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| - |
| |
| - | </div>
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| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
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| - | <section id="10">
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| - | <div class="date">October 10<sup>th</sup> 2012</div></br>
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| - | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
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| - |
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| - | <div class="note-title">LuxI and Xis OE template preparation</div>
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| - |
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| - | <div id="content_note" >
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| - | <b>Results</b></br></br>
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| - |
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| - | <div align="center"><img id="figure" alt="2_1010Fig1" src="https://static.igem.org/mediawiki/2012/3/39/KAIST_LABNOTE101012_Fig1.png"></img></div>
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| - | <div style="clear:both;"></div>
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| - |
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| - | </br>
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| - | </div>
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| - |
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| - | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br>
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| - |
| |
| - | <div class="note-title">pAutoIntegrase cloning check and Xis template vector prep</div>
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| - |
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| - | <div id="content_note" >
| |
| - |
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| - | <b>Results</b></br></br>
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| - |
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| - | <div align="center"><img id="figure" alt="2_1010Fig2" src="https://static.igem.org/mediawiki/2012/4/4f/KAIST_LABNOTE101012_Fig2.png"></img></div>
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| - | <div style="clear:both;"></div>
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| - |
| |
| - | </br>
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| - |
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| - | <span id="little">pAutoIntegrase 1-10 – HindIII cut</br></span>
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| - | <span id="little">pGEM-Xis 1-2 – EcoRI cut</br></span>
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| - |
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| - |
| |
| - | </br>
| |
| - | </div>
| |
| - |
| |
| - | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br>
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| - |
| |
| - | <div class="note-title">insert DNA preparation with OE PCR</div>
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| - |
| |
| - | <div id="content_note" >
| |
| - |
| |
| - | <b>Results</b></br></br>
| |
| - |
| |
| - | <div align="center"><img id="figure" alt="2_1010Fig3" src="https://static.igem.org/mediawiki/2012/d/df/KAIST_LABNOTE101012_Fig3.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - |
| |
| - | <span id="little">┗ pAutoSimple(1-3) and pAutoSimpleInverted(4-6)</span></br>
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| - |
| |
| - | </br>
| |
| - | <div align="center"><img id="figure" alt="2_1010Fig4" src="https://static.igem.org/mediawiki/2012/7/7d/KAIST_LABNOTE101012_Fig4.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - |
| |
| - | <span id="little">┗ pAutoSimple and pAutoSimpleInverted (1 and 2, respectively)/ pAuto(1-3) and pAutoInverted(4-6)</span>
| |
| - |
| |
| - | </br>
| |
| - | </div>
| |
| - |
| |
| - |
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - |
| |
| - | </section>
| |
| - | <section id="11">
| |
| - | <div class="date">October 11<sup>th</sup> 2012</div></br>
| |
| - | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
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| - |
| |
| - | <div class="note-title">Additional templates</div>
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| - |
| |
| - | <div id="content_note" >
| |
| - |
| |
| - | <b>Results</b></br></br>
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| - |
| |
| - | <div align="center"><img id="figure" alt="2_1011Fig1" src="https://static.igem.org/mediawiki/2012/2/2d/KAIST_LABNOTE101112_Fig1.png"></img></div>
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| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - | <div align='center'>
| |
| - | <span id="little">┗ Integrase and Excisionase template</span></div>
| |
| - |
| |
| - | </br>
| |
| - |
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - | </section>
| |
| - | <section id="12">
| |
| - | <div class="date">October 12<sup>th</sup> 2012</div></br>
| |
| - | <div id="content_note" >
| |
| - | <span id="little">No Special Event!</span>
| |
| - | </br>
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - |
| |
| - |
| |
| - | </section>
| |
| - | <section id="13">
| |
| - | <div class="date">October 13<sup>th</sup> 2012</div></br>
| |
| - | <div id="content_note" >
| |
| - | <span id="little">No Special Event!</span>
| |
| - | </br>
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - | </section>
| |
| - | <section id="14">
| |
| - | <div class="date">October 14<sup>th</sup> 2012</div></br>
| |
| - | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
| |
| - |
| |
| - | <div class="note-title">pAutoIntegrase Cloning</div>
| |
| - |
| |
| - | <div id="content_note" >
| |
| - |
| |
| - | <span id="little">Insert DNA – OE PCR</span>
| |
| - | </br></br>
| |
| - | <b>Results</b></br></br>
| |
| - |
| |
| - | <div align="center"><img id="figure" alt="2_1014Fig1" src="https://static.igem.org/mediawiki/2012/1/12/KAIST_LABNOTE101412_Fig1.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - | <div align='center'>
| |
| - | <span id="little">Cloning into pSB4A5 is performed with gel extracted DNA fragment.</span></div>
| |
| - |
| |
| - | </br>
| |
| - |
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - | </section>
| |
| - | <section id="15">
| |
| - | <div class="date">October 15<sup>th</sup> 2012</div></br>
| |
| - | <div id="content_note" >
| |
| - | <span id="little">No Special Event!</span>
| |
| - | </br>
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - | </section>
| |
| - | <section id="16">
| |
| - | <div class="date">October 16<sup>th</sup> 2012</div></br>
| |
| - | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
| |
| - |
| |
| - | <div class="note-title">pAutoSimple inserts nested PCR and aiiA template</div>
| |
| - |
| |
| - | <div id="content_note" >
| |
| - |
| |
| - | <b>Results</b></br></br>
| |
| - |
| |
| - | <div align="center"><img id="figure" alt="2_1016Fig1" src="https://static.igem.org/mediawiki/2012/c/cb/KAIST_LABNOTE101612_Fig1.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - | </div>
| |
| - |
| |
| - | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br>
| |
| - |
| |
| - | <div class="note-title">pAuto, pAutoInverted, pAutoSimple, pAutoSimpleInverted miniprep and RE cut</div>
| |
| - |
| |
| - | <div id="content_note" >
| |
| - |
| |
| - | <b>Results</b></br></br>
| |
| - |
| |
| - | <div align="center"><img id="figure" alt="2_1016Fig2" src="https://static.igem.org/mediawiki/2012/c/c4/KAIST_LABNOTE101612_Fig2.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - |
| |
| - | <span id="little">single digestion with HindIII.</span>
| |
| - |
| |
| - | </br>
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - |
| |
| - | </section>
| |
| - | <section id="17">
| |
| - | <div class="date">October 17<sup>th</sup> 2012</div></br>
| |
| - | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
| |
| - |
| |
| - | <div class="note-title">pAuto OE PCR and Xis, simple bFMO, and bFMO template preparation</div>
| |
| - |
| |
| - | <div id="content_note" >
| |
| - |
| |
| - | <b>Results</b></br></br>
| |
| - |
| |
| - | <div align="center"><img id="figure" alt="2_1017Fig1" src="https://static.igem.org/mediawiki/2012/9/9e/KAIST_LABNOTE101712_Fig1.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - |
| |
| - | <span id="little">sizes ok</span>
| |
| - |
| |
| - | </br>
| |
| - |
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br>
| |
| - |
| |
| - | <div class="note-title">pAuto insert DNA preparation</div>
| |
| - |
| |
| - | <div id="content_note" >
| |
| - |
| |
| - | <b>Results</b></br></br>
| |
| - |
| |
| - | <div align="center"><img id="figure" alt="2_1017Fig2" src="https://static.igem.org/mediawiki/2012/5/56/KAIST_LABNOTE101712_Fig2.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - | <div align='center'>
| |
| - | <span id="little">intermediate fragment nested PCR</span></div>
| |
| - |
| |
| - | </br>
| |
| - | <div align="center"><img id="figure" alt="2_1017Fig3" src="https://static.igem.org/mediawiki/2012/7/79/KAIST_LABNOTE101712_Fig3.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - | <div align='center'>
| |
| - | <span id="little">Full pAuto insert DNA construct OE PCR</span></div>
| |
| - |
| |
| - | </br>
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - | </section>
| |
| - | <section id="18">
| |
| - | <div class="date">October 18<sup>th</sup> 2012</div></br>
| |
| - | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
| |
| - |
| |
| - | <div class="note-title">pAutoIntegrase enz cut check with NotI</div>
| |
| - |
| |
| - | <div id="content_note" >
| |
| - |
| |
| - | <b>Results</b></br></br>
| |
| - |
| |
| - | <div align="center"><img id="figure" alt="2_1018Fig1" src="https://static.igem.org/mediawiki/2012/6/66/KAIST_LABNOTE101812_Fig1.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - |
| |
| - | <span id="little">Lane 3, 6, 8 seem to be cloned.</span>
| |
| - |
| |
| - | </br>
| |
| - |
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - |
| |
| - | </section>
| |
| - | <section id="19">
| |
| - | <div class="date">October 19<sup>th</sup> 2012</div></br>
| |
| - | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
| |
| - |
| |
| - | <div class="note-title">pAutoIntegrase Alternative sequencing with fragments amplification</div>
| |
| - |
| |
| - | <div id="content_note" >
| |
| - |
| |
| - | <b>Results</b></br></br>
| |
| - |
| |
| - | <div align="center"><img id="figure" alt="2_1019Fig1" src="https://static.igem.org/mediawiki/2012/9/9d/KAIST_LABNOTE101912_Fig1.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - |
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - |
| |
| - | </section>
| |
| - | <section id="20">
| |
| - | <div class="date">October 20<sup>th</sup> 2012</div></br>
| |
| - | <div id="content_note" >
| |
| - | <span id="little">No Special Event!</span>
| |
| - | </br>
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - |
| |
| - | </section>
| |
| - | <section id="21">
| |
| - | <div class="date">October 21<sup>st</sup> 2012</div></br>
| |
| - | <div id="content_note" >
| |
| - | <span id="little">No Special Event!</span>
| |
| - | </br>
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - | </section>
| |
| - | <section id="22">
| |
| - | <div class="date">October 22<sup>nd</sup> 2012</div></br>
| |
| - | <div id="content_note" >
| |
| - | <span id="little">No Special Event!</span>
| |
| - | </br>
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - | </section>
| |
| - | <section id="23">
| |
| - | <div class="date">October 23<sup>rd</sup> 2012</div></br>
| |
| - | <div id="content_note" >
| |
| - | <span id="little">No Special Event!</span>
| |
| - | </br>
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - | </section>
| |
| - | <section id="24">
| |
| - | <div class="date">October 24<sup>th</sup> 2012</div></br>
| |
| - | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
| |
| - |
| |
| - | <div class="note-title">OE template preparation</div>
| |
| - |
| |
| - | <div id="content_note" >
| |
| - |
| |
| - |
| |
| - | <b>Results</b></br></br>
| |
| - |
| |
| - | <div align="center"><img id="figure" alt="2_1024Fig1" src="https://static.igem.org/mediawiki/2012/9/96/KAIST_LABNOTE102412_Fig1.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - | <div align = 'center'>
| |
| - | <span id="little">gp47, aiiA, LuxI, bFMO for simple and pAuto</span></div>
| |
| - |
| |
| - | </br>
| |
| - | <div align="center"><img id="figure" alt="2_1024Fig1" src="https://static.igem.org/mediawiki/2012/5/52/KAIST_LABNOTE102412_Fig2.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - | <div align = 'center'>
| |
| - | <span id="little">promoter and Inverted one</span></div>
| |
| - |
| |
| - | </br>
| |
| - |
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - | </section>
| |
| - | <section id="25">
| |
| - | <div class="date">October 25<sup>th</sup> 2012</div></br>
| |
| - | <div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
| |
| - | <div class="note-title">pAuto intermediate fragment OE PCR</div>
| |
| - |
| |
| - | <div id="content_note" >
| |
| - |
| |
| - | <b>Results</b></br></br>
| |
| - |
| |
| - | <div align="center"><img id="figure" alt="1025Fig1" src="https://static.igem.org/mediawiki/2012/f/f1/KAIST_LABNOTE102512_Fig1.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - |
| |
| - | </div>
| |
| - | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br>
| |
| - |
| |
| - | <div class="note-title">OE PCR with new primer</div>
| |
| - |
| |
| - | <div id="content_note" >
| |
| - |
| |
| - |
| |
| - | <b>Results</b></br></br>
| |
| - |
| |
| - | <div align="center"><img id="figure" alt="2_1025Fig2" src="https://static.igem.org/mediawiki/2012/a/a9/KAIST_LABNOTE102512_Fig2.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - | <div align='center'>
| |
| - | <span id="little">pAutoSimple/inverted</span></div>
| |
| - |
| |
| - | </br>
| |
| - | <div align="center"><img id="figure" alt="2_1025Fig2" src="https://static.igem.org/mediawiki/2012/9/9d/KAIST_LABNOTE102512_Fig3.png"></img></div>
| |
| - | <div style="clear:both;"></div>
| |
| - |
| |
| - | </br>
| |
| - | <div align='center'>
| |
| - | <span id="little">pAuto/inverted</span></div>
| |
| - |
| |
| - | </br>
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - |
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| - | </section>
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| - | <section id="26">
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| - | <div class="date">October 26<sup>th</sup> 2012</div></br>
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
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| - | </br>
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| - |
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| - | </div>
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| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
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| - | <section id="27">
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| - | <div class="date">October 27<sup>th</sup> 2012</div></br>
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
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| - | </br>
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| - |
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| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
| |
| - | <section id="28">
| |
| - | <div class="date">October 28<sup>th</sup> 2012</div></br>
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
| |
| - | </br>
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| - |
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| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
| |
| - | <section id="29">
| |
| - | <div class="date">October 29<sup>th</sup> 2012</div></br>
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| - | <div id="content_note" >
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| - | <span id="little">No Special Event!</span>
| |
| - | </br>
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
| |
| - | </section>
| |
| - | <section id="30">
| |
| - | <div class="date">October 30<sup>th</sup> 2012</div></br>
| |
| - | <div id="content_note" >
| |
| - | <span id="little">No Special Event!</span>
| |
| - | </br>
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
| |
| - | <section id="31">
| |
| - | <div class="date">October 31<sup>st</sup> 2012</div></br>
| |
| - | <div id="content_note" >
| |
| - | <span id="little">No Special Event!</span>
| |
| - | </br>
| |
| - |
| |
| - | </div>
| |
| - | <a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="https://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="https://static.igem.org/mediawiki/2012/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
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| - | </section>
| |
| - | </br></br>
| |
| - | </div>
| |
| - | </div>
| |
| - |
| |
| - | </body>
| |
| - | </html>
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| - | {{:Team:KAIST_Korea/footer}}
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| - | <!-- sample
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| - | </br><div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:260px;margin:0px 5px 0px 0px; "src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='https://static.igem.org/mediawiki/2012/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:260px; margin:0px 0px 0px 5px;"src="https://static.igem.org/mediawiki/2012/1/1b/KAIST_horizontal_gray.png"/></div></br>
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| - |
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| - | <div class="note-title">TITLE</div>
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| - |
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| - | <div id="content_note" >
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| - |
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| - | <span id="little">CONTENT</span>
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| - | </br></br>
| |
| - | <b>Results</b></br></br>
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| - |
| |
| - | <div align="center"><img id="figure" alt="2_08##Fig#" src="#"></img></div>
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| - | <div style="clear:both;"></div>
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| - | </br>
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| - |
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| - | <span id="little"></span>
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| - | </br>
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| - |
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| - |
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| - | </div>
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| - |
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| - | </br><div align="center"><img style="width:670px; height:2px;" src="https://static.igem.org/mediawiki/2012/7/7f/Horizontal_line_kaist.png"></img></div></br>
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| - |
| |
| - | <div class="note-title">TITLE2</div>
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| - |
| |
| - | <div id="content_note" >
| |
| - |
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| - | <span id="little">CONTENT2</span>
| |
| - | </br></br>
| |
| - | <b>Results</b></br></br>
| |
| - |
| |
| - | <div align="center"><img id="figure" alt="2_08##Fig#" src="#"></img></div>
| |
| - | <div style="clear:both;"></div>
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| - |
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| - | </br>
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| - |
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| - | <span id="little"></span>
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| - |
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| - | </br>
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| - | </div>
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| - | -->
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