Team:KAIST Korea/Notebook Labnote/2012 10

From 2012.igem.org

(Difference between revisions)
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<section id="2">
<section id="2">
<div class="date">October 2<sup>nd</sup> 2012</div></br>
<div class="date">October 2<sup>nd</sup> 2012</div></br>
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<div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="http://2012.igem.org/wiki/images/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='http://2012.igem.org/wiki/images/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:269px; margin:0px 0px 0px 5px;"src="http://2012.igem.org/wiki/images/1/1b/KAIST_horizontal_gray.png"/></div></br>
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<div class="note-title">bFMO template preparation with new primer</div>
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<div id="content_note" >
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<div id="content_note" >
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<span id="little">No Special Event!</span>
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</br>
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<b>Results</b></br></br>
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<div align="center"><img id="figure" alt="0818Fig1" src="http://2012.igem.org/wiki/images/a/a3/121002.PNG"></img></div>
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<div style="clear:both;"></div>
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<span id="little"></br>We have found that our bFMO sequence was wrong. So that we have ordered new primers with right sequence.</br></span>
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</div>
</div>
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<section id="3">
<section id="3">
<div class="date">October 3<sup>rd</sup> 2012</div></br>
<div class="date">October 3<sup>rd</sup> 2012</div></br>
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<div id="content_note" >
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<div id="linegray" align="center" style="font-size:15px; font-weight:bold; color:#888;"><img style="height:3px;width:269px;margin:0px 5px 0px 0px; "src="http://2012.igem.org/wiki/images/1/1b/KAIST_horizontal_gray.png"/><img style="width:40px; height:28px;float:bottom;"id="flipflop" src='http://2012.igem.org/wiki/images/2/22/KAIST_FlipFlop.png'/> Flip Flop<img style="height:3px;width:269px; margin:0px 0px 0px 5px;"src="http://2012.igem.org/wiki/images/1/1b/KAIST_horizontal_gray.png"/></div></br>
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<span id="little">No Special Event!</span>
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<div class="note-title">Visualization of inversion from GFP to RFP</div>
-
</br>
+
 
 +
<div id="content_note" >
 +
<b>Procedure</b></br></br>
 +
 +
<span id="little"></br>bfmo gene is constructed in the vector(pJ401, Km registance) and transformed into DH5α.</br>50λ cell from stock is inoculated in 5mL LB </br></span>
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 +
</br><div align="center"><img style="width:670px; height:2px;" src="http://2012.igem.org/wiki/images/7/7f/Horizontal_line_kaist.png"></img></div></br>
 +
<div class="note-title"> Visualization of inversion from GFP to RFP</div>
 +
 +
<div id="content_note" >
 +
<b>Procedure</b></br></br>
 +
<span id="little">We sampled the cell every 3hr. We induced one group of samples and un-induced another group of samples as a control. We took the picture of cell with confocal microscope at absorbance 500nm for GFP expression and 600nm for RFP expression.  </br>
 +
</span>
 +
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="0818Fig1" src="http://2012.igem.org/wiki/images/a/ae/121003.PNG"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</br></br>
 +
<b>Discussion</b></br></br>
 +
<span id="little">We obtained proper data. Un-induced sample doesn’t show measurable RFP expression throughout the culture. However, induced sample shows the increase of RFP expression. Increase of GFP level in induced sample is due to the stability of GFP and time delay before inversion. </span>
 +
 +
</div>
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 +
</br><div align="center"><img style="width:670px; height:2px;" src="http://2012.igem.org/wiki/images/7/7f/Horizontal_line_kaist.png"></img></div></br>
 +
<div class="note-title"> Insert DNA preparation with OE pcr</div>
 +
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="0818Fig1" src="http://2012.igem.org/wiki/images/2/24/121003-1.PNG"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
</div>
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 +
</br><div align="center"><img style="width:670px; height:2px;" src="http://2012.igem.org/wiki/images/7/7f/Horizontal_line_kaist.png"></img></div></br>
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<div class="note-title"> Cloning</div>
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 +
<div id="content_note" >
 +
 +
<b>Results</b></br></br>
 +
 +
<div align="center"><img id="figure" alt="0818Fig1" src="http://2012.igem.org/wiki/images/8/87/121003-2.PNG"></img></div>
 +
<div style="clear:both;"></div>
 +
 +
<div align="center"><img id="figure" alt="0818Fig1" src="http://2012.igem.org/wiki/images/8/8a/121003-3.PNG"></img></div>
 +
<div style="clear:both;"></div>
</div>
</div>
 +
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="http://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="http://2012.igem.org/wiki/images/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
<a id="back_to_the_calender" style="font-size:12px;color:#111;text-decoration:none;float:right;margin:20px 0px 0px 5px;" href="http://2012.igem.org/wiki/index.php?title=Team:KAIST_Korea/Notebook_Labnote"><img src="http://2012.igem.org/wiki/images/e/e3/KAIST_calendar_icon.png" style="width:60px;height:60px;display:none;"/>Back to the Calendar</a>
</section>
</section>

Revision as of 01:11, 27 October 2012

KAIST Korea 2012 iGEM

Notebook : Labnote-October

Labnote

October

October 1st 2012

No Special Event!
Back to the Calendar
October 2nd 2012

 Flip Flop

bFMO template preparation with new primer
Results

0818Fig1

We have found that our bFMO sequence was wrong. So that we have ordered new primers with right sequence.
Back to the Calendar
October 3rd 2012

 Flip Flop

Visualization of inversion from GFP to RFP
Procedure


bfmo gene is constructed in the vector(pJ401, Km registance) and transformed into DH5α.
50λ cell from stock is inoculated in 5mL LB


Visualization of inversion from GFP to RFP
Procedure

We sampled the cell every 3hr. We induced one group of samples and un-induced another group of samples as a control. We took the picture of cell with confocal microscope at absorbance 500nm for GFP expression and 600nm for RFP expression.
Results

0818Fig1


Discussion

We obtained proper data. Un-induced sample doesn’t show measurable RFP expression throughout the culture. However, induced sample shows the increase of RFP expression. Increase of GFP level in induced sample is due to the stability of GFP and time delay before inversion.


Insert DNA preparation with OE pcr
Results

0818Fig1


Cloning
Results

0818Fig1
0818Fig1
Back to the Calendar
October 4th 2012

No Special Event!
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October 5th 2012

No Special Event!
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October 6th 2012

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October 7th 2012

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October 8th 2012

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October 9th 2012

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October 10th 2012

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October 11th 2012

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October 12th 2012

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October 13th 2012

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October 14th 2012

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October 15th 2012

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October 16th 2012

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October 17th 2012

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October 18th 2012

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October 19th 2012

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October 20th 2012

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October 21st 2012

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October 22nd 2012

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October 23rd 2012

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October 24th 2012

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October 25th 2012

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October 26th 2012

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October 27th 2012

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October 28th 2012

No Special Event!
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October 29th 2012

No Special Event!
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October 30th 2012

No Special Event!
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October 31st 2012

No Special Event!
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