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Team:IvyTech-South Bend/Notebook
From 2012.igem.org
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Oops Event!!! Protocol correction due to low yield or transformation. Killswitch transformation was redone (serial dilution test for transformation efficiency) Kamamiosin proves to be better selection for transformation for the psb1Ak3 plasmid backbone. | Oops Event!!! Protocol correction due to low yield or transformation. Killswitch transformation was redone (serial dilution test for transformation efficiency) Kamamiosin proves to be better selection for transformation for the psb1Ak3 plasmid backbone. | ||
| - | -Pulled K190015 because its an arsenic sensitive promoter ẅ RFP | + | -Pulled K190015 because its an arsenic sensitive promoter ẅ RFP |
| - | -Transformed K190015 on Psb1A13 | + | -Transformed K190015 on Psb1A13 |
July 16, 2012 Week 5. | July 16, 2012 Week 5. | ||
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Redid transformation on electrocompetent eColi cells. Results yielde pink & white colonies. Expanded the colonies and purfied K190015 | Redid transformation on electrocompetent eColi cells. Results yielde pink & white colonies. Expanded the colonies and purfied K190015 | ||
| - | -PINK Hypothesized to be succesful transformation with Rtt | + | -PINK Hypothesized to be succesful transformation with Rtt |
| - | -WHITE Unsure, proceeded to test in wk. 7 | + | -WHITE Unsure, proceeded to test in wk. 7 |
August 1, 2012 Week 7 | August 1, 2012 Week 7 | ||
To test sensitivity of k190015; including pink and white colonies, ran 96 well and stimulating with dilute Arsenic sol. Results were inconclusive. | To test sensitivity of k190015; including pink and white colonies, ran 96 well and stimulating with dilute Arsenic sol. Results were inconclusive. | ||
Revision as of 00:28, 4 October 2012
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<a href="#">Lab Notebook: See our weekly lab work</a>
June 18, 2012 Week 1. Staging and presentation of project storming to Ivy Tech advisory board. Parts research and descriptions.
June 25, 2012 Week 2. Continuation of parts research including promoters & killswitch screening and selction. Constitutive PTeT promoter, RBS, double terminator.
July 2, 2012 Week 3. Checked cultures and paired plates. Continuation of parts research and construction
July 9, 2012 Week 4. Oops Event!!! Protocol correction due to low yield or transformation. Killswitch transformation was redone (serial dilution test for transformation efficiency) Kamamiosin proves to be better selection for transformation for the psb1Ak3 plasmid backbone.
-Pulled K190015 because its an arsenic sensitive promoter ẅ RFP
-Transformed K190015 on Psb1A13
July 16, 2012 Week 5. Propped Test promoter and transferred to Arsenic inducible promoter J33201 with Ars R repressor binding site. To be able to test for decreasing sensitivity to Arsenic.
July 23, 2012 Week 6. Redid transformation on electrocompetent eColi cells. Results yielde pink & white colonies. Expanded the colonies and purfied K190015
-PINK Hypothesized to be succesful transformation with Rtt
-WHITE Unsure, proceeded to test in wk. 7
August 1, 2012 Week 7 To test sensitivity of k190015; including pink and white colonies, ran 96 well and stimulating with dilute Arsenic sol. Results were inconclusive.
