Team:IvyTech-South Bend/Notebook

From 2012.igem.org

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July 9, 2012  Week 4.
July 9, 2012  Week 4.
Oops Event!!!  Protocol correction due to low yield or transformation.  Killswitch transformation was redone (serial dilution test for transformation efficiency) Kamamiosin proves to be better selection for transformation for the psb1Ak3 plasmid backbone.
Oops Event!!!  Protocol correction due to low yield or transformation.  Killswitch transformation was redone (serial dilution test for transformation efficiency) Kamamiosin proves to be better selection for transformation for the psb1Ak3 plasmid backbone.
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-Pulled K190015 because its an arsenic sensitive promoter ẅ RFP
 
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-Pulled K190015 because its an arsenic sensitive promoter ẅ RFP
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Revision as of 00:02, 4 October 2012


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Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions


<a href="#">Lab Notebook: See our weekly lab work</a>

June 18, 2012 Week 1. Staging and presentation of project storming to Ivy Tech advisory board. Parts research and descriptions.

June 25, 2012 Week 2. Continuation of parts research including promoters & killswitch screening and selction. Constitutive PTeT promoter, RBS, double terminator.

July 2, 2012 Week 3. Checked cultures and paired plates. Continuation of parts research and construction

July 9, 2012 Week 4. Oops Event!!! Protocol correction due to low yield or transformation. Killswitch transformation was redone (serial dilution test for transformation efficiency) Kamamiosin proves to be better selection for transformation for the psb1Ak3 plasmid backbone.

-Pulled K190015 because its an arsenic sensitive promoter ẅ RFP

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