Team:HokkaidoU Japan/Notebook/plastic Week 6

From 2012.igem.org

(Difference between revisions)
(DNA sequencing)
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@Taguchi lab
@Taguchi lab
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#sample1 JM109 culture from July18th 1.4643g , 0.0054 (tube+polymer , polymer)
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#sample1 JM109 culture from July 18th 1.4643 g , 0.0054 (tube+polymer , polymer)
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#sample2 JM109 culture from July18th 1.4642g , 0.0040
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#sample2 JM109 culture from July 18th 1.4642 g , 0.0040
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#sample3 DH5a                       1.4666g , 0.0056
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#sample3 DH5a                         1.4666 g , 0.0056
==Preculture==
==Preculture==
    
    
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Inoculated from three different plates(Name it 1,2,3)<br />
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Inoculated from three different plates (Name it 1,2,3).<br />
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Cultured in media of LB 150microL+Amp1.5microL<br />
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Cultured in media of LB 150 ul + 1.5 ul of Amp.<br />
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30℃ 20:00-7th10:00<br />
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30C  August 6th 20:00 ~ 7th 10:00<br />
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<p>
<p>
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We made 8 sapmles to see if Panthotenic acid is nessesary for plastic growth. <br>
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We made 8 samples to see if Panthotenic acid is necessary for plastic growth. <br />
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Panthotenic acid (+)1.5ml 4samples <br />
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Panthotenic acid (+)1.5 ml 4 samples <br />
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Panthotenic acid (+)0.7ml 2samples <br />
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Panthotenic acid (+)0.7 ml 2 samples <br />
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Panthotenic acid (-)0.7ml 2samples <br />
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Panthotenic acid (-)0.7 ml 2 samples <br />
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Started liquid culture from Aug 7th 11:00 until Aug 9th 09:50.<br>
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Incubated liquid culture from August 7th 11:00 until 9th 09:50.<br />
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==Harvest==
==Harvest==
<p>
<p>
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We did the protocol of Harvest on 8 samples.
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We did the protocol of harvest on 8 samples.
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</p>
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Revision as of 15:42, 26 September 2012

Contents

August 6th

Polymer extract

@Taguchi lab

  1. sample1 JM109 culture from July 18th 1.4643 g , 0.0054 (tube+polymer , polymer)
  2. sample2 JM109 culture from July 18th 1.4642 g , 0.0040
  3. sample3 DH5a 1.4666 g , 0.0056

Preculture

Inoculated from three different plates (Name it 1,2,3).
Cultured in media of LB 150 ul + 1.5 ul of Amp.
30C August 6th 20:00 ~ 7th 10:00


August 7th

Liquid culture

We made 8 samples to see if Panthotenic acid is necessary for plastic growth.
Panthotenic acid (+)1.5 ml 4 samples
Panthotenic acid (+)0.7 ml 2 samples
Panthotenic acid (-)0.7 ml 2 samples
Incubated liquid culture from August 7th 11:00 until 9th 09:50.


August 9th

@Taguchi lab

Harvest

We did the protocol of harvest on 8 samples.


August 10th

Plasmid extraction

The plasmid of BBa_K342001(PhaC) was extracted by FastGene Plasmid Mini Kit(Nippon Genetics) and we got 50 ul of DNA solutions.


August 11th

Sequencing

BBa_K342001(PhaC) was seqenced.