Team:HokkaidoU Japan/Notebook/plastic Week 6
From 2012.igem.org
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@Taguchi lab | @Taguchi lab | ||
- | #sample1 JM109 culture from | + | #sample1 JM109 culture from July 18th 1.4643 g , 0.0054 (tube+polymer , polymer) |
- | #sample2 JM109 culture from | + | #sample2 JM109 culture from July 18th 1.4642 g , 0.0040 |
- | #sample3 DH5a | + | #sample3 DH5a 1.4666 g , 0.0056 |
==Preculture== | ==Preculture== | ||
- | Inoculated from three different plates(Name it 1,2,3)<br /> | + | Inoculated from three different plates (Name it 1,2,3).<br /> |
- | Cultured in media of LB | + | Cultured in media of LB 150 ul + 1.5 ul of Amp.<br /> |
- | + | 30C August 6th 20:00 ~ 7th 10:00<br /> | |
</p> | </p> | ||
</div></div> | </div></div> | ||
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<p> | <p> | ||
- | We made 8 | + | We made 8 samples to see if Panthotenic acid is necessary for plastic growth. <br /> |
- | Panthotenic acid (+)1. | + | Panthotenic acid (+)1.5 ml 4 samples <br /> |
- | Panthotenic acid (+)0. | + | Panthotenic acid (+)0.7 ml 2 samples <br /> |
- | Panthotenic acid (-)0. | + | Panthotenic acid (-)0.7 ml 2 samples <br /> |
- | + | Incubated liquid culture from August 7th 11:00 until 9th 09:50.<br /> | |
</p> | </p> | ||
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==Harvest== | ==Harvest== | ||
<p> | <p> | ||
- | We did the protocol of | + | We did the protocol of harvest on 8 samples. |
</p> | </p> | ||
</div></div> | </div></div> |
Revision as of 15:42, 26 September 2012
Contents |
August 6th
Polymer extract
@Taguchi lab
- sample1 JM109 culture from July 18th 1.4643 g , 0.0054 (tube+polymer , polymer)
- sample2 JM109 culture from July 18th 1.4642 g , 0.0040
- sample3 DH5a 1.4666 g , 0.0056
Preculture
Inoculated from three different plates (Name it 1,2,3).
Cultured in media of LB 150 ul + 1.5 ul of Amp.
30C August 6th 20:00 ~ 7th 10:00
August 7th
Liquid culture
We made 8 samples to see if Panthotenic acid is necessary for plastic growth.
Panthotenic acid (+)1.5 ml 4 samples
Panthotenic acid (+)0.7 ml 2 samples
Panthotenic acid (-)0.7 ml 2 samples
Incubated liquid culture from August 7th 11:00 until 9th 09:50.
August 9th
@Taguchi lab
Harvest
We did the protocol of harvest on 8 samples.
August 10th
Plasmid extraction
The plasmid of BBa_K342001(PhaC) was extracted by FastGene Plasmid Mini Kit(Nippon Genetics) and we got 50 ul of DNA solutions.
August 11th
Sequencing
BBa_K342001(PhaC) was seqenced.