Team:HokkaidoU Japan/Notebook/plastic Week 5

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Contents

August 2nd

Transformation

Transformation of pGEM into BL21. This transformation is the plastic production test in BL21.

  1. Added 1ul of plasmid DNA to 50ul of thawed competent cells on ice.
  2. Incubated on ice for 30min.
  3. Added 600ul of LB.
  4. Prepared and Labeled two petri dishes with LBA.
  5. Plate 300ul of the transformation onto LBA dish and spread.
  6. Added 900ul of LB to 100ul of the transformation and plated 300ul of it onto LBA dish then spread.
  7. Incubated the plates at 37C for OOhrs.

August 3rd

Liquid culture

Liquid culture of Transformed BL21(DE3)


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