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Team:HokkaidoU Japan/Notebook/aggregation Week 4 - Revision history
2024-03-29T01:29:11Z
Revision history for this page on the wiki
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TaKeZo: /* Electrophoresis */
2012-09-27T03:18:05Z
<p><span class="autocomment">Electrophoresis</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 03:18, 27 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===July 24th===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===July 24th===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="hokkaidou-section"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="hokkaidou-section"></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">=</del>=====Electrophoresis<del class="diffchange diffchange-inline">=</del>=====</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>=====Electrophoresis=====</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Electrophoresis for Ag43(plasmid extraction at July 23rd) and Ag43 digestion results(digested with EcoRI and SpeI)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Electrophoresis for Ag43(plasmid extraction at July 23rd) and Ag43 digestion results(digested with EcoRI and SpeI)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120724 ag43-dt pcr.jpg|thumb|Plasmid extraction result]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120724 ag43-dt pcr.jpg|thumb|Plasmid extraction result]]</div></td></tr>
</table>
TaKeZo
http://2012.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/aggregation_Week_4&diff=234239&oldid=prev
Haruka.O at 02:48, 27 September 2012
2012-09-27T02:48:02Z
<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Plasmid extraction====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====Plasmid extraction====</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Plasmid extraction for Ag43(resuspended colony incubated at July 17th and resuspended colony incubated at July 20th). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Plasmid extraction for Ag43(resuspended colony incubated at July 17th and resuspended colony incubated at July 20th). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br style="line-height: 0; clear: both;" /></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> |10 ul</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> |10 ul</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> |}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> |}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br style="line-height: 0; clear: both;" /></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|Hold</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|Hold</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br style="line-height: 0; clear: both;" /></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120726 digestion ligation.jpg|thumb|Digestion and Ligation results]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120726 digestion ligation.jpg|thumb|Digestion and Ligation results]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are no band in the lane of ligation products. But if digestion products were not ligated, two bands of digestion products would exist in the lane. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There are no band in the lane of ligation products. But if digestion products were not ligated, two bands of digestion products would exist in the lane. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br style="line-height: 0; clear: both;" /></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|Hold</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|Hold</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br style="line-height: 0; clear: both;" /></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|Hold</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|Hold</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br style="line-height: 0; clear: both;" /></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>I could not understand what is happend.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>I could not understand what is happend.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>I tried it again, but the result was the same.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>I tried it again, but the result was the same.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br style="line-height: 0; clear: both;" /></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>
Haruka.O
http://2012.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/aggregation_Week_4&diff=231990&oldid=prev
TaKeZo at 02:03, 27 September 2012
2012-09-27T02:03:03Z
<p></p>
<a href="http://2012.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/aggregation_Week_4&diff=231990&oldid=213557">Show changes</a>
TaKeZo
http://2012.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/aggregation_Week_4&diff=213557&oldid=prev
Slecat at 20:00, 26 September 2012
2012-09-26T20:00:52Z
<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">Revision as of 20:00, 26 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digestion to confirm how many PstI cutting sites are there in K346007 and for Ag43-dT complex with SpeI and XbaI.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digestion to confirm how many PstI cutting sites are there in K346007 and for Ag43-dT complex with SpeI and XbaI.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br <ins class="diffchange diffchange-inline">/</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ag43</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ag43</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PstI</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PstI</div></td></tr>
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<td colspan="2" class="diff-lineno">Line 75:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Ag43-dT<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Ag43-dT<br <ins class="diffchange diffchange-inline">/</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>SpeI and XbaI</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>SpeI and XbaI</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> {|class="hokkaidou-table-digestion"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> {|class="hokkaidou-table-digestion"</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 340:</td>
<td colspan="2" class="diff-lineno">Line 340:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digestion of pT7-RBS on pSB1K3 with SpeI and Ag43-dT on pSB1AK3(cut with SpeI & XbaI) with HindIII.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digestion of pT7-RBS on pSB1K3 with SpeI and Ag43-dT on pSB1AK3(cut with SpeI & XbaI) with HindIII.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br <ins class="diffchange diffchange-inline">/</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pT7-RBS on pSB1K3</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pT7-RBS on pSB1K3</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> {|class="hokkaidou-table-digestion"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> {|class="hokkaidou-table-digestion"</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 453:</td>
<td colspan="2" class="diff-lineno">Line 453:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Plasmid extraction products(pT7-RBS-Ag43-dT)<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Plasmid extraction products(pT7-RBS-Ag43-dT)<br <ins class="diffchange diffchange-inline">/</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PstI</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>PstI</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> {|class="hokkaidou-table-digestion"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> {|class="hokkaidou-table-digestion"</div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 474:</td>
<td colspan="2" class="diff-lineno">Line 474:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Plasmid extraction products(pT7-RBS-Ag43-dT)<br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Plasmid extraction products(pT7-RBS-Ag43-dT)<br <ins class="diffchange diffchange-inline">/</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>EcoR1 and Spe1</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>EcoR1 and Spe1</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> {|class="hokkaidou-table-digestion"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> {|class="hokkaidou-table-digestion"</div></td></tr>
</table>
Slecat
http://2012.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/aggregation_Week_4&diff=208112&oldid=prev
Slecat at 18:06, 26 September 2012
2012-09-26T18:06:03Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 18:06, 26 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Plasmid extraction==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Plasmid extraction==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Mini-prep </del>for Ag43(resuspended colony incubated at July 17th and resuspended colony incubated at July 20th). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Plasmid extraction </ins>for Ag43(resuspended colony incubated at July 17th and resuspended colony incubated at July 20th). We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Electrophoresis==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Electrophoresis==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Electrophoresis for Ag43(<del class="diffchange diffchange-inline">mini-preped </del>at July 23rd) and Ag43 digestion results(digested with EcoRI and SpeI)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Electrophoresis for Ag43(<ins class="diffchange diffchange-inline">plasmid extraction </ins>at July 23rd) and Ag43 digestion results(digested with EcoRI and SpeI)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120724 ag43-dt pcr.jpg|thumb|<del class="diffchange diffchange-inline">Mini-prep </del>result]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120724 ag43-dt pcr.jpg|thumb|<ins class="diffchange diffchange-inline">Plasmid extraction </ins>result]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120724 Ag43(K346007 cut with E&amp;S) digestion product.jpg|thumb|Digestion result]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120724 Ag43(K346007 cut with E&amp;S) digestion product.jpg|thumb|Digestion result]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Plasmid extraction for 5 cultures of pT7-RBS-Ag43-dT. We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Plasmid extraction for 5 cultures of pT7-RBS-Ag43-dT. We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012_120729_pt7-rbs-ag43-dt.jpg|thumb|<del class="diffchange diffchange-inline">mini-prep </del>products]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012_120729_pt7-rbs-ag43-dt.jpg|thumb|<ins class="diffchange diffchange-inline">Plasmid extraction </ins>products]]</div></td></tr>
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</table>
Slecat
http://2012.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/aggregation_Week_4&diff=203056&oldid=prev
Kwt at 16:01, 26 September 2012
2012-09-26T16:01:59Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 16:01, 26 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Transformation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Transformation==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Transformation <del class="diffchange diffchange-inline">of </del>plasmid DNA ligated at 25th (pT7-RBS-Ag43-dT on pSB1K3) <del class="diffchange diffchange-inline">in </del>BL21.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Transformation plasmid DNA ligated at 25th (pT7-RBS-Ag43-dT on pSB1K3) <ins class="diffchange diffchange-inline">into </ins>BL21.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Added 2 ul of plasmid DNA to 50 ul of thawed competent cells on ice.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Added 2 ul of plasmid DNA to 50 ul of thawed competent cells on ice.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubated on ice for 30 min.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubated on ice for 30 min.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Added 600 ul of LB then incubated the cells for 2 hrs at 37C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Added 600 ul of LB then incubated the cells for 2 hrs at 37C.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Prepared and Labeled three petri dishes with LBK X2 and LBC.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Prepared and Labeled three petri dishes with LBK X2 and LBC.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#<del class="diffchange diffchange-inline">Plate </del>300 ul of the transformation onto LBK dish <del class="diffchange diffchange-inline">and spread</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">Spread </ins>300 ul of the transformation onto LBK dish.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#Added 900 ul of LB to 100 ul of the transformation and <del class="diffchange diffchange-inline">plated </del>300 ul of it onto LBC dish and LBK dish <del class="diffchange diffchange-inline">then spread</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#Added 900 ul of LB to 100 ul of the transformation and <ins class="diffchange diffchange-inline">spread </ins>300 ul of it onto LBC dish and LBK dish. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubated the plates at 37C for 17 hrs and 30 min.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubated the plates at 37C for 17 hrs and 30 min.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digestion of pT7-RBS on pSB1K3 with SpeI and Ag43-dT on pSB1AK3(cut with SpeI & XbaI) with HindIII.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Digestion of pT7-RBS on pSB1K3 with SpeI and Ag43-dT on pSB1AK3(cut with SpeI & XbaI) with HindIII.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pT7-RBS on pSB1K3</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pT7-RBS on pSB1K3</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> {|class="hokkaidou-table-digestion"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> {|class="hokkaidou-table-digestion"</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> |}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> |}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Digestioned </del>at 37C for <del class="diffchange diffchange-inline">2hrs</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Digested </ins>at 37C for <ins class="diffchange diffchange-inline">2 hrs</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120728 Digestion pT7-RBS on pSB1K3(SpeI) Ag43-dT on pSB1AK3(HindIII).jpg|thumb|digestion result]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120728 Digestion pT7-RBS on pSB1K3(SpeI) Ag43-dT on pSB1AK3(HindIII).jpg|thumb|digestion result]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>This results <del class="diffchange diffchange-inline">confirmed </del>that pSB1AK3 was successfully digested into fragments(1.3K and 1.8K bp), but we <del class="diffchange diffchange-inline">weren</del>'t <del class="diffchange diffchange-inline">confirmed </del>whether pT7-RBS on pSB1K3 was digested or not.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>This results <ins class="diffchange diffchange-inline">showed </ins>that pSB1AK3 was successfully digested into fragments(1.3K and 1.8K bp), but we <ins class="diffchange diffchange-inline">couldn</ins>'t <ins class="diffchange diffchange-inline">confirm </ins>whether pT7-RBS on pSB1K3 was digested or not.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Gel extraction==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Gel extraction==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Gel extraction for digestion production. We used FastGene Gel&PCR extraction kit(NipponGenetics)and got 50 ul of DNA solution. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Gel extraction for digestion production. We used FastGene Gel&PCR extraction kit(NipponGenetics) and got 50 ul of DNA solution. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Ethanol precipitation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Ethanol precipitation==</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Ligation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Ligation==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Ligation <del class="diffchange diffchange-inline">for </del>pT7-RBS on pSB1K3(Vector) and Ag43-dT(Insert)</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Ligation <ins class="diffchange diffchange-inline">of </ins>pT7-RBS on pSB1K3(Vector) and Ag43-dT(Insert)</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="hokkaidou-table-ligation"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|class="hokkaidou-table-ligation"</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Plasmid extraction==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Plasmid extraction==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Mini-prep </del>for <del class="diffchange diffchange-inline">five </del>cultures of pT7-RBS-Ag43-dT. We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Plasmid extraction </ins>for <ins class="diffchange diffchange-inline">5 </ins>cultures of pT7-RBS-Ag43-dT. We used FastGene Plasmid Mini Kit(Nippon Genetics) and got 50 ul of DNA solutions. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012_120729_pt7-rbs-ag43-dt.jpg|thumb|mini-prep products]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012_120729_pt7-rbs-ag43-dt.jpg|thumb|mini-prep products]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>
Kwt
http://2012.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/aggregation_Week_4&diff=202588&oldid=prev
Kwt: /* Electrophoresis */
2012-09-26T15:49:30Z
<p><span class="autocomment">Electrophoresis</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 15:49, 26 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Placed TBE agarose gel in Electrophoresis chamber.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Placed TBE agarose gel in Electrophoresis chamber.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Added 1/2X TBE buffer to Electrophoresis chamber.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Added 1/2X TBE buffer to Electrophoresis chamber.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#Added 5 ul of <del class="diffchange diffchange-inline">Etbr </del>and ran at 100 V for 30 min. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#Added 5 ul of <ins class="diffchange diffchange-inline">EtBr </ins>and ran at 100 V for 30 min. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Apply 1kb DNA ladder and each samples.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Apply 1kb DNA ladder and each samples.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Ran at 100 V for 30 min.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Ran at 100 V for 30 min.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120726 digestion ligation.jpg|thumb|Digestion and Ligation results]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120726 digestion ligation.jpg|thumb|Digestion and Ligation results]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>There are no band in the lane of ligation products. But if digestion products <del class="diffchange diffchange-inline">didn't ligate</del>, <del class="diffchange diffchange-inline">there are </del>two bands of digestion products would exist in the lane. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>There are no band in the lane of ligation products. But if digestion products <ins class="diffchange diffchange-inline">were not ligated</ins>, two bands of digestion products would exist in the lane. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="hokkaidou-notebook-daily"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div class="hokkaidou-notebook-daily"></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==July 27th==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==July 27th==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div></div></td></tr>
</table>
Kwt
http://2012.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/aggregation_Week_4&diff=202364&oldid=prev
Kwt: /* Transformation */
2012-09-26T15:43:46Z
<p><span class="autocomment">Transformation</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 15:43, 26 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Transformation==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Transformation==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Transformation <del class="diffchange diffchange-inline">of </del>plasmid DNA ligated at 25th (pT7-RBS-Ag43-dT on pSB1K3) <del class="diffchange diffchange-inline">in </del>BL21.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Transformation plasmid DNA ligated at 25th (pT7-RBS-Ag43-dT on pSB1K3) <ins class="diffchange diffchange-inline">into </ins>BL21.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Added 2 ul of plasmid DNA to 50 ul of thawed competent cells on ice.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Added 2 ul of plasmid DNA to 50 ul of thawed competent cells on ice.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubated on ice for 30 min.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubated on ice for 30 min.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Added 200 ul of LB then incubated the cells for 2 hrs at 37C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Added 200 ul of LB then incubated the cells for 2 hrs at 37C.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Prepared and Labeled two petri dishes with LBK.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Prepared and Labeled two petri dishes with LBK.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#<del class="diffchange diffchange-inline">Plate </del>200 ul of the transformation onto first dish <del class="diffchange diffchange-inline">and spread</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#<ins class="diffchange diffchange-inline">Spread </ins>200 ul of the transformation onto first dish.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#Added 450 ul of LB to 50 ul of the transformation and <del class="diffchange diffchange-inline">plated </del>200 ul of it onto second dish <del class="diffchange diffchange-inline">and spread</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#Added 450 ul of LB to 50 ul of the transformation and <ins class="diffchange diffchange-inline">spread </ins>200 ul of it onto second dish. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubated the plates at 37C for over 30 hrs.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Incubated the plates at 37C for over 30 hrs.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>
Kwt
http://2012.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/aggregation_Week_4&diff=201892&oldid=prev
Kwt: /* Ethanol precipitation */
2012-09-26T15:32:31Z
<p><span class="autocomment">Ethanol precipitation</span></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 15:32, 26 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ethanol precipitation of digestion and gel extraction products.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Ethanol precipitation of digestion and gel extraction products.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Added 5 ul of NaoAc, 1.5 ul of glycogen and 125 ul of 100% ethanol.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Added 5 ul of NaoAc, 1.5 ul of glycogen and 125 ul of 100% ethanol.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#Centrifuged at 15000 rpm, <del class="diffchange diffchange-inline">10min </del>at 4C.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#Centrifuged at 15000 rpm, <ins class="diffchange diffchange-inline">10 min </ins>at 4C.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Removed supernatant and added 220 ul of 70% ethanol.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Removed supernatant and added 220 ul of 70% ethanol.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Centrifuged at 15000 rpm, 5 min at 4C.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>#Centrifuged at 15000 rpm, 5 min at 4C.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>#Removed supernatant and <del class="diffchange diffchange-inline">air drying </del>at room temperature <del class="diffchange diffchange-inline">then </del>added 5 ul of DW. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>#Removed supernatant and <ins class="diffchange diffchange-inline">dried out </ins>at room temperature <ins class="diffchange diffchange-inline">after that </ins>added 5 ul of DW. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>
Kwt
http://2012.igem.org/wiki/index.php?title=Team:HokkaidoU_Japan/Notebook/aggregation_Week_4&diff=201715&oldid=prev
Kwt: /* Digestion */
2012-09-26T15:27:26Z
<p><span class="autocomment">Digestion</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 15:27, 26 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Digestion==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==Digestion==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Digestion of pT7-RBS on pSB1K3 <del class="diffchange diffchange-inline">digested </del>with SpeI.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Digestion of pT7-RBS on pSB1K3 with SpeI.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120725 Digestion Ag43-dt on pSB1AK3(HindIII) &amp; pT7-RBS on pSB1K3(SpeI).jpg|thumb|Digestion result(Ag43-dT and pT7-RBS)]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[image:HokkaidoU2012 120725 Digestion Ag43-dt on pSB1AK3(HindIII) &amp; pT7-RBS on pSB1K3(SpeI).jpg|thumb|Digestion result(Ag43-dT and pT7-RBS)]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We were confirmed that <del class="diffchange diffchange-inline">pAB1AK3 </del>was digested and became 1.3k and 1.8k bp fragments by HindIII. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We were confirmed that <ins class="diffchange diffchange-inline">pSB1AK3 </ins>was digested and became 1.3k and 1.8k bp fragments by HindIII. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>
Kwt