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Team:HokkaidoU Japan/Notebook
From 2012.igem.org
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| Line 67: | Line 67: | ||
=Ag43&Lysis team experiment= | =Ag43&Lysis team experiment= | ||
==July== | ==July== | ||
| - | ==4th== | + | ===4th=== |
;Transformation | ;Transformation | ||
#Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43) in DH5α | #Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43) in DH5α | ||
#Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43) . pT7 was 21hrs and Others were 20hrs cultivated | #Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43) . pT7 was 21hrs and Others were 20hrs cultivated | ||
| - | ==5th== | + | ===5th=== |
;Transformation | ;Transformation | ||
K346007(Ag43) was failed to cultivate on LBC plate. | K346007(Ag43) was failed to cultivate on LBC plate. | ||
| Line 80: | Line 80: | ||
#Single colony isolation of BBa_B0015, B0034, I179005 and K542009. | #Single colony isolation of BBa_B0015, B0034, I179005 and K542009. | ||
#Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins | #Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins | ||
| - | + | BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.''' | |
| - | ==6th== | + | ===6th=== |
;Liquid culture | ;Liquid culture | ||
#Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43) | #Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43) | ||
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#Single colony isolation of K346007(Ag43). | #Single colony isolation of K346007(Ag43). | ||
| - | ==7th== | + | ===7th=== |
;mini-prep | ;mini-prep | ||
#mini-prep of dT,RBS,pT7 and pLacI-RBS-Ag43. | #mini-prep of dT,RBS,pT7 and pLacI-RBS-Ag43. | ||
;Glycerol stock | ;Glycerol stock | ||
Revision as of 02:40, 7 July 2012
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions |
|---|
Contents |
Hello
We are team HokkaidoU Japan! Today we learn and start to edit wiki.
(>ω<)
Dear Mr.Ortiz, I saw the help page which you edited.
hola!
March
Spring Boot Camp
- date
- March 5 (Mon) ~ March 9 (Fri)
Monday, March 5
- Session #1
- Short lecture about moleculer biology (Mr.Yamazaki, our adviser)
- Session #2
- Tutrial: How to use 'Unipro UGENE' (iTakeshi)
- Session #3
- Guidance: Wiki Reading (Laury)
- Example: 2010 MIT
Tuesday, March 6
- Session #4~6
- Reading Wikis in turn and discussions
- 2010 NYU
- 2009 Cambridge
- 2009 Growningen
Wednesday, March 7
- Session #7~11
- Reading Wikis (2)
- 2010 Washington
- 2009 Valencia
- 2011 Barklay
- 2010 Paris
- 2010 Bristol
Thursday, March 8
- Session #12
- 2012 Project Brainstorming
- The details is secret! :)
- Session #13
- Guidance: How to read papers (Laury)
Friday, March 9
- Session #14
- 2012 Project Brainstorming (2)
- Session #15
- Guidance: How to look up papers you want (Laury)
- Session #16
- Tutorial: Modeling the behavior of cells (iTakeshi)
- Session #17
- Final Session: Reviewing this camp
- Party!!
Ag43&Lysis team experiment
July
4th
- Transformation
- Transformation of BBa_B0015(dT), B0034(RBS), I179005(pT7), K346007(Ag43), and K542009(pLacI-RBS-Ag43) in DH5α
- Cultivated on LBA(dt,RBS,T7) and LBC(Ag43, pLacI-RBS-Ag43) . pT7 was 21hrs and Others were 20hrs cultivated
5th
- Transformation
K346007(Ag43) was failed to cultivate on LBC plate.
- Transformation of K346007(Ag43) in DH5α.
- Cultivated on LBC in 21hrs.
- Single colony isolation
- Single colony isolation of BBa_B0015, B0034, I179005 and K542009.
- Cultivation on LBA(dt,RBS,T7) and LBC(pLacI-RBS-Ag43) in 14hrs30mins
BBa_K542009 was Ag43 only part! And the part didn't have Biobrick suffix.
6th
- Liquid culture
- Liquid culture in LBA(dT,RBS,pT7) and LBC(pLacI-RBS-Ag43)
- LB 1ml is for glycerol stocks and 2ml is for mini-prep.
- 16hrs Cultivation
- Single colony isolation
- Single colony isolation of K346007(Ag43).
7th
- mini-prep
- mini-prep of dT,RBS,pT7 and pLacI-RBS-Ag43.
- Glycerol stock
