Team:HKUST-Hong Kong/Glossary

From 2012.igem.org

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           <p><strong>Signal peptide</strong>&nbsp;&nbsp;transient &lsquo;zip code&rsquo; of premature protein which facilitates the secretion of protein in <em>Bacillus subtilis</em>.</p>
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           <p><strong>Signal peptide</strong> – transient &lsquo;zip code&rsquo; of premature protein which facilitates the secretion of protein in <em>Bacillus subtilis</em>.</p>
<p><strong>YbdN</strong> – Signal peptide of protein YbdN in <em>Bacillus subtilis.</em></p>
<p><strong>YbdN</strong> – Signal peptide of protein YbdN in <em>Bacillus subtilis.</em></p>
<p><strong>ybdN</strong> – Gene that codes for YbdN.</p>
<p><strong>ybdN</strong> – Gene that codes for YbdN.</p>
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<p><strong>(EAAAK)<sub>n</sub> type helical linker</strong> – Stiff, long helical linker designed to separate fusion  proteins with minimal disturbance to the function of both proteins. </p>
<p><strong>(EAAAK)<sub>n</sub> type helical linker</strong> – Stiff, long helical linker designed to separate fusion  proteins with minimal disturbance to the function of both proteins. </p>
<p><strong>RPMrel</strong> – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for HT29 colon carcinoma cells.</p>
<p><strong>RPMrel</strong> – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for HT29 colon carcinoma cells.</p>
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<p><strong>FLAG™</strong> &nbsp; &nbsp; 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.</p>
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<p><strong>FLAG™</strong> – 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.</p>
<p><strong><em>PxylA</em></strong> – The region containing the sigma A  binding region for the xylose inducible promoter.</p>
<p><strong><em>PxylA</em></strong> – The region containing the sigma A  binding region for the xylose inducible promoter.</p>
<p><strong>XylR</strong> – The transcriptional regulator for  PxylA promoter.</p>
<p><strong>XylR</strong> – The transcriptional regulator for  PxylA promoter.</p>

Revision as of 15:36, 26 September 2012

Team:HKUST-Hong Kong - 2012.igem.org

Glossary

Signal peptide – transient ‘zip code’ of premature protein which facilitates the secretion of protein in Bacillus subtilis.

YbdN – Signal peptide of protein YbdN in Bacillus subtilis.

ybdN – Gene that codes for YbdN.

YdjM – Signal peptide from protein YdjM in Bacillus subtilis.

ydjM – Gene that codes for YdjM.

BMP2 – Bone morphogenetic protein 2.

LytCB. subtilis inherent cell-wall associated hydrolase mediating cell-wall turnover.

lytC – Gene that codes for the LytC hydrolase.

(EAAAK)n type helical linker – Stiff, long helical linker designed to separate fusion proteins with minimal disturbance to the function of both proteins.

RPMrel – A nine amino acid disulfide-constrained peptide with the amino acid sequence n-CPIEDRPMC-c. Product of screening to isolate peptides with positive affinity for HT29 colon carcinoma cells.

FLAG™ – 8 amino acid peptide tag trademarked by the Sigma-Aldrich Corp. used in attachment to the protein-of-interest to facilitate multiple assays.

PxylA – The region containing the sigma A binding region for the xylose inducible promoter.

XylR – The transcriptional regulator for PxylA promoter.

pTms – A low efficient constitutive promoter in E. coli and B. subtilis.

EndoA – Endoribonuclease that can inactivate cellular mRNAs by cleaving them at specific but frequently occurring sites (i.e. UAUAAU↓AC).

ydcE (EndoA) – Gene that codes for the EndoA endoribonuclease.

YdcD – Antidote that counteracts the function of EndoA.

ydcD (endB) – Gene that codes for the YdcD antidote.