Team:HKUST-Hong Kong/Achievement

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Team:HKUST-Hong Kong - 2012.igem.org

ACHIEVEMENT

From the day when our project was first proposed in mid-March to the day when we stop wet lab work in mid-September, we, our 2012 HKUST iGEM team have been working on our project for almost six months. In our six-month iGEM life, we spent three months in wet lab.
We are proud to say that within the three months, we have achieved the following:

  1. Successfully constructed the parts for displaying recognition peptide, RPMrel, on the cell wall of Bacillus subtilis. (BBa_K733007)
  2. Successfully constructed two parts for BMP-2 synthesis and excretion using signaling peptides YbdN and YdjM respectively. (BBa_K733016, BBa_K733017)
  3. Successfully constructed a cell growth inhibitory device for regulating BMP-2 production (BBa_K733012)
  4. Successfully characterized the low efficiency promoter pTms which was used to drive the expression of antitoxin YdcD. (BBa_K733009, BBa_K733018)
  5. Successfully characterized a xylose inducible promoter which was used to control the expression of BMP-2 and toxin YdcE.
  6. Successfully characterized the cell growth inhibition device.