Team:HKUST-Hong Kong/Achievement

From 2012.igem.org

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   We are proud to say that within our three months of wet lab work we have achieved the following:</p>
   We are proud to say that within our three months of wet lab work we have achieved the following:</p>
<ol>
<ol>
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   <li>Successfully constructed the parts for displaying recognition peptide, RPMrel, on the cell wall of <i>Bacillus  subtilis</i> (<a href="http://partsregistry.org/Part:BBa_K733007" target="_blank">BBA_K733007</a>).</li>
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   <li>Successfully constructed the parts for displaying recognition peptide, RPMrel, on the cell wall of <i>Bacillus  subtilis</i> (<a href="http://partsregistry.org/Part:BBa_K733007" target="_blank">BBa_K733007</a>).</li>
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   <li>Successfully constructed two parts for BMP2 synthesis and excretion using signaling peptides YbdN and YdjM  respectively (<a href="http://partsregistry.org/Part:BBa_K733016" target="_blank">BBA_K733016</a>, <a href="http://partsregistry.org/Part:BBa_K733017" target="_blank">BBA_K733017</a>).</li>
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   <li>Successfully constructed two parts for BMP2 synthesis and excretion using signaling peptides YbdN and YdjM  respectively (<a href="http://partsregistry.org/Part:BBa_K733016" target="_blank">BBa_K733016</a>, <a href="http://partsregistry.org/Part:BBa_K733017" target="_blank">BBa_K733017</a>).</li>
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   <li>Successfully constructed a cell growth inhibition device for regulating BMP2 production (<a href="http://partsregistry.org/Part:BBa_K733012" target="_blank">BBA_K733012</a>).</li>
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   <li>Successfully constructed a cell growth inhibition device for regulating BMP2 production (<a href="http://partsregistry.org/Part:BBa_K733012" target="_blank">BBa_K733012</a>).</li>
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   <li>Successfully characterized the low efficiency promoter Ptms which was used to drive the expression of antitoxin YdcD (<a href="http://partsregistry.org/Part:BBa_K733009" target="_blank">BBA_K733009</a>, <a href="http://partsregistry.org/Part:BBa_K733018" target="_blank">BBA_K733018</a>).</li>
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   <li>Successfully characterized the low efficiency promoter Ptms which was used to drive the expression of antitoxin YdcD (<a href="http://partsregistry.org/Part:BBa_K733009" target="_blank">BBa_K733009</a>, <a href="http://partsregistry.org/Part:BBa_K733018" target="_blank">BBa_K733018</a>).</li>
   <li>Successfully characterized a xylose-inducible promoter that was used to control the expression of BMP2 and toxin YdcE.</li>
   <li>Successfully characterized a xylose-inducible promoter that was used to control the expression of BMP2 and toxin YdcE.</li>
   <li>Successfully characterized the cell growth inhibition device.</li>
   <li>Successfully characterized the cell growth inhibition device.</li>

Revision as of 16:37, 26 September 2012

Team:HKUST-Hong Kong - 2012.igem.org

Achievement

Our iGEM journey has spanned the length of six months; from the day our project was first proposed in mid-March to the day we ended wet lab work in mid-September.
We are proud to say that within our three months of wet lab work we have achieved the following:

  1. Successfully constructed the parts for displaying recognition peptide, RPMrel, on the cell wall of Bacillus subtilis (BBa_K733007).
  2. Successfully constructed two parts for BMP2 synthesis and excretion using signaling peptides YbdN and YdjM respectively (BBa_K733016, BBa_K733017).
  3. Successfully constructed a cell growth inhibition device for regulating BMP2 production (BBa_K733012).
  4. Successfully characterized the low efficiency promoter Ptms which was used to drive the expression of antitoxin YdcD (BBa_K733009, BBa_K733018).
  5. Successfully characterized a xylose-inducible promoter that was used to control the expression of BMP2 and toxin YdcE.
  6. Successfully characterized the cell growth inhibition device.