Team:HKUST-Hong Kong

From 2012.igem.org

(Difference between revisions)
 
(12 intermediate revisions not shown)
Line 17: Line 17:
     });
     });
     $('#Project').mouseleave(function(){
     $('#Project').mouseleave(function(){
-
     $('#Project').stop(true,false).animate({boxShadow:"0px 0px 0px #222222", top:"0px", left:"0px"},100);
+
     $('#Projectnction(){
-
    });
+
-
    $('#Human_practice').mouseover(function(){
+
     $('#Human_practice').stop(true,false).animate({boxShadow:"10px 10px 10px #222222", top:"-5px", left:"-5px"},100);
     $('#Human_practice').stop(true,false).animate({boxShadow:"10px 10px 10px #222222", top:"-5px", left:"-5px"},100);
     });
     });
Line 357: Line 355:
           </div>
           </div>
         </div>
         </div>
-
<div id="News_Bar" align=center style="float:right;">
 
-
<h3 id="News_Click">Click to See What's New!!!
 
-
<br> Wiki editing record below </br>
 
-
<br> http://goo.gl/H0A25 </br>
 
-
</h3>
 
-
<div id="News_Content">
 
-
  <p>
 
-
  <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project" target="_new">**Our Project Description has been uploaded, click here to view our project description!</a>  </p>
 
-
  <p>
 
-
  <a href="https://igem.org/About" target="_new">**Don't know what's iGEM? Click here to learn more!</a>
 
-
  </p>
 
-
  <p>
 
-
  <a href="https://igem.org/Team_Wikis?year=2012" target="_new">**Check out what other teams are doing!</a>
 
-
  </p>
 
-
  <p>
 
-
  <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Safety"> **Check out our page on Safety Issues!**</a>
 
-
  </p>
 
-
  <p>
 
-
  <a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Project_Abstraction"> **Click here to see our Project Abstraction!**</a>
 
-
  </p>
 
-
</div>
 
-
 
-
</div>
 
<div id="Side_Bar" align=center>
<div id="Side_Bar" align=center>
Line 440: Line 415:
<div class="Sitemap_Content">
<div class="Sitemap_Content">
<li><p><b>Wet Lab</b></p><ol>
<li><p><b>Wet Lab</b></p><ol>
-
<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Parts and Device</a></p></li>
+
<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Parts_and_Device">Parts and Devices</a></p></li>
<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook">Notebook</a></p></li>
<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Notebook">Notebook</a></p></li>
<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></li>
<li><p><a href="https://2012.igem.org/Team:HKUST-Hong_Kong/Characterization">Characterization</a></p></li>
Line 476: Line 451:
         </li>
         </li>
<li>
<li>
-
         <img src="https://static.igem.org/mediawiki/2012/c/cf/BsubtilisIntegration.JPG" width="670" height="420" />
+
         <img src="https://static.igem.org/mediawiki/2012/0/07/Whole_project.JPG" width="670" height="420" />
              
              
         </li>
         </li>
Line 485: Line 460:
         </li>
         </li>
         <li>
         <li>
-
         <img src="https://static.igem.org/mediawiki/2012/b/b8/Plasmid_Overview.jpeg" width="670" height="420" />
+
         <img src="https://static.igem.org/mediawiki/2012/c/cf/BsubtilisIntegration.JPG" width="670" height="420" />
          
          
         </li>
         </li>
Line 500: Line 475:
           <div align="left">
           <div align="left">
               <h1><p align = "center" ><font size=50>B. hercules</font><br>------<i>The Terminator of Colon Cancer</i></p></h1>
               <h1><p align = "center" ><font size=50>B. hercules</font><br>------<i>The Terminator of Colon Cancer</i></p></h1>
-
<p>Millions of cancer patients around the world currently depend on conventional cancer therapies to extend their life. These conventional therapies, composed of surgery, radiotherapy and chemotherapies, all have their own limitations and shortcomings. Short-term and long-term side effects include vomiting, hair loss, organ failure or even induction of a second tumor brought by the spreading toxicity of anti-tumor chemicals in the circulatory system, thus prompting active research into alternative cancer therapies. We, the 2012 HKUST iGEM team, have chosen to focus on colorectal carcinomas, the fourth most common cancer type around the world, as our study object. We aim to use genetically modified <i>Bacillus subtilis</i> to execute targeted drug delivery to cancer cells in the intestinal tract, offering an advantage of minimal harm of the drug to normal colon epithelial cells. <br><br> Our project hopes to enigneer <i>B. subtilis</i> to make them have the ability to recognize colon carcinomas. This specific targeting is to be achieved by expressing a colon tumor specific binding peptide on the cell wall using a cell wall binding system.<br><br> After binding, an anti-tumor chemokine is to be synthesized and secreted out from the bacterial cells with the help of a signaling peptide fused to the protein. To minimize over-production of this tumor suppressor, an inducible production system is introduced. This option of external inducible control will allow us to initiate chemokine release at a time that the effect can be most effective when the killer bacteria are closing enough to the colon cancer cells. <br><br> Finally, in consideration of both biosafety issues and the possible harm from an over-dosage of antitumor drug, a toxin-antitoxin system is to be employed in our bacterial vector. This system is supposed to provide a minimum threshold of antitumor drug production and at the same time, minimizing the risk from plasmid lateral transfer among gut flora. </p>   
+
<p>Millions of cancer patients around the world currently depend on conventional cancer therapies to extend their lives. These conventional therapies, composed of surgery, radiotherapy and chemotherapies, all have their own limitations and shortcomings. Short-term and long-term side effects include vomiting, hair loss, organ failure, or even induction of a second tumor brought about by the spreading toxicity of anti-tumor chemicals in the circulatory system, thus prompting active research into alternative cancer therapies. We, the 2012 HKUST iGEM team, have chosen to focus on colorectal carcinomas, the fourth most common cancer type around the world, as our study object. We aim to use genetically modified <i>Bacillus subtilis</i> to execute targeted drug delivery to cancer cells in the intestinal tract, offering an advantage of minimal harm of the drug to normal colon epithelial cells. <br><br> Our project hopes to engineer <i>B. subtilis</i> to enable them to recognize colon carcinomas. This specific targeting is to be achieved by expressing a colon tumor specific binding peptide on the cell wall using a cell wall binding system.<br><br> After binding, an anti-tumor chemokine is to be synthesized and secreted from the bacterial cells with the help of a signaling peptide fused to the protein. To minimize over-production of this tumor suppressor, an inducible production system is introduced. This option of external inducible control will allow us to initiate chemokine release at a time when optimum effect can be achieved; that is, when the killer bacteria are close enough to the colon cancer cells. <br><br> Finally, in consideration of both biosafety issues and the possible harm from an over-dosage of antitumor drug, a toxin-antitoxin system is to be employed in our bacterial vector. This system is supposed to provide a minimum threshold of antitumor drug production and at the same time, minimizing the risk of plasmid lateral transfer among gut flora. </p>   
           </div>
           </div>
        
        
Line 544: Line 519:
               <h1><p align = "center" ><font size=30>Why B. hercules?</font></p></h1> </div>
               <h1><p align = "center" ><font size=30>Why B. hercules?</font></p></h1> </div>
<p>
<p>
-
Cancer, as one of the most obstinate diseases around the world, is well-known for its immortal proliferation. Its name is originated from one of the Zodiac ‘Cancer’ which represents death and reincarnation. Next to Cancer, there is a constellation called Heracles. It is named after the most famous Greek hero Heracles (whose Romans name is Hercules). In Greek myth, Hera sent Karkinos (Cancer or crab in Greek) to distract Heracles in his battle with Hedra, the second labour for Heracles.  However, during the battle, Heracles easily smashed the crab’s (Cancer) shell by foot.</p>
+
Cancer, as one of the most obstinate diseases around the world, is well-known for its immortal proliferation. Its name originates from one of the Zodiac ‘Cancer’ which represents death and reincarnation. Next to Cancer, there is a constellation called Heracles. It is named after the most famous Greek hero Heracles (whose Roman name is Hercules). In Greek myth, Hera sent Karkinos (Cancer or crab in Greek) to distract Heracles in his battle with Hedra, the second labour for Heracles.  However, during the battle, Heracles easily smashed the crab’s (Cancer) shell by foot.</p>
<p>
<p>
-
B. hercules, our engineered <i>B. subtilis</i> executing anti-tumor function, is named after this hero.  We would like to have our hero, B. hercules, combat colon cancer in a breeze and eliminate his enemy, colon cancer, without harming the innocent. </p>
+
B. hercules, our engineered <i>B. subtilis</i> executing anti-tumor function, is named after this hero.  We would like to have our hero, B. hercules, combat and eliminate colon cancer in a breeze, but leaving the innocent unharmed. </p>
<p>
<p>
-
That’s the mission we given to our B. hercules.
+
That’s the mission we gave to our B. hercules.
</p>
</p>
-
          </div>         
+
                   
         </div>
         </div>
-
        <div id="paragraph3" class="bodyParagraphs">
 
-
          <div align="Right">
 
-
              <h1><p>Third Paragraph</p></h1>
 
-
          </div>
 
-
        </div>
 
<style type="text/css">
<style type="text/css">
Line 603: Line 573:
.Sitemap_Content{
.Sitemap_Content{
-
background-color:#ffffff;
+
background-color:#CCFFCC;
opacity:0.8;
opacity:0.8;
-
width:250px;
+
width:230px;
height:auto;
height:auto;
float:left;
float:left;
Line 611: Line 581:
padding-bottom:5px;
padding-bottom:5px;
padding-left:5px;
padding-left:5px;
-
border:3px solid #000000;
+
border:3px solid #99FF99;
border-radius:10px;
border-radius:10px;
-moz-border-radius:10px;
-moz-border-radius:10px;

Latest revision as of 09:53, 27 February 2013

Team:HKUST-Hong Kong - 2012.igem.org

B. hercules
------The Terminator of Colon Cancer

Millions of cancer patients around the world currently depend on conventional cancer therapies to extend their lives. These conventional therapies, composed of surgery, radiotherapy and chemotherapies, all have their own limitations and shortcomings. Short-term and long-term side effects include vomiting, hair loss, organ failure, or even induction of a second tumor brought about by the spreading toxicity of anti-tumor chemicals in the circulatory system, thus prompting active research into alternative cancer therapies. We, the 2012 HKUST iGEM team, have chosen to focus on colorectal carcinomas, the fourth most common cancer type around the world, as our study object. We aim to use genetically modified Bacillus subtilis to execute targeted drug delivery to cancer cells in the intestinal tract, offering an advantage of minimal harm of the drug to normal colon epithelial cells.

Our project hopes to engineer B. subtilis to enable them to recognize colon carcinomas. This specific targeting is to be achieved by expressing a colon tumor specific binding peptide on the cell wall using a cell wall binding system.

After binding, an anti-tumor chemokine is to be synthesized and secreted from the bacterial cells with the help of a signaling peptide fused to the protein. To minimize over-production of this tumor suppressor, an inducible production system is introduced. This option of external inducible control will allow us to initiate chemokine release at a time when optimum effect can be achieved; that is, when the killer bacteria are close enough to the colon cancer cells.

Finally, in consideration of both biosafety issues and the possible harm from an over-dosage of antitumor drug, a toxin-antitoxin system is to be employed in our bacterial vector. This system is supposed to provide a minimum threshold of antitumor drug production and at the same time, minimizing the risk of plasmid lateral transfer among gut flora.

Why B. hercules?

Cancer, as one of the most obstinate diseases around the world, is well-known for its immortal proliferation. Its name originates from one of the Zodiac ‘Cancer’ which represents death and reincarnation. Next to Cancer, there is a constellation called Heracles. It is named after the most famous Greek hero Heracles (whose Roman name is Hercules). In Greek myth, Hera sent Karkinos (Cancer or crab in Greek) to distract Heracles in his battle with Hedra, the second labour for Heracles. However, during the battle, Heracles easily smashed the crab’s (Cancer) shell by foot.

B. hercules, our engineered B. subtilis executing anti-tumor function, is named after this hero. We would like to have our hero, B. hercules, combat and eliminate colon cancer in a breeze, but leaving the innocent unharmed.

That’s the mission we gave to our B. hercules.