Team:Groningen/Notebook/Wetwork 4September2012

From 2012.igem.org







Nisa

Ligated all reporter genes (lycopene, amilCP, amilGFP) into pSac-Cm and BBa_K823023

PCR of promoters (alsT, fnr, sboA)

B. subtilis transformation for 6 gene constructs:
1. pSac-Cm+sboA+GFP
2. pSac-Cm+fnr+GFP
3. BBa_K823024+GFP
4. BBa_K823024+GFP
5. BBa_K823024
6. pSac-Cm

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