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Team:Groningen/Notebook/Wetwork 4September2012
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| - | + | Nisa <br> <br> | |
| + | Ligated all reporter genes (lycopene, amilCP, amilGFP) into pSac-Cm and BBa_K823023 <br> | ||
| + | <br> | ||
| + | PCR of promoters (alsT, fnr, sboA) <br> <br> | ||
| + | B. subtilis transformation for 6 gene constructs: <br> | ||
| + | 1. pSac-Cm+sboA+GFP <br> | ||
| + | 2. pSac-Cm+fnr+GFP <br> | ||
| + | 3. BBa_K823024+GFP <br> | ||
| + | 4. BBa_K823024+GFP <br> | ||
| + | 5. BBa_K823024 <br> | ||
| + | 6. pSac-Cm <br> | ||
<br> | <br> | ||
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
Latest revision as of 14:33, 26 September 2012
Nisa
Ligated all reporter genes (lycopene, amilCP, amilGFP) into pSac-Cm and BBa_K823023
PCR of promoters (alsT, fnr, sboA)
B. subtilis transformation for 6 gene constructs:
1. pSac-Cm+sboA+GFP
2. pSac-Cm+fnr+GFP
3. BBa_K823024+GFP
4. BBa_K823024+GFP
5. BBa_K823024
6. pSac-Cm
Back to notebook
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