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From 2012.igem.org

Arjan/Renske

Micro-Array cell harvesting

O/N culture diluted to OD = 0.326 (9:20h). Grown until OD = 0.775 (11:05).

3.6 mL brought to flask with magnet stirrer and 45 mL LB, rest put back in 37 degrees shaker for next experiment. OD = 0.095. 11:11 Micro-array harvest setup started in 37 degrees room. Stirrer on 8. Air blown over fresh meat* (kept on ice).

At 13:05 cells harvested by centrifuging (1 min, 10000 rpm, 4 degrees C) and put in -80.

12:20: remaining culture (OD = 1,78) diluted to OD = 0.311. 2 hours, 37 degrees (OD = 1,2). Brought to to flask with magnet stirrer and 45 mL LB, OD = 0.118. 13:20 Micro-array harvest setup started in 37 degrees room. Stirrer on 8. Air blown over rotten meat* (kept on ice).

• minced meat, 30% beef, 70% pork, bougth at Jumbo grocery store. Rotten meat was kept for 14 days in fridge, fresh meat was bought today.