Team:Groningen/Notebook/Wetwork 27June2012

From 2012.igem.org

(Difference between revisions)
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'''MicroArray experiment: preparations'''
'''MicroArray experiment: preparations'''
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Arjan/Renske
 
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Made setup for the micro-array experiment:
 
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37 degrees room. Flask with 50 mL culture of Bacillus subtilis sp. 168, connected with tubes to 100 mL flask with rotting/fresh meat. Filters and peristaltic pump inbetween. Culture is stirred by a magnetic stirrer.
 
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[[File:Groningen_RR_20120628_microarraysetup.jpg|thumb|400px|left|Micro-Array Setup]]
 
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Emeraldo/Nisa
Emeraldo/Nisa
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4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length.
4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length.
5.) Digestion of BBa_K090403. Result: incorrect length.
5.) Digestion of BBa_K090403. Result: incorrect length.
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 +
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Arjan/Renske
 +
 +
Made setup for the micro-array experiment:
 +
37 degrees room. Flask with 50 mL culture of Bacillus subtilis sp. 168, connected with tubes to 100 mL flask with rotting/fresh meat. Filters and peristaltic pump inbetween. Culture is stirred by a magnetic stirrer.
 +
 +
[[File:Groningen_RR_20120628_microarraysetup.jpg|thumb|400px|left|Micro-Array Setup]]

Revision as of 17:06, 8 July 2012




Tom

Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks.

Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul.

MicroArray experiment: preparations

Emeraldo/Nisa

1.) Order SpeI and EcoRI FastDigest Fermentas 2.) Order Plamid High Pure purification kit Roche 3.) B. subtilis transformation with BBa_K090403 4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length. 5.) Digestion of BBa_K090403. Result: incorrect length.


Arjan/Renske

Made setup for the micro-array experiment: 37 degrees room. Flask with 50 mL culture of Bacillus subtilis sp. 168, connected with tubes to 100 mL flask with rotting/fresh meat. Filters and peristaltic pump inbetween. Culture is stirred by a magnetic stirrer.

Micro-Array Setup