Team:Groningen/Notebook/Wetwork 27June2012
From 2012.igem.org
Line 2: | Line 2: | ||
<html> | <html> | ||
<body> | <body> | ||
- | <div style="position:absolute; right: 50px; top: | + | <div style="position:absolute; right: 50px; top: 500px;"> |
<img src="https://static.igem.org/mediawiki/2012/9/9e/Groningen2012_RR_MicArraysetup.png" width="300" height="350"> | <img src="https://static.igem.org/mediawiki/2012/9/9e/Groningen2012_RR_MicArraysetup.png" width="300" height="350"> | ||
Line 47: | Line 47: | ||
Made setup for the micro-array experiment: <br> | Made setup for the micro-array experiment: <br> | ||
- | 37 degrees room. Flask with 50 mL culture of Bacillus subtilis sp. 168,<br> | + | 37 degrees room. Flask with 50 mL culture <br> |
- | connected with tubes to 100 mL flask with rotting/fresh meat. <br> | + | of Bacillus subtilis sp. 168,<br> |
+ | connected with tubes to 100 mL flask <br> | ||
+ | with rotting/fresh meat. <br> | ||
Filters and peristaltic pump inbetween. <br> | Filters and peristaltic pump inbetween. <br> | ||
Culture is stirred by a magnetic stirrer.<br> | Culture is stirred by a magnetic stirrer.<br> |
Revision as of 16:43, 15 September 2012
Tom
Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks.
Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul.
Emeraldo/Nisa
1.) Order SpeI and EcoRI FastDigest Fermentas
2.) Order Plamid High Pure purification kit Roche
3.) B. subtilis transformation with BBa_K090403
4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length.
5.) Digestion of BBa_K090403. Result: incorrect length.
MicroArray experiment: preparations
Arjan/Renske
Made setup for the micro-array experiment:
37 degrees room. Flask with 50 mL culture
of Bacillus subtilis sp. 168,
connected with tubes to 100 mL flask
with rotting/fresh meat.
Filters and peristaltic pump inbetween.
Culture is stirred by a magnetic stirrer.
Back to notebook