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Team:Groningen/Notebook/Wetwork 27June2012
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| + | Tom <br> <br> | ||
| - | Plasmid | + | Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks. <br> <br> |
| + | Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul. <br><br> | ||
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| - | 1.) Order SpeI and EcoRI FastDigest Fermentas | + | Emeraldo/Nisa <br> |
| - | 2.) Order Plamid High Pure purification kit Roche | + | <br> |
| - | 3.) B. subtilis transformation with BBa_K090403 | + | 1.) Order SpeI and EcoRI FastDigest Fermentas<br> |
| - | 4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length. | + | 2.) Order Plamid High Pure purification kit Roche<br> |
| - | 5.) Digestion of BBa_K090403. Result: incorrect length. | + | 3.) B. subtilis transformation with BBa_K090403 <br> |
| + | 4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length. <br> | ||
| + | 5.) Digestion of BBa_K090403. Result: incorrect length.<br><br> | ||
| - | + | MicroArray experiment: preparations <br><br> | |
| - | Arjan/Renske | + | Arjan/Renske <br><br> |
| - | Made setup for the micro-array experiment: | + | Made setup for the micro-array experiment: <br> |
37 degrees room. Flask with 50 mL culture of Bacillus subtilis sp. 168, connected with tubes to 100 mL flask with rotting/fresh meat. Filters and peristaltic pump inbetween. Culture is stirred by a magnetic stirrer. | 37 degrees room. Flask with 50 mL culture of Bacillus subtilis sp. 168, connected with tubes to 100 mL flask with rotting/fresh meat. Filters and peristaltic pump inbetween. Culture is stirred by a magnetic stirrer. | ||
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| + | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
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Revision as of 16:38, 15 September 2012
</div>
Tom
Plasmid isolation of LuxR (BBa_R0062), LuxI (BBa_J37034) and backbone (BBa_090403) biobricks.
Plasmid concentrations: LuxR (1) = 99.3 ng/ul, LuxR (2) = 164.3 ng/ul, LuxI (1) = 69.9 ng/ul , LuxI (2) = 130.6 ng/ul, backbone = 63.0 ng/ul.
Emeraldo/Nisa
1.) Order SpeI and EcoRI FastDigest Fermentas
2.) Order Plamid High Pure purification kit Roche
3.) B. subtilis transformation with BBa_K090403
4.) Digestion of pigment genes from their backbones with EcoRI and SpeI. Result: the pigments genes are in correct length.
5.) Digestion of BBa_K090403. Result: incorrect length.
MicroArray experiment: preparations
Arjan/Renske
Made setup for the micro-array experiment:
37 degrees room. Flask with 50 mL culture of Bacillus subtilis sp. 168, connected with tubes to 100 mL flask with rotting/fresh meat. Filters and peristaltic pump inbetween. Culture is stirred by a magnetic stirrer.
Back to notebook
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