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Team:Groningen/Notebook/Wetwork 24July2012
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| - | Ismaêl and Wlada got an appointment with Professor Gert-Jan Euverink about the materials we might use for our sticker design. He wants to help us finding the right companies to collaborate with on a design. | + | <body><br><br><br> |
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| - | + | Tom and Arjan continued with GC. Problems with temperature settings. Made methode with 140C incubation temp. for 90 minutes. Due software problem first run was not completed. New run was set for O/n. The results were not satisfactory.<br> | |
| - | Nisa | + | <br> |
| - | + | <br> | |
| - | Digestion reaction for pSB1C3-GFP-alsTpromoter | + | Ismaêl and Wlada got an appointment with Professor Gert-Jan Euverink about the materials we might use for our sticker design. He wants to help us finding the right companies to collaborate with on a design. <br> |
| - | + | <br> | |
| - | All three incubated at 37C for 15 minutes,continue with 80C 10 minutes in the thermocycler for inactivation. | + | <br> |
| - | + | Nisa<br> | |
| - | + | <br> | |
| - | Ligation pSB1C3-GFP-alsTpromoter | + | Digestion reaction for pSB1C3-GFP-alsTpromoter<br> |
| - | + | <br> | |
| - | Make ratio backbone: insert gene= 1:4 | + | All three incubated at 37C for 15 minutes,continue with 80C 10 minutes in the thermocycler for inactivation.<br> |
| - | + | <br><br> | |
| - | Incubate at 25C for 10 minutes, continue with 80C 20 minutes in the thermocycler for inactivation. | + | Ligation pSB1C3-GFP-alsTpromoter<br> |
| - | + | <br> | |
| - | + | Make ratio backbone: insert gene= 1:4<br> | |
| - | E.coli DH5a transformation with the ligation product | + | <br> |
| - | + | Incubate at 25C for 10 minutes, continue with 80C 20 minutes in the thermocycler for inactivation. <br> | |
| - | Expectations are to have white colonies from the transformation | + | <br> |
| - | + | <br> | |
| - | + | E.coli DH5a transformation with the ligation product<br> | |
| - | < | + | <br> |
| + | Expectations are to have white colonies from the transformation<br> | ||
| + | <br> | ||
| + | <br> | ||
<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A> | ||
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{{Template:SponsorsGroningen2012}} | {{Template:SponsorsGroningen2012}} | ||
Latest revision as of 21:21, 25 September 2012
Tom and Arjan continued with GC. Problems with temperature settings. Made methode with 140C incubation temp. for 90 minutes. Due software problem first run was not completed. New run was set for O/n. The results were not satisfactory.
Ismaêl and Wlada got an appointment with Professor Gert-Jan Euverink about the materials we might use for our sticker design. He wants to help us finding the right companies to collaborate with on a design.
Nisa
Digestion reaction for pSB1C3-GFP-alsTpromoter
All three incubated at 37C for 15 minutes,continue with 80C 10 minutes in the thermocycler for inactivation.
Ligation pSB1C3-GFP-alsTpromoter
Make ratio backbone: insert gene= 1:4
Incubate at 25C for 10 minutes, continue with 80C 20 minutes in the thermocycler for inactivation.
E.coli DH5a transformation with the ligation product
Expectations are to have white colonies from the transformation
Back to notebook
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