Team:Groningen/Notebook/Wetwork 1September2012

From 2012.igem.org

(Difference between revisions)
(Created page with "{{HeaderGroningen2012}} Elbrich Miniprep of the overnight cultured transformed E.coli cells with the BioBricks from Germany. Names of the Biobricks that were used: BBa_K823021...")
Line 6: Line 6:
Miniprep of the overnight cultured transformed E.coli cells with the BioBricks from Germany. Names of the Biobricks that were used: BBa_K823021,BBa_K823023,BBa_K823024,BBa_K823026. NanoDrop concentrations were all higher than 120,5 ng/ul.
Miniprep of the overnight cultured transformed E.coli cells with the BioBricks from Germany. Names of the Biobricks that were used: BBa_K823021,BBa_K823023,BBa_K823024,BBa_K823026. NanoDrop concentrations were all higher than 120,5 ng/ul.
-
BBa_K823024 was restricted with EcoR1, Pst1, XbaI, SpeI and the sizes were checked on gel.
+
To control the cutting sites of the Bricks, BBa_K823024 was restricted with EcoR1, Pst1, XbaI, SpeI and the sizes were checked on gel.

Revision as of 14:33, 1 September 2012





Elbrich

Miniprep of the overnight cultured transformed E.coli cells with the BioBricks from Germany. Names of the Biobricks that were used: BBa_K823021,BBa_K823023,BBa_K823024,BBa_K823026. NanoDrop concentrations were all higher than 120,5 ng/ul.

To control the cutting sites of the Bricks, BBa_K823024 was restricted with EcoR1, Pst1, XbaI, SpeI and the sizes were checked on gel.