Team:Groningen/Notebook/Wetwork 18September2012

From 2012.igem.org

Revision as of 15:15, 26 September 2012 by Arjan (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)







Tom

Colony pcr with taq polymerase for the constructs: amilcp + alsT, lycopene + alsT, amilcp + fnr, lycopene + fnr, amilcp + sboA, lycopene + sboA and amilgfp + sboA.

The pcr products of amilcp + fnr, amilcp + sboA and amilgfp + sboA were succesfull. The constructs with lycopene need a longer elongation time. The primers for alsT are probably causing flaws.

Arjan

miniprep of psbic3 with alst+amilCP and SboA+cmilCP/amilGFP/lycopene. Restriction control with EcoRI and PstI. All the construct with SboA are good and will be sent tomorrow.

Back to notebook



Our sponsors:

Retrieved from "http://2012.igem.org/Team:Groningen/Notebook/Wetwork_18September2012"