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Team:Groningen/Notebook/Wetwork 18July2012
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Incubating Bacillus in LB medium for overnight growth. Next day: growth curves in SMM medium. | Incubating Bacillus in LB medium for overnight growth. Next day: growth curves in SMM medium. | ||
| + | |||
| + | |||
| + | Nisa | ||
| + | |||
| + | Possible promoter (inducible promoter) | ||
| + | 1. Strong pBAD promoter (BBa_K206000) | ||
| + | 2. LacZ regulated lambda pl hybrid promoter (BBa_R0011) | ||
| + | |||
| + | Allows for strong promotion that can be: | ||
| + | a) repressed by lacI | ||
| + | b) induced by IPTG in E.coli DH5a over a >600 fold range | ||
| + | |||
| + | |||
| + | Pactivator: pRE (BBa_K116603) or pluxR (BBa_R0062) | ||
| + | actvator: CII or luxI | ||
| + | ... and CIII cd for promoter leakiness | ||
Revision as of 14:04, 23 July 2012
Tom
Incubating Bacillus in LB medium for overnight growth. Next day: growth curves in SMM medium.
Nisa
Possible promoter (inducible promoter) 1. Strong pBAD promoter (BBa_K206000) 2. LacZ regulated lambda pl hybrid promoter (BBa_R0011)
Allows for strong promotion that can be: a) repressed by lacI b) induced by IPTG in E.coli DH5a over a >600 fold range
Pactivator: pRE (BBa_K116603) or pluxR (BBa_R0062)
actvator: CII or luxI
... and CIII cd for promoter leakiness
