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Team:Groningen/Notebook/Wetwork 15August2012
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PCR of GFP with preffix and suffix and PCR of GFP that can couple to pfu. | PCR of GFP with preffix and suffix and PCR of GFP that can couple to pfu. | ||
Result: only PCR product: GFP preffix suffix was succesfull. | Result: only PCR product: GFP preffix suffix was succesfull. | ||
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| + | Sub-culturing the transformats colonies on LB agar plates | ||
<html> | <html> | ||
Revision as of 13:58, 25 September 2012
Tom
Ligation of alsT(150, 250, 300, 500) with GFP and psb1c3 and pfu promotor with GFP and psb1c3. Result: Transformation in E.coli was succesfull.
PCR of GFP with preffix and suffix and PCR of GFP that can couple to pfu. Result: only PCR product: GFP preffix suffix was succesfull.
Emeraldo Sub-culturing the transformats colonies on LB agar plates
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