Team:Groningen/Notebook/Wetwork 11June2012


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Nisa: designed primers for construct with pigments (lycopene, violacein) from Cambrigde 2008. Further busy with design/literature study of positive feedback loop.

Literature study: search for promoter.

Transformed Violacein (BBa_K274002) into e.coli dh5a:
*2 uL of DNA into 50-75 uL of cells
*30 min on ice
*2 min, 42 degrees C
*back on ice, add 300 uL LB
*1.5 hour in 37 degrees C
*plate (LB with 10 ug/mL chloramphenicol), O/N at 37 degrees C
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