Team:Groningen/Notebook/Wetwork 10September2012

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First fluorescence microscope measurements with our pBADmeat promoters coupled to GFP.
 
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Elbrich/Renske/Tom<br>
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<br>
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First fluorescence microscope measurements with our pBADmeat promoters coupled to GFP: <br>
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grew FNR-GFP-psac-cm Bacillus subtilis in SMM in the same setup as the micro-array experiment (see 27/06 and 2/07).<br>
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results: no fluorescence measured, only autofluorescence.<br>
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Renske<br>
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ligated rfp+psac-cm (already cut by emeraldo on 9/09).<br>
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Nisa <br> <br>
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Transformation result: overgrown E. coli. Redo everything with the exact same protocol as 08/09
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<A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A>
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Latest revision as of 14:53, 26 September 2012







Elbrich/Renske/Tom

First fluorescence microscope measurements with our pBADmeat promoters coupled to GFP:
grew FNR-GFP-psac-cm Bacillus subtilis in SMM in the same setup as the micro-array experiment (see 27/06 and 2/07).
results: no fluorescence measured, only autofluorescence.


Renske

ligated rfp+psac-cm (already cut by emeraldo on 9/09).

Nisa

Transformation result: overgrown E. coli. Redo everything with the exact same protocol as 08/09

Back to notebook



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