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Team:Groningen/Notebook/Modeling 9July2012 - Revision history
2024-03-29T14:02:36Z
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Jparrish at 14:39, 26 September 2012
2012-09-26T14:39:28Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 14:39, 26 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Found a paper containing glucose and CO2 uptake rates:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> Found a paper containing glucose and CO2 uptake rates:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">* Roelco J. Kleijn, Joerg M. Buescher, Ludovic Le Chat, Matthieu Jules, Stephane Aymerich, and Uwe Sauer, "Metabolic Fluxes during Strong Carbon Catabolite Repression by Malate in Bacillus subtilis," Journal of Biological Chemistry vol. 285(3), pp. 1587–1596, 2010</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> Roelco J. Kleijn, Joerg M. Buescher, Ludovic Le Chat, Matthieu Jules, Stephane Aymerich, and Uwe Sauer, </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> "Metabolic Fluxes during Strong Carbon Catabolite Repression by Malate in Bacillus subtilis," </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> Journal of Biological Chemistry vol. 285(3), pp. 1587–1596, 2010</ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">However, still missing the relation between the glucose uptake rate and temperature. </del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> However, still missing the relation between the glucose uptake rate and temperature. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Also missing rates for glutamine, potassium, oxygen.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>Also missing rates for glutamine, potassium, oxygen.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:SponsorsGroningen2012}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Template:SponsorsGroningen2012}}</div></td></tr>
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Jparrish
http://2012.igem.org/wiki/index.php?title=Team:Groningen/Notebook/Modeling_9July2012&diff=122383&oldid=prev
Jparrish at 00:06, 18 September 2012
2012-09-18T00:06:19Z
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Jparrish
http://2012.igem.org/wiki/index.php?title=Team:Groningen/Notebook/Modeling_9July2012&diff=117817&oldid=prev
Jparrish at 16:37, 15 September 2012
2012-09-15T16:37:26Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Found a paper containing glucose and CO2 uptake rates<del class="diffchange diffchange-inline">.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <body></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"># </del>Roelco J. Kleijn, Joerg M. Buescher, Ludovic Le Chat, Matthieu Jules, Stephane Aymerich, and Uwe Sauer, "Metabolic Fluxes during Strong Carbon Catabolite Repression by Malate in Bacillus subtilis," Journal of Biological Chemistry vol. 285(3), pp. 1587–1596, 2010</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <p class="margin"></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>Found a paper containing glucose and CO2 uptake rates<ins class="diffchange diffchange-inline">:</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* </ins>Roelco J. Kleijn, Joerg M. Buescher, Ludovic Le Chat, Matthieu Jules, Stephane Aymerich, and Uwe Sauer, "Metabolic Fluxes during Strong Carbon Catabolite Repression by Malate in Bacillus subtilis," Journal of Biological Chemistry vol. 285(3), pp. 1587–1596, 2010</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> <br></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>However, still missing the relation between the glucose uptake rate and temperature. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>However, still missing the relation between the glucose uptake rate and temperature. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Also missing rates for glutamine, potassium, oxygen.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <A HREF="https://2012.igem.org/Team:Groningen/Notebook"><FONT COLOR=#ff6700>Back to notebook</FONT></A></ins></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Also missing rates for glutamine, potassium, oxygen.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">{{Template:SponsorsGroningen2012}}</ins></div></td></tr>
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Jparrish
http://2012.igem.org/wiki/index.php?title=Team:Groningen/Notebook/Modeling_9July2012&diff=29569&oldid=prev
Jparrish at 19:27, 10 July 2012
2012-07-10T19:27:28Z
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">(AP)</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">{{HeaderGroningen2012}}</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Multihost vector (BBa_I742123) double digestion with EcoRI-SpeI </del>and <del class="diffchange diffchange-inline">XbaI-PstI</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Result: Gel image of this vector is inconsistent</del>. <del class="diffchange diffchange-inline">BBa_I742123 was isolated from 3 different E</del>. <del class="diffchange diffchange-inline">coli colonies and was double-digested. The result of this reaction</del>, <del class="diffchange diffchange-inline">however</del>, <del class="diffchange diffchange-inline">varies between isolates (gel picture will be updated soon).</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Found a paper containing glucose </ins>and <ins class="diffchange diffchange-inline">CO2 uptake rates</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"># Roelco J</ins>. <ins class="diffchange diffchange-inline">Kleijn, Joerg M</ins>. <ins class="diffchange diffchange-inline">Buescher</ins>, <ins class="diffchange diffchange-inline">Ludovic Le Chat</ins>, <ins class="diffchange diffchange-inline">Matthieu Jules</ins>, <ins class="diffchange diffchange-inline">Stephane Aymerich</ins>, <ins class="diffchange diffchange-inline">and Uwe Sauer</ins>, <ins class="diffchange diffchange-inline">"Metabolic Fluxes during Strong Carbon Catabolite Repression by Malate </ins>in <ins class="diffchange diffchange-inline">Bacillus subtilis</ins>,<ins class="diffchange diffchange-inline">" Journal </ins>of <ins class="diffchange diffchange-inline">Biological Chemistry vol</ins>. <ins class="diffchange diffchange-inline">285(3), pp</ins>. <ins class="diffchange diffchange-inline">1587–1596, 2010</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">PCR of alsT promoter region using -150</del>, <del class="diffchange diffchange-inline">-250</del>, <del class="diffchange diffchange-inline">-300</del>, <del class="diffchange diffchange-inline">-500 forward primer.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">However</ins>, <ins class="diffchange diffchange-inline">still missing </ins>the <ins class="diffchange diffchange-inline">relation between </ins>the <ins class="diffchange diffchange-inline">glucose uptake rate and temperature</ins>. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Last week the annealing temperature of each primer sets has been optimized. We experienced the best result </del>in <del class="diffchange diffchange-inline">58-60oC. Today the PCR was done with different polymerase, Pfu</del>, <del class="diffchange diffchange-inline">instead </del>of <del class="diffchange diffchange-inline">Taq</del>. <del class="diffchange diffchange-inline">Annealing temperature used in this PCR was 60oC</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Also missing rates for glutamine, potassium, oxygen</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Result: Only primer pair (Fwd-150</del>,<del class="diffchange diffchange-inline">Rev) resulted in DNA fragment in </del>the <del class="diffchange diffchange-inline">0.8% agarose gel. The other primer pairs failed to give </del>the <del class="diffchange diffchange-inline">expected results</del>. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Biobrick parts that was requested from iGEM HQ finally arrived!! </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">List of biobrick parts:</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">1. BBa_K116603</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">2. BBa_K116602</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">3. BBa_J37019</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">4. BBa_K116639</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">5. BBa_K116609</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">|</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">E. coli containing these parts were inoculated into LB agar+100ug/ml Ampicilin. Incubate overnight in 37oC</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
</table>
Jparrish
http://2012.igem.org/wiki/index.php?title=Team:Groningen/Notebook/Modeling_9July2012&diff=27844&oldid=prev
Nisa.purwanto at 13:30, 9 July 2012
2012-07-09T13:30:04Z
<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 13:30, 9 July 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Last week the annealing temperature of each primer sets has been optimized. We experienced the best result in 58-60oC. Today the PCR was done with different polymerase, Pfu, instead of Taq. Annealing temperature used in this PCR was 60oC.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Last week the annealing temperature of each primer sets has been optimized. We experienced the best result in 58-60oC. Today the PCR was done with different polymerase, Pfu, instead of Taq. Annealing temperature used in this PCR was 60oC.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Result: <del class="diffchange diffchange-inline">Soon updated</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Result: <ins class="diffchange diffchange-inline">Only primer pair (Fwd-150,Rev) resulted in DNA fragment in the 0.8% agarose gel. The other primer pairs failed to give the expected results. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
</table>
Nisa.purwanto
http://2012.igem.org/wiki/index.php?title=Team:Groningen/Notebook/Modeling_9July2012&diff=27835&oldid=prev
Nisa.purwanto at 13:17, 9 July 2012
2012-07-09T13:17:21Z
<p></p>
<table style="background-color: white; color:black;">
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 13:17, 9 July 2012</td>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">(AP)</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Multihost vector (BBa_I742123) double digestion with EcoRI-SpeI and XbaI-PstI.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Multihost vector (BBa_I742123) double digestion with EcoRI-SpeI and XbaI-PstI.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|</div></td></tr>
</table>
Nisa.purwanto
http://2012.igem.org/wiki/index.php?title=Team:Groningen/Notebook/Modeling_9July2012&diff=27832&oldid=prev
Nisa.purwanto: Created page with "Multihost vector (BBa_I742123) double digestion with EcoRI-SpeI and XbaI-PstI. | Result: Gel image of this vector is inconsistent. BBa_I742123 was isolated from 3 different E. co..."
2012-07-09T13:15:42Z
<p>Created page with "Multihost vector (BBa_I742123) double digestion with EcoRI-SpeI and XbaI-PstI. | Result: Gel image of this vector is inconsistent. BBa_I742123 was isolated from 3 different E. co..."</p>
<p><b>New page</b></p><div>Multihost vector (BBa_I742123) double digestion with EcoRI-SpeI and XbaI-PstI.<br />
|<br />
Result: Gel image of this vector is inconsistent. BBa_I742123 was isolated from 3 different E. coli colonies and was double-digested. The result of this reaction, however, varies between isolates (gel picture will be updated soon).<br />
|<br />
|<br />
PCR of alsT promoter region using -150, -250, -300, -500 forward primer.<br />
|<br />
Last week the annealing temperature of each primer sets has been optimized. We experienced the best result in 58-60oC. Today the PCR was done with different polymerase, Pfu, instead of Taq. Annealing temperature used in this PCR was 60oC.<br />
|<br />
Result: Soon updated<br />
|<br />
|<br />
Biobrick parts that was requested from iGEM HQ finally arrived!! <br />
|<br />
List of biobrick parts:<br />
1. BBa_K116603<br />
2. BBa_K116602<br />
3. BBa_J37019<br />
4. BBa_K116639<br />
5. BBa_K116609<br />
|<br />
E. coli containing these parts were inoculated into LB agar+100ug/ml Ampicilin. Incubate overnight in 37oC.</div>
Nisa.purwanto