Team:Grenoble/Biology/Notebook/September
From 2012.igem.org
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<h1> Week 36: September 03<span class="exposant">rd</span> to September 09<span class="exposant">th</span> </h1> | <h1> Week 36: September 03<span class="exposant">rd</span> to September 09<span class="exposant">th</span> </h1> | ||
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<li><b>Lane 6:</b> cyaA PCR product in WT strain </li></ul></div> | <li><b>Lane 6:</b> cyaA PCR product in WT strain </li></ul></div> | ||
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<center><img src="https://static.igem.org/mediawiki/2012/2/26/20120926%282%29.png" alt="photo_gel_29"/></center> | <center><img src="https://static.igem.org/mediawiki/2012/2/26/20120926%282%29.png" alt="photo_gel_29"/></center> |
Revision as of 02:19, 27 September 2012
September
Week 36: September 03rd to September 09th
Goal of the week:
We tested the double mutant strains construction (protocol).To separate (protocol) the PCR products, we prepared a 1.8% TAE agarose gel.
Migration conditions = 100V during 30 min.
In order to reveal the DNA fragments, we used EtBr.
(the DNA ladder scale is in kb)
- Lane 1:DNA ladder (Smart Ladder)
- Lane 2: araC PCR product in WT strain
- Lane 3: araC PCR product in ΔaraC ΔcyaA strains
- Lane 4: envZ PCR product in WT strain
- Lane 5: envZ PCR product in ΔenvZ ΔcyaA strains
- Lane 6: cyaA PCR product in WT strain
(the DNA ladder scale is in kb)