Team:Exeter/lab book/3gip/wk6

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Latest revision as of 00:06, 27 September 2012

ExiGEM2012 Lab Book 3GP wk6


Protocols \| Single Gene Plasmids and Enzyme Characterisation \| Showcasing Polysaccharide Production \| The 3-Gene Inducible Plasmid Operon Construction \| Glycobase
9th - 13th July - 16th - 20th July - 23rd - 27th July - 30th July - 3rd August - 6th - 10th August - 13th - 17th August - 20th - 24th August - 27th - 31st August - 3rd - 7th September - 10th - 14th September - 17th - 21st September - Results
	The 3-Gene Inducible Plasmid: 13th - 17th August 2012 Monday 13th August See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005) Tuesday 14th August See Operon Construction 13th - 17th August of RBS-wbbC(d) (BBa_B0034 + BBa_K764005) Wednesday 15th August Morning •Miniprepping BBa_J13002 + wbnK BBa_B0014 in pSB1T3 wfcA + BBa_B0014 in pSB1T3 wbnJ + BBa_B0014 in pSB1T3 BBa_B0034 wbbC + BBa_B0014 in pSB1T3 Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed. After the neutralisation solution had been added, the eppendorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation. 40ul of water was added instead of 50ul in order to make sure the concentration of DNA increased. All were nanodropped and only wfcA + BBa_B0014 and wbnJ + BBa_B0014 gave good concentrations. Wednesday 15th August – Friday 17th August Preparation for presentation so no lab work.

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          <a href="https://2012.igem.org/Team:Exeter/lab_book/3gip/wk11"; style="color:#1d1d1b">17th - 21st September</a>
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+        <a href="https://2012.igem.org/Team:Exeter/Results/inducible"; style="color:#e30614" target="_blank"><font size="3"><b>Results</b></font></a>
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-       <font face="DokChampa" color="#57b947" size="4">
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         <p><b><u>The 3-Gene Inducible Plasmid: 13th - 17th August 2012</u></b></p>
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       <p><b><u>Monday 13th August</u></b></p>
-<p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#1d1d1b"><u>Operon Constructipn 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p>
+<p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#57B947" target="_blank"><u>Operon Construction 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p>
 <p></p><br>
 <p><b><u>Tuesday 14th August</u></b></p>
-<p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#1d1d1b"><u>Operon Constructipn 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p>
+<p>See <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk6" style="color:#57B947" target="_blank"><u>Operon Construction 13th - 17th August</u></a> of RBS-<i>wbbC</i>(d) (BBa_B0034 + BBa_K764005)</p>
 <p></p><br>
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 <p>Using the changed protocol. The tubes were centrifuged for 10 minutes to start to ensure a good pellet formed. </p>
 <p>After the neutralisation solution had been added, the eppendorfs were centrifuged for 10 minutes rather than 5 in order to get a better pellet formation. </p>
-<p>40ul of water was added instead of 40ul in order to make sure the concentration of DNA increased. </p>
+<p>40ul of water was added instead of 50ul in order to make sure the concentration of DNA increased. </p>
 <p>All were nanodropped and only <i>wfcA</i> + BBa_B0014 and <i>wbnJ</i> + BBa_B0014 gave good concentrations. </p><br>
-<p><b><u>Wednesday 15th August –Friday 17th August</u></b></p>
+<p><b><u>Wednesday 15th August – Friday 17th August</u></b></p>
-<p>Preparation of presentation so no lab work. </p>
+<p>Preparation for presentation so no lab work. </p>
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