Team:Evry/Protocols

From 2012.igem.org

(Difference between revisions)
Line 2: Line 2:
----
----
 +
<h1>PCR with Phusion polymerase<h1>
-
<u>Préparation LB et PB Agar:</u>
+
<h1>Préparation of LB medium and LB Agar:</h1>
-
 
+
<b>=> LB Agar :</b>
-
<b>=> Lb Agar :</b>
+
-18,5g LB Agar
-
-18,5g de LB Agar
+
-300ml H2O
-300ml H2O
 +
<b>=> LB medium :</b>
 +
-6g LB broth
 +
-300ml de H2O
-
 
+
Autoclaved at 250°C
-
<b>=> LB :</b>
+
-
-6g de LB broth
+
-
-300ml de H2O
+
-
-Autoclave
+

Revision as of 12:31, 3 August 2012


PCR with Phusion polymerase<h1> <h1>Préparation of LB medium and LB Agar:


=> LB Agar : -18,5g LB Agar -300ml H2O

=> LB medium : -6g LB broth -300ml de H2O

Autoclaved at 250°C















Retrieved from "http://2012.igem.org/Team:Evry/Protocols"