Team:Dundee

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     <h1 style="color: #FFFFFF;">Dundee University</h1>
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    <h2 style="color: #DDDDDD">iGem 2012</h2>
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      <li class='active '><a href='https://2012.igem.org/Team:Dundee'><span>Home</span></a></li>
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      <li class='has-sub '><a href='https://2012.igem.org/Team:Dundee/Team'><span>Team</span></a>
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            <a href="https://2012.igem.org/Team:Dundee">Home</a>
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                <li><a href='https://2012.igem.org/Team:Dundee/Team'><span>Team Members</span></a></li>
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      <li class='has-sub'><a href='https://2012.igem.org/Team:Dundee/Project'><span>Project</span></a>
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              <li><a href='https://2012.igem.org/Team:Dundee/Project'><span>The Problem</span></a></li>
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         <center><h2>Introduction</h2></center>
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        The Dundee iGem team began this project with a series of meetings during which ideas were explored, and areas of interest were discussed to find a project focus that appealed to the team. After much debating, the area of healthcare was chosen, and the project goals were refined to concentrate on a method to counteract corruption of the natural gut flora balance by clostridium difficile (<em>C.Diff</em>). Individuals undergoing long term antibiotic treatment can suffer from pseudomembranous colitis caused by <em>C.Diff</em>, and the team's aim was to modify escherichia coli (<em>E.Coli</em>) to attack <em>C.Diff</em> in a manner that restored the natural balance of the gut flora.
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<b>Six, Lyse and Obliterate: a synthetic silver bullet against healthcare acquired infection.</b><br>
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Hospital acquired infections are a global problem. One example is <I>Clostridium difficile</I>, a bacterial pathogen that infects patients undergoing prolonged antibiotic treatment and results in pseudomembranous colitis, a potentially fatal gut infection. This project aimed to design a synthetic bacterium that would respond to <I>C. difficile</I> infection and kill the pathogen <i>in situ</i>. <I>Escherichia coli</I> was engineered to secrete an endolysin from a bacteriophage that would specifically attack the <I>C. difficile</I> cell wall. The endolysin was fused to the extracellular components of an engineered Type VI Secretion System from <i>Salmonella</i>, which itself comprised 13 different proteins. In addition, a synthetic ‘inflammation biosensor’ was developed, based on a two-component system from <i>Salmonella</i>, with the aim of restricting endolysin secretion to the diseased colon only. Mathematical modelling was used to assist in the development of the laboratory work and to investigate potential therapeutic strategies beyond the scope of the experimental programme.
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Latest revision as of 21:03, 26 September 2012


Six, Lyse and Obliterate: a synthetic silver bullet against healthcare acquired infection.
Hospital acquired infections are a global problem. One example is Clostridium difficile, a bacterial pathogen that infects patients undergoing prolonged antibiotic treatment and results in pseudomembranous colitis, a potentially fatal gut infection. This project aimed to design a synthetic bacterium that would respond to C. difficile infection and kill the pathogen in situ. Escherichia coli was engineered to secrete an endolysin from a bacteriophage that would specifically attack the C. difficile cell wall. The endolysin was fused to the extracellular components of an engineered Type VI Secretion System from Salmonella, which itself comprised 13 different proteins. In addition, a synthetic ‘inflammation biosensor’ was developed, based on a two-component system from Salmonella, with the aim of restricting endolysin secretion to the diseased colon only. Mathematical modelling was used to assist in the development of the laboratory work and to investigate potential therapeutic strategies beyond the scope of the experimental programme.




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