Team:Dundee

From 2012.igem.org

(Difference between revisions)
 
(195 intermediate revisions not shown)
Line 1: Line 1:
<html>
<html>
 +
 +
<div id="igemlogo">
 +
  <a href="https://2012.igem.org/Main_Page" target="_blank">
 +
    <img src="https://static.igem.org/mediawiki/igem.org/1/10/Dundee12spacer.png" width = "975px" />
 +
  </a>
 +
</div> 
 +
<link rel="stylesheet" href="https://2012.igem.org/wiki/index.php?title=Team:Dundee/common.css&action=raw&ctype=text/css" type="text/css" />
<link rel="stylesheet" href="https://2012.igem.org/wiki/index.php?title=Team:Dundee/common.css&action=raw&ctype=text/css" type="text/css" />
-
<div id="headerText">
+
 
-
     <h1 style="color: #FFFFFF;">Dundee University</h1>
+
<div id="globalWrapper">  
-
    <br />
+
-
    <h2 style="color: #DDDDDD">iGem 2012</h2>
+
     <div id="contentMain" class="drop-shadow raised clearfix">
-
</div>
+
        <div id="menuDiv">
-
<div id="menuDiv">
+
<ul>
-
    <ul>
+
      <li class='active '><a href='https://2012.igem.org/Team:Dundee'><span>Home</span></a></li>
-
        <li>
+
      <li class='has-sub '><a href='https://2012.igem.org/Team:Dundee/Team'><span>Team</span></a>
-
            <a href="https://2012.igem.org/Team:Dundee">Home</a>
+
          <ul>
 +
                <li><a href='https://2012.igem.org/Team:Dundee/Team'><span>Team Members</span></a></li>
 +
                <li><a href='https://igem.org/Team.cgi?year=2012&team_name=Dundee'><span>iGEM team Profile</span></a></li>
 +
        </ul>
         </li>
         </li>
-
        <li>
+
      <li class='has-sub'><a href='https://2012.igem.org/Team:Dundee/Project'><span>Project</span></a>
-
            <a href="https://2012.igem.org/Team:Dundee/Team">Team</a>
+
          <ul>
-
        </li>
+
              <li><a href='https://2012.igem.org/Team:Dundee/Project'><span>The Problem</span></a></li>
-
        <li>
+
                <li><a href='https://2012.igem.org/Team:Dundee/Solution'><span>Our Solution</span></a></li>
-
            <a href="https://igem.org/Team.cgi?year=2012&team_name=Dundee">Profile</a>
+
                <li><a href="https://2012.igem.org/Team:Dundee/Biobricks"><span>Biobricks</span></a></li>
-
         </li>
+
          </ul> 
-
        <li>
+
</li>
-
             <a href="https://2012.igem.org/Team:Dundee/Project">Project</a>
+
     
-
        </li>
+
<li class='has-sub'><a href='https://2012.igem.org/Team:Dundee/Strategy'><span>Wet Lab</span></a>
-
        <li>
+
          <ul>
-
            <a href="https://2012.igem.org/Team:Dundee/Parts">Parts</a>
+
              <li><a href='https://2012.igem.org/Team:Dundee/Strategy'><span>Strategy</span></a></li>
-
        </li>
+
                <li><a href='https://2012.igem.org/Team:Dundee/Results'><span>Experimentation</span></a></li>
-
        <li>
+
</ul>
-
             <a href="https://2012.igem.org/Team:Dundee/Modeling">Modeling</a>
+
         </li>  
-
        </li>
+
      <li class='has-sub'><a href="#"><span>Dry Lab</span></a>
-
         <li>
+
             <ul>
-
             <a href="https://2012.igem.org/Team:Dundee/Notebook">Notebook</a>
+
              <li><a href='https://2012.igem.org/Team:Dundee/Modelling4'><span>Modelling</span></a></li>
-
        </li>
+
                <li><a href='https://2012.igem.org/Team:Dundee/Software'><span>Software</span></a></li>
-
        <li>
+
          </ul>
-
            <a href="https://2012.igem.org/Team:Dundee/Safety">Safety</a>
+
</li>
-
        </li>
+
      <li class='has-sub'><a href='#'><span>Human Practices</span></a>
-
        <li>
+
             <ul>
-
             <a href="https://2012.igem.org/Team:Dundee/Attributions">Attributions</a>
+
              <li><a href='https://2012.igem.org/Team:Dundee/Safety'><span>Safety</span></a></li>
-
        </li>
+
                <li><a href='https://2012.igem.org/Team:Dundee/Outreach'><span>Outreach</span></a></li>
-
    </ul>
+
          </ul>
 +
</li>
 +
         <li class='has-sub'><a href="#"><span>More</span></a>
 +
             <ul>
 +
          <li><a href='https://2012.igem.org/Team:Dundee/Collaboration'><span>Collaboration</span></a></li>
 +
                <li><a href='https://2012.igem.org/Team:Dundee/Notebook'><span>Notebook</span></a></li>
 +
                <li><a href='https://2012.igem.org/Team:Dundee/Song'><span>Song!</span></a></li>
 +
                <li><a href='https://2012.igem.org/Team:Dundee/References'><span>References</span></a></li>
 +
          </ul>
 +
</li>
 +
                 
 +
             <li class='active'><a href='https://2012.igem.org/Team:Dundee/Attributions'><span>Attributions</span></a></li>
 +
</ul>
</div>
</div>
-
<div id="mainContent">
+
       
-
    <div class="box red large centre">
+
        <!-- Start Body Content Here -->
-
         <center><h2>Introduction</h2></center>
+
       
-
         Lorem ipsum dolor sit amet, consectetur adipiscing elit. Vivamus sapien ante, suscipit vitae fringilla a, pulvinar ut augue. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Praesent mattis justo vel elit mattis vitae sodales elit sagittis. Praesent sagittis ornare justo, quis rhoncus odio sollicitudin ac. Mauris varius lorem in sapien tristique mattis. Etiam tristique adipiscing ipsum, ut imperdiet nulla elementum pharetra. Nulla lorem mi, posuere quis faucibus id, tempus ut risus. Duis ut velit sem.
+
         <h2><img src="https://static.igem.org/mediawiki/2012/f/fb/Abstract.png"></h2>
 +
<br>
 +
          
 +
<b>Six, Lyse and Obliterate: a synthetic silver bullet against healthcare acquired infection.</b><br>
 +
Hospital acquired infections are a global problem. One example is <I>Clostridium difficile</I>, a bacterial pathogen that infects patients undergoing prolonged antibiotic treatment and results in pseudomembranous colitis, a potentially fatal gut infection. This project aimed to design a synthetic bacterium that would respond to <I>C. difficile</I> infection and kill the pathogen <i>in situ</i>. <I>Escherichia coli</I> was engineered to secrete an endolysin from a bacteriophage that would specifically attack the <I>C. difficile</I> cell wall. The endolysin was fused to the extracellular components of an engineered Type VI Secretion System from <i>Salmonella</i>, which itself comprised 13 different proteins. In addition, a synthetic ‘inflammation biosensor’ was developed, based on a two-component system from <i>Salmonella</i>, with the aim of restricting endolysin secretion to the diseased colon only. Mathematical modelling was used to assist in the development of the laboratory work and to investigate potential therapeutic strategies beyond the scope of the experimental programme.
 +
<div class="clearfix"></div><br>
 +
<br>
-
Nulla venenatis, enim vitae sodales pulvinar, tortor urna aliquet dolor, vitae fringilla metus dui in enim. Cras et aliquet turpis. Pellentesque pulvinar, orci eget iaculis consectetur, arcu odio tristique ligula, eu pharetra neque lectus feugiat tellus. Integer at mi leo. Cum sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Phasellus nec dui et leo sollicitudin vulputate. Phasellus et elit lectus. Vestibulum feugiat, sapien id sodales molestie, velit magna rutrum eros, vel mattis metus dolor et purus. Suspendisse bibendum auctor eleifend. Ut accumsan odio sed nisi euismod ultricies.
+
<div style="margin-left: 40px; text-align: center;"><img style="padding: 10px;" src="https://static.igem.org/mediawiki/2012/b/b7/Homepic2.jpg"><img style="padding: 10px;" src="https://static.igem.org/mediawiki/2012/d/d0/Homepic1.jpg"><img style="padding: 10px;" src="https://static.igem.org/mediawiki/2012/e/ea/Homepic3.jpg"></div><br>
 +
<div class="clearfix">
 +
      <div class="footerbox">
 +
<br>
 +
            <center>
 +
                <img src="https://static.igem.org/mediawiki/2012/0/04/Sponsorbar2.jpg">
 +
            <center>
 +
      </div>
 +
      </div>
-
Etiam faucibus ultrices sapien pharetra hendrerit. Praesent pellentesque, leo vel dapibus cursus, tortor dui adipiscing tellus, sit amet pharetra tellus orci et libero. Fusce in orci est. Donec dapibus ultrices sapien at scelerisque. Suspendisse scelerisque arcu nibh, id mattis nulla. Phasellus sollicitudin tincidunt sapien quis laoreet. Sed quis lorem sit amet lectus commodo scelerisque ac eget nunc.
+
        <!-- End Body Content Here -->
-
     </div>
+
     </div>
</div>
</div>
-
 
+
-
<div>
+
<div id="medialinks" class="raised" style="padding: 0px; width: 34px; top: 0px;">
     <a href="http://twitter.com/#!/DundeeiGEMTeam" target="_blank">
     <a href="http://twitter.com/#!/DundeeiGEMTeam" target="_blank">
-
        <img src="https://static.igem.org/mediawiki/igem.org/e/e5/Dundee12icont.png" class="icon" alt="Follow Us On Twitter" />
+
    <img src="https://static.igem.org/mediawiki/igem.org/e/e5/Dundee12icont.png" style="padding: 2px 0 2px 0;" class="icon" width="30px" title = "Follow Us On Twitter" alt="Follow Us On Twitter" />
     </a>
     </a>
     <a href="http://www.facebook.com/pages/Dundee-University-iGEM-Team-2012/384623728265118" target="_blank">
     <a href="http://www.facebook.com/pages/Dundee-University-iGEM-Team-2012/384623728265118" target="_blank">
-
        <img src="https://static.igem.org/mediawiki/igem.org/8/83/Dundee12iconf.png" class="icon" alt="Follow Us On Facebook" />
+
    <img src="https://static.igem.org/mediawiki/igem.org/8/83/Dundee12iconf.png" style="padding: 2px 0 2px 0;" class="icon" width="30px" title="Follow Us On Facebook" alt="Follow Us On Facebook" />
     </a>
     </a>
     <a href="http://dundeeigem.blogspot.co.uk/" target="_blank">
     <a href="http://dundeeigem.blogspot.co.uk/" target="_blank">
-
        <img src="https://static.igem.org/mediawiki/igem.org/d/df/Dundee12iconb.png" class="icon" alt="Follow Our Blog" />
+
    <img src="https://static.igem.org/mediawiki/igem.org/d/df/Dundee12iconb.png" style="padding: 2px 0 2px 0;" class="icon" width="30px" title="Follow Our Blog" alt="Follow Our Blog" />
     </a>
     </a>
</div>
</div>
-
 
</html>
</html>

Latest revision as of 21:03, 26 September 2012


Six, Lyse and Obliterate: a synthetic silver bullet against healthcare acquired infection.
Hospital acquired infections are a global problem. One example is Clostridium difficile, a bacterial pathogen that infects patients undergoing prolonged antibiotic treatment and results in pseudomembranous colitis, a potentially fatal gut infection. This project aimed to design a synthetic bacterium that would respond to C. difficile infection and kill the pathogen in situ. Escherichia coli was engineered to secrete an endolysin from a bacteriophage that would specifically attack the C. difficile cell wall. The endolysin was fused to the extracellular components of an engineered Type VI Secretion System from Salmonella, which itself comprised 13 different proteins. In addition, a synthetic ‘inflammation biosensor’ was developed, based on a two-component system from Salmonella, with the aim of restricting endolysin secretion to the diseased colon only. Mathematical modelling was used to assist in the development of the laboratory work and to investigate potential therapeutic strategies beyond the scope of the experimental programme.




Retrieved from "http://2012.igem.org/Team:Dundee"