Team:Cornell/testing/project

From 2012.igem.org

(Difference between revisions)
 
(38 intermediate revisions not shown)
Line 7: Line 7:
<!--<![endif]-->
<!--<![endif]-->
<div class="row main-image">
<div class="row main-image">
-
<div class="twelve columns">
+
<div class="twelve columns no-pad" style="margin-bottom:20px;">
-
<img src="https://static.igem.org/mediawiki/2012/f/fb/Dylan_being_dylan.jpg">
+
<div id="homepage-slideshow">
 +
<img src="https://static.igem.org/mediawiki/2012/1/16/WINNER.jpg" />
 +
<img src="https://static.igem.org/mediawiki/2012/3/38/ReactorDAN.jpg" />
 +
<img src="https://static.igem.org/mediawiki/2012/3/32/IMG_0052.jpg" />
 +
<img src="https://static.igem.org/mediawiki/2012/c/c6/IMG_0045_1.jpg" />
 +
</div>
</div>
</div>
</div>
</div>
-
<div class="row">
+
<div class="row" style="margin-top:20px;">
<div class="eight columns">
<div class="eight columns">
-
<div class="row" style="margin-top:20px;">
+
<div class="row">
<div class="eleven centered columns panel">
<div class="eleven centered columns panel">
-
<h3 class="centered" style="margin:0px"> Cornell University iGEM mission statement written in twelveish words.</h3>
+
<h3 class="centered" style="margin:0px"> We have developed a novel biosensor intended for continuous monitoring of water quality in areas affected by oil and gas extraction.</h3>
</div>
</div>
</div>
</div>
<div class="row" style="margin-top:20px;">
<div class="row" style="margin-top:20px;">
<div class="twelve columns">
<div class="twelve columns">
-
Great big blurb on oil sands stuff.
+
Canadian oil sands are a vast oil reserve that, given rising prices of petroleum, are an attractive alternative to traditional sources of crude oil. However, there are numerous public health and environmental concerns regarding the oil sands extraction process. One environmental concern is the contamination of Canadian watersheds by seepage from tailings ponds. To better monitor this issue, we have engineered a novel biosensing platform with the electroactive bacterial species Shewanella oneidensis MR-1, which has the unique capability to directly transfer electrons to solid-state electrodes. We exploit this feature by genetically manipulating S. oneidensis MR-1 to upregulate its metal-reduction capacity in the presence of analyte to generate direct current output in a whole-cell biosensor. Our goal is to develop a fully autonomous electrochemical biosensor that complements the current oil sands monitoring system by providing real-time data over extended periods of time. Furthermore, our device will circumvent the costs and complications of producing and maintaining photodiode circuits used for data acquisition in bioluminescent reporter systems by instead producing a direct electrical output. While our platform is adaptable to sensing a wide range of analytes, we will initially focus on arsenic-containing compounds and naphthalene,a polycyclic aromatic hydrocarbon (PAH) – known contaminants of oil sands tailings ponds. We believe that our biosensor will be a valuable tool for remote,continuous, and long-term monitoring of pollutants in rivers and key watersheds.
-
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
-
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
-
<br />
+
-
<br />
+
-
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
+
-
 
+
</div>
</div>
</div>
</div>
-
</div>
+
<div class="row" style="margin-top:20px;">
-
<div class="row" style="margin-top:20px;">
+
<div class="ten columns centered">
-
<div class="twelve columns">
+
<ul>
-
<ul>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/overview/1">What are the Oil Sands?</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/overview/1">What are the Oil Sands?</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/overview/2">How is oil extracted from Oil Sands?</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/overview/2">How is oil extracted from Oil Sands?</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/overview/3">Environmental Concerns</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/overview/3">Environmental Concerns</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/overview/4">Health Effects from Water Contaminated with Arsenic
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/overview/4">Health Effects from Water Contaminated with Arsenic
+
and Naphthalene</a>
-
and Naphthalene</a>
+
</li>
-
</li>
+
</ul>
-
</ul>
+
</div>
</div>
</div>
</div>
</div>
-
</div>
+
<div class="four columns">
-
<div class="four columns">
+
<div class="row">
-
<div class="row">
+
<div class="twelve columns">
-
<div class="twelve columns">
+
<h4 class="centered">Wet Lab</h4>
-
<h4 class="centered">Wet Lab</h4>
+
<ul>
-
<ul>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/1">Overview</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/1">Overview</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/2">Chassis</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/2">Chassis</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
DNA Assembly
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/3">DNA Assembly</a>
+
<ul>
-
<ul>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/3/1">Arsenic Reporter</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/3/1">Arsenic reporter</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/3/2">Salicylate Reporter</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/3/2">Salicylate reporter</a>
+
</li>
-
</li>
+
</ul>
-
</ul>
+
</li>
-
</li>
+
<li>
-
<li>
+
Testing & Results
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4">Testing & results</a>
+
<ul>
-
<ul>
+
<li> <a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/7">Parts</a>
-
<li>
+
</li>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/1">Reactors</a>
+
<li>
-
</li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/1">Reactors</a>
-
<li>
+
</li>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/2">Fluorescence</a>
+
<li>
-
</li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/2">Fluorescence</a>
-
<li>
+
</li>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/3">qPCR</a>
+
<li>
-
</li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/3">qPCR</a>
-
<li>
+
</li>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/4">Western</a>
+
<li>
-
</li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/4">Western</a>
-
<li>
+
</li>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/5">Artificial River Media Growth Assays</a>
+
<li>
-
</li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/5">Artificial River Media Growth Assays</a>
-
<li>
+
</li>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/6">Naphthalene Growth Assays</a>
+
<li>
-
</li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/4/6">Naphthalene Growth Assays</a>
-
</ul>
+
</li>
-
</li>
+
</ul>
-
<li>
+
</li>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/5">Future work</a>
+
<li>
-
</li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/5">Future work</a>
-
</ul>
+
</li>
 +
<li>
 +
<a href="https://2012.igem.org/Team:Cornell/testing/project/wetlab/6">Animation</a>
 +
</li>
 +
</ul>
 +
</div>
</div>
</div>
-
</div>
+
<div class="row">
-
<div class="row">
+
<div class="twelve columns">
-
<div class="twelve columns">
+
<h4 class="centered">Dry Lab</h4>
-
<h4 class="centered">Dry Lab</h4>
+
<ul>
-
<ul>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/1">Overview</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/1">Overview</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/2">Specifications</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/2">Specifications</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/3">Design</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/3">Design</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/4">Modeling</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/4">Modeling</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/6">Animation</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/5">Assembly</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/5">Gallery</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/6">Testing and Results</a>
+
</li>
-
</li>
+
</ul>
-
<li>
+
</div>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/drylab/7">Future Work</a>
+
-
</li>
+
-
</ul>
+
</div>
</div>
-
</div>
+
<div class="row">
-
<div class="row">
+
<div class="twelve columns">
-
<div class="twelve columns">
+
<h4 class="centered">Human Practices</h4>
-
<h4 class="centered">Human Practices</h4>
+
<ul>
-
<ul>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/1">Comprehensive Environmental Assessment</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/economic">Comprehensive Environmental Analysis</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/5">Bioethics</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/fracking">Application: Oil Sands</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/2">Application: Oil Sands</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/watershed">Application: Cayuga Watershed</a>
+
</li>
-
</li>
+
<li>
-
<li>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/3">Application: Cayuga Watershed</a>
-
<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/safety">Safety Overview</a>
+
</li>
-
</li>
+
<li>
-
</ul>
+
<a href="https://2012.igem.org/Team:Cornell/testing/project/hprac/4">Safety Overview</a>
 +
</li>
 +
</ul>
 +
</div>
</div>
</div>
</div>
</div>
-
</div>
 
</div>
</div>
Line 155: Line 158:
<script type="text/javascript">
<script type="text/javascript">
$(window).load(function() {
$(window).load(function() {
 +
$('#homepage-slideshow').orbit({
 +
animation : 'horizontal-push', // fade, horizontal-slide, vertical-slide, horizontal-push
 +
animationSpeed : 800, // how fast animtions are
 +
timer : true, // true or false to have the timer
 +
resetTimerOnClick : false, // true resets the timer instead of pausing slideshow progress
 +
advanceSpeed : 8000, // if timer is enabled, time between transitions
 +
pauseOnHover : false, // if you hover pauses the slider
 +
startClockOnMouseOut : false, // if clock should start on MouseOut
 +
startClockOnMouseOutAfter : 1000, // how long after MouseOut should the timer start again
 +
directionalNav : true, // manual advancing directional navs
 +
captions : false, // do you want captions?
 +
captionAnimation : 'fade', // fade, slideOpen, none
 +
captionAnimationSpeed : 400, // if so how quickly should they animate in
 +
bullets : true, // true or false to activate the bullet navigation
 +
bulletThumbs : false, // thumbnails for the bullets
 +
bulletThumbLocation : '', // location from this file where thumbs will be
 +
afterSlideChange : function() {
 +
}, // empty function
 +
fluid : true // or set a aspect ratio for content slides (ex: '4x3')
 +
});
$('li.pg-project').addClass('active');
$('li.pg-project').addClass('active');
});
});

Latest revision as of 07:02, 17 October 2012

We have developed a novel biosensor intended for continuous monitoring of water quality in areas affected by oil and gas extraction.

Canadian oil sands are a vast oil reserve that, given rising prices of petroleum, are an attractive alternative to traditional sources of crude oil. However, there are numerous public health and environmental concerns regarding the oil sands extraction process. One environmental concern is the contamination of Canadian watersheds by seepage from tailings ponds. To better monitor this issue, we have engineered a novel biosensing platform with the electroactive bacterial species Shewanella oneidensis MR-1, which has the unique capability to directly transfer electrons to solid-state electrodes. We exploit this feature by genetically manipulating S. oneidensis MR-1 to upregulate its metal-reduction capacity in the presence of analyte to generate direct current output in a whole-cell biosensor. Our goal is to develop a fully autonomous electrochemical biosensor that complements the current oil sands monitoring system by providing real-time data over extended periods of time. Furthermore, our device will circumvent the costs and complications of producing and maintaining photodiode circuits used for data acquisition in bioluminescent reporter systems by instead producing a direct electrical output. While our platform is adaptable to sensing a wide range of analytes, we will initially focus on arsenic-containing compounds and naphthalene,a polycyclic aromatic hydrocarbon (PAH) – known contaminants of oil sands tailings ponds. We believe that our biosensor will be a valuable tool for remote,continuous, and long-term monitoring of pollutants in rivers and key watersheds.