Team:Cornell/Notebook/StrainList

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Revision as of 18:40, 26 July 2012

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Strain List and Abbreviations Used

CUGEM ID Strain Species Strain name Brief Description Plasmid conferred resistances Strain Genotype Plasmid cuID BioBrick Insert ID Backbone Name Plasmid details Storage Location Date stock made Freezer Box (# @ last census)
S0000 E. coli DH5a   N/A   N/A N/A N/A N/A      
S0001 S. oneidensis  JG700   N/A ΔmtrB N/A N/A N/A N/A Weill & Riley Robb    
S0002 S. oneidensis  JG1194   N/A ΔMtr N/A N/A N/A N/A Weill & Riley Robb    
S0003 S. oneidensis  JG1519   N/A ΔMtr/ΔmtrB N/A N/A N/A N/A Weill & Riley Robb    
S0004 S. oneidensis  JG1220   kan JG700 (S1) p1k N/A ? pmtrB Weill & Riley Robb    
S0005 S. oneidensis  JG1219   kan JG700 (S1) p2k N/A ? pmtrE Weill & Riley Robb    
S0006 S. oneidensis  JG1527   kan JG1519 (S3) p3k N/A ? pmtrB/mtrC/mtrA Weill & Riley Robb    
S0007 S. oneidensis  JG1531   kan JG1519 (S3) p4k N/A ? pmtrE/mtrC/mtrA Weill & Riley Robb    
S0008 S. oneidensis  JG1537 Backbone (control) kan JG1519 (S3) p5k N/A pBBR-BB Empty backbone Weill & Riley Robb    
S0009 E. coli DH5a Failure amp DH5a p8a N/A pBMT-1 Gibson 1 (Failed nah operon) Weill 6/27/2012 E.coli (?)
S0010 E. coli DH5a Failure amp DH5a p9a N/A pBMT-1 Gibson 2 (Failed nah operon) Weill 6/27/2012 E.coli (?)
S0011 E. coli DH5a Failure amp DH5a p10a N/A pBMT-1 Gibson 3 (Failed nah operon) Weill    
S0012 E. coli DH5a Backbone kan DH5a p11k N/A   pBBRBB-eGFP Weill    
S0013 E. coli DH5a ARS Reporter kan DH5a p12k ? pBBR-BB SP1 (arsR with BamHI in EcoRI and PstI sites on pBBRBB) Weill    
S0014 E. coli DH5a ARS Reporter kan DH5a p13k ? pBBR-BB SP2 (arsR with BamHI in EcoRI and PstI sites on pBBRBB) Weill    
S0015 E. coli DH5a ARS Reporter kan DH5a p14k ? pBBR-BB Shweta's (either SP1 or SP2?) Weill    
S0016 E. coli DH5a ARS Reporter kan DH5a p15k ? pBBR-BB TS1 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB) Weill    
S0017 E. coli DH5a ARS Reporter kan DH5a p16k ? pBBR-BB TS2 (arsR w/out BamHI in EcoRI and PstI sites on pBBRBB) Weill    
S0018 E. coli DH5a Backbone cm DH5a p17c BBa_J04450 pSB3C5 mRFP1 in MCS; p15a origin; Weill    
S0019 E. coli WM3064 Conjugation strain N/A DAP auxotroph N/A N/A     Weill 7/12/2012 E.coli (3)
S0020a S. oneidensis  CUGEM001 ARS Reporter kan JG700 (S1) p14k ?   ARS Reporter w/ RBS swap (Shweta's) Weill 7/16/2012 E.coli (2)
S0021 P. putida G7 Wildtype N/A NAH7 p20 N/A     Weill    
S0022 E. coli DH5a Salicylate sensing amp DH5a p21a BBa_K228227 pSB4A5 Salicylate sensing region (nahR and Pars); Plasmid map is not what you'd think, rely on our sequencing results. Weill    
S0023 E. coli DH5a Nitrogen sensing amp DH5a p22a BBa_K216005 pSB1A2 PyeaR promoter, responsive to nitrate, nitrite and nitric oxide Weill    
S0024 E. coli DH5a oriT amp DH5a p24a BBa_J01003 pSB1A2 Origin of transfer, homologous to mob gene in PBRBB Weill    
S0025 E. coli DH5a Promoter amp DH5a p25a BBa_J23100 BBa_J61002 Constitutive promoter (1); mRFP downsteam; pMB1 origin;   7/19/2012 E.coli (2)
S0026 E. coli DH5a Promoter amp DH5a p26a BBa_J23101 BBa_J61002 Constitutive promoter (0.7); mRFP downsteam; pMB1 origin;      
S0027 E. coli DH5a Promoter amp DH5a p27a BBa_J23106 BBa_J61002 Constitutive promoter (0.4); mRFP downsteam; pMB1 origin;      
S0028 E. coli DH5a Promoter amp DH5a p28a BBa_J23105 BBa_J61002 Constitutive promoter (0.24); mRFP downsteam; pMB1 origin;   7/19/2012 E.coli (2)
S0029 E. coli DH5a Promoter amp DH5a p29a BBa_J23114 BBa_J61002 Constitutive promoter (0.1); mRFP downsteam; pMB1 origin;      
S0030 E. coli DH5a Promoter amp DH5a p30a BBa_J23103 BBa_J61002 Constitutive promoter (0.01); mRFP downsteam; pMB1 origin;      
S0031 E. coli DH5a Backbone cm DH5a p31c BBa_J04450 pSB1C3 mRFP1 in MCS; pMB1 origin;   6/19/2012 E.coli (2)
S0032 E. coli DH5a nah Operon cm DH5a p32c   pSB1C3 nah operon in MCS; pBM1 origin;      
S0033 E. coli DH5a lac inducible mtrB kan DH5a p33k BBa_K098984 pSB3K3 lac indubible mtrB in MCS; p15a origin;      
S0034 E. coli DH5a Salicylate Reporter kan DH5a p34k   pBBR-BB        

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